The effects of serum and phorbol ester tumor promoter on ornithine decarboxylase activity in Swiss 3T3 cells

Jean M. Lockyer, Bruce E. Magun

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Addition of the tumor promotor 12-O-tetradecanoyl phobol-13-acetate (TPA) to serum-deprived mouse 3T3 cells resulted in an increase in ornithine decarboxylase activity which is maximal at 3 h and declined thereafter. Addition of serum to these same cells resulted in an ornithine decarboxylase (ODC) response which was identical to the TPA-induced response at 3 h but continued to increase to a maximum at 6 h. A variant line of 3T3 cells, which possessed phorbol ester receptors, failed to increase its ODC in response to TPA. Also, the early component of the serum-induced ODC response was markedly diminished. These data suggest that phorbol ester tumor promoters may induce an early component of a more extensive ODC response, and that a component of serum may act through the same pathway as the phorbol esters.

Original languageEnglish (US)
Pages (from-to)215-220
Number of pages6
JournalCancer Letters
Volume18
Issue number2
DOIs
StatePublished - Mar 1983

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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