The effect on immunoglobulin glycosylation of altering in vivo production of immunoglobulin G

P. Jeddi, J. Keusch, P. M. Lydyard, K. B. Bodman-Smith, Mark Chestnutt, D. Wofsy, H. Hirota, T. Taga, P. J. Delves

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effect on murine immunoglobulin G (IgG) glycosylation of altering IgG production in vivo was assessed in interleukin (IL)- 6 transgenic and CD4 knockout mice. C57BL/6 mice carrying the IL- 6 transgene showed increased levels of circulating IgG. This was associated with decreased levels of galactose on the IgG oligosaccharides. No decrease in β4-galactosyltransferase mRNA or in enzyme activity was seen in IL-6 transgenic mice. MRL- lpr/lpr mice normally have elevated levels of circulating IgG, again accompanied by decreased levels of IgG galactose. Disruption of the CD4 gene in MRL-lpr/lpr mice led to a substantial decrease in the concentration of circulating IgG, but IgG galactose levels remained low. Thus, an enforced decrease in IgG levels in the lymphoproliferative MRL-lpr/lpr mice did not alter the percentage of agalactosyl IgG in these mice, suggesting that agalactosyl IgG production is not simply caused by excessive IgG synthesis leading to an insufficient transit time in the trans-Golgi, but rather to a molecular defect in the interaction between galactosyltransferase and the immunoglobulin heavy chain.

Original languageEnglish (US)
Pages (from-to)475-480
Number of pages6
JournalImmunology
Volume98
Issue number3
DOIs
StatePublished - Nov 19 1999

Fingerprint

Glycosylation
Immunoglobulins
Immunoglobulin G
Galactose
Galactosyltransferases
Interleukin-6
Immunoglobulin Heavy Chains
Oligosaccharides
Transgenes
Inbred C57BL Mouse
Knockout Mice
Transgenic Mice
Messenger RNA

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Jeddi, P., Keusch, J., Lydyard, P. M., Bodman-Smith, K. B., Chestnutt, M., Wofsy, D., ... Delves, P. J. (1999). The effect on immunoglobulin glycosylation of altering in vivo production of immunoglobulin G. Immunology, 98(3), 475-480. https://doi.org/10.1046/j.1365-2567.1999.00896.x

The effect on immunoglobulin glycosylation of altering in vivo production of immunoglobulin G. / Jeddi, P.; Keusch, J.; Lydyard, P. M.; Bodman-Smith, K. B.; Chestnutt, Mark; Wofsy, D.; Hirota, H.; Taga, T.; Delves, P. J.

In: Immunology, Vol. 98, No. 3, 19.11.1999, p. 475-480.

Research output: Contribution to journalArticle

Jeddi, P, Keusch, J, Lydyard, PM, Bodman-Smith, KB, Chestnutt, M, Wofsy, D, Hirota, H, Taga, T & Delves, PJ 1999, 'The effect on immunoglobulin glycosylation of altering in vivo production of immunoglobulin G', Immunology, vol. 98, no. 3, pp. 475-480. https://doi.org/10.1046/j.1365-2567.1999.00896.x
Jeddi, P. ; Keusch, J. ; Lydyard, P. M. ; Bodman-Smith, K. B. ; Chestnutt, Mark ; Wofsy, D. ; Hirota, H. ; Taga, T. ; Delves, P. J. / The effect on immunoglobulin glycosylation of altering in vivo production of immunoglobulin G. In: Immunology. 1999 ; Vol. 98, No. 3. pp. 475-480.
@article{ff5afbe4730f442dbc278ed6f7b14d4b,
title = "The effect on immunoglobulin glycosylation of altering in vivo production of immunoglobulin G",
abstract = "The effect on murine immunoglobulin G (IgG) glycosylation of altering IgG production in vivo was assessed in interleukin (IL)- 6 transgenic and CD4 knockout mice. C57BL/6 mice carrying the IL- 6 transgene showed increased levels of circulating IgG. This was associated with decreased levels of galactose on the IgG oligosaccharides. No decrease in β4-galactosyltransferase mRNA or in enzyme activity was seen in IL-6 transgenic mice. MRL- lpr/lpr mice normally have elevated levels of circulating IgG, again accompanied by decreased levels of IgG galactose. Disruption of the CD4 gene in MRL-lpr/lpr mice led to a substantial decrease in the concentration of circulating IgG, but IgG galactose levels remained low. Thus, an enforced decrease in IgG levels in the lymphoproliferative MRL-lpr/lpr mice did not alter the percentage of agalactosyl IgG in these mice, suggesting that agalactosyl IgG production is not simply caused by excessive IgG synthesis leading to an insufficient transit time in the trans-Golgi, but rather to a molecular defect in the interaction between galactosyltransferase and the immunoglobulin heavy chain.",
author = "P. Jeddi and J. Keusch and Lydyard, {P. M.} and Bodman-Smith, {K. B.} and Mark Chestnutt and D. Wofsy and H. Hirota and T. Taga and Delves, {P. J.}",
year = "1999",
month = "11",
day = "19",
doi = "10.1046/j.1365-2567.1999.00896.x",
language = "English (US)",
volume = "98",
pages = "475--480",
journal = "Immunology",
issn = "0019-2805",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - The effect on immunoglobulin glycosylation of altering in vivo production of immunoglobulin G

AU - Jeddi, P.

AU - Keusch, J.

AU - Lydyard, P. M.

AU - Bodman-Smith, K. B.

AU - Chestnutt, Mark

AU - Wofsy, D.

AU - Hirota, H.

AU - Taga, T.

AU - Delves, P. J.

PY - 1999/11/19

Y1 - 1999/11/19

N2 - The effect on murine immunoglobulin G (IgG) glycosylation of altering IgG production in vivo was assessed in interleukin (IL)- 6 transgenic and CD4 knockout mice. C57BL/6 mice carrying the IL- 6 transgene showed increased levels of circulating IgG. This was associated with decreased levels of galactose on the IgG oligosaccharides. No decrease in β4-galactosyltransferase mRNA or in enzyme activity was seen in IL-6 transgenic mice. MRL- lpr/lpr mice normally have elevated levels of circulating IgG, again accompanied by decreased levels of IgG galactose. Disruption of the CD4 gene in MRL-lpr/lpr mice led to a substantial decrease in the concentration of circulating IgG, but IgG galactose levels remained low. Thus, an enforced decrease in IgG levels in the lymphoproliferative MRL-lpr/lpr mice did not alter the percentage of agalactosyl IgG in these mice, suggesting that agalactosyl IgG production is not simply caused by excessive IgG synthesis leading to an insufficient transit time in the trans-Golgi, but rather to a molecular defect in the interaction between galactosyltransferase and the immunoglobulin heavy chain.

AB - The effect on murine immunoglobulin G (IgG) glycosylation of altering IgG production in vivo was assessed in interleukin (IL)- 6 transgenic and CD4 knockout mice. C57BL/6 mice carrying the IL- 6 transgene showed increased levels of circulating IgG. This was associated with decreased levels of galactose on the IgG oligosaccharides. No decrease in β4-galactosyltransferase mRNA or in enzyme activity was seen in IL-6 transgenic mice. MRL- lpr/lpr mice normally have elevated levels of circulating IgG, again accompanied by decreased levels of IgG galactose. Disruption of the CD4 gene in MRL-lpr/lpr mice led to a substantial decrease in the concentration of circulating IgG, but IgG galactose levels remained low. Thus, an enforced decrease in IgG levels in the lymphoproliferative MRL-lpr/lpr mice did not alter the percentage of agalactosyl IgG in these mice, suggesting that agalactosyl IgG production is not simply caused by excessive IgG synthesis leading to an insufficient transit time in the trans-Golgi, but rather to a molecular defect in the interaction between galactosyltransferase and the immunoglobulin heavy chain.

UR - http://www.scopus.com/inward/record.url?scp=0032744970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032744970&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2567.1999.00896.x

DO - 10.1046/j.1365-2567.1999.00896.x

M3 - Article

C2 - 10583610

AN - SCOPUS:0032744970

VL - 98

SP - 475

EP - 480

JO - Immunology

JF - Immunology

SN - 0019-2805

IS - 3

ER -