The effect of short-term stress on serotonin gene expression in high and low resilient macaques

Cynthia Bethea, Kenny Phu, Arubala Reddy, Judy L. Cameron

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Female cynomolgus monkeys exhibit different degrees of reproductive dysfunction with moderate metabolic and psychosocial stress. When stressed with a paradigm of relocation and diet for 60. days, or 2 menstrual cycles, highly stress resilient monkeys continue to ovulate during both stress cycles (HSR); medium stress resilient monkeys ovulate once (MSR) and stress sensitive monkeys do not ovulate for the entire 60. days (SS). This study examines serotonin-related gene expression in monkeys with different sensitivity to stress and exposed to 5. days of moderate stress. Monkeys were first characterized as HSR, MSR or SS. After resumption of menstrual cycles, each monkey was re-stressed for 5. days in the early follicular phase. The expression of 3 genes pivotal to serotonin neural function was assessed in the 3 groups of monkeys (n. =4-5/group). Tryptophan hydroxylase 2 (TPH2), the serotonin reuptake transporter (SERT), and the 5HT1A autoreceptor mRNAs expression were determined at 4 morphological levels of the dorsal raphe nucleus with in situ hybridization (ISH) using digoxigenin-incorporated riboprobes. In addition, cFos was examined with immunohistochemistry. Positive pixel area and/or cell number were measured. All data were analyzed with ANOVA (3 groups) and with a t-test (2 groups). After 5. days of stress, TPH2, SERT, 5HT1A and cFos were significantly lower in the SS group than the HSR group (p.

    Original languageEnglish (US)
    Pages (from-to)143-153
    Number of pages11
    JournalProgress in Neuro-Psychopharmacology and Biological Psychiatry
    Volume44
    DOIs
    StatePublished - Jul 1 2013

    Fingerprint

    Macaca
    Haplorhini
    Serotonin
    Gene Expression
    Tryptophan Hydroxylase
    Serotonin Plasma Membrane Transport Proteins
    Menstrual Cycle
    Digoxigenin
    Autoreceptors
    Follicular Phase
    Macaca fascicularis
    In Situ Hybridization
    Analysis of Variance
    Cell Count
    Immunohistochemistry
    Diet
    Messenger RNA

    Keywords

    • Amenorrhea
    • Macaques
    • Ovulation
    • Serotonin
    • Stress
    • Stress resilient

    ASJC Scopus subject areas

    • Biological Psychiatry
    • Pharmacology

    Cite this

    The effect of short-term stress on serotonin gene expression in high and low resilient macaques. / Bethea, Cynthia; Phu, Kenny; Reddy, Arubala; Cameron, Judy L.

    In: Progress in Neuro-Psychopharmacology and Biological Psychiatry, Vol. 44, 01.07.2013, p. 143-153.

    Research output: Contribution to journalArticle

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