The effect of residual calcium in decalcified freeze-dried bone allograft in a critical-sized defect in the Rattus norvegicus calvarium.

James W. Turonis, James C. McPherson, Michael F. Cuenin, Steven Hokett, Mark E. Peacock, Mohamed Sharawy

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Demineralized freeze-dried bone allograft (DFDBA), a widely used graft material in periodontal regenerative procedures, is processed with hydrochloric acid in the attempt to expose proteins located within the bone matrixes that are capable of inducing new bone formation. However, the degree of DFDBA demineralization varies between tissue banks, which may have an effect on clinical regeneration. This study uses the critical-sized defect (CSD) model to evaluate the wound-healing response to the residual calcium of donor bone. If the percentage of residual calcium in a graft were demonstrated to significantly enhance wound healing, then periodontal patients may benefit from further standardization of human-allograft processing. Sixty adult, male, Harlan Sprague-Dawley rats (Rattus norvegicus) were randomly and equally divided into 4 test groups (ie, DFDBA at 1%, 2%, and 3% to 6% residual calcium levels and FDBA at 23% residual calcium) and a control group (no allograft). An 8-mm-diameter craniotomy was made in the rat calvarium, and polytetrafluoroethylene membranes with pore sizes of 0.50 microm were placed intracranially and ectocranially. Treatment materials were carefully placed into the CSD with a new sterilized dental amalgam carrier. Tetracycline hydrochloride was injected intraperitoneally for labeling new bone growth, and animals were euthanized 12 weeks postsurgery. As a result, histomorphometric bone fill at 12 weeks showed a statistically significant increase in the 2% DFDBA group as compared to all other groups. The authors conclude that a 2% residual calcium level in human DFDBA appears to significantly (P <or = .05) enhance osseous wound healing in the rat calvarium.

Original languageEnglish (US)
Pages (from-to)55-62
Number of pages8
JournalThe Journal of oral implantology
Volume32
Issue number2
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Skull
Allografts
Calcium
Bone and Bones
Wound Healing
Tissue Banks
Dental Amalgam
Transplants
Bone Matrix
Hydrochloric Acid
Craniotomy
Bone Development
Polytetrafluoroethylene
Tetracycline
Osteogenesis
Sprague Dawley Rats
Regeneration
Tissue Donors
Control Groups
Membranes

Cite this

The effect of residual calcium in decalcified freeze-dried bone allograft in a critical-sized defect in the Rattus norvegicus calvarium. / Turonis, James W.; McPherson, James C.; Cuenin, Michael F.; Hokett, Steven; Peacock, Mark E.; Sharawy, Mohamed.

In: The Journal of oral implantology, Vol. 32, No. 2, 2006, p. 55-62.

Research output: Contribution to journalArticle

Turonis, James W. ; McPherson, James C. ; Cuenin, Michael F. ; Hokett, Steven ; Peacock, Mark E. ; Sharawy, Mohamed. / The effect of residual calcium in decalcified freeze-dried bone allograft in a critical-sized defect in the Rattus norvegicus calvarium. In: The Journal of oral implantology. 2006 ; Vol. 32, No. 2. pp. 55-62.
@article{260f282532024315b65765cd5ac87bb7,
title = "The effect of residual calcium in decalcified freeze-dried bone allograft in a critical-sized defect in the Rattus norvegicus calvarium.",
abstract = "Demineralized freeze-dried bone allograft (DFDBA), a widely used graft material in periodontal regenerative procedures, is processed with hydrochloric acid in the attempt to expose proteins located within the bone matrixes that are capable of inducing new bone formation. However, the degree of DFDBA demineralization varies between tissue banks, which may have an effect on clinical regeneration. This study uses the critical-sized defect (CSD) model to evaluate the wound-healing response to the residual calcium of donor bone. If the percentage of residual calcium in a graft were demonstrated to significantly enhance wound healing, then periodontal patients may benefit from further standardization of human-allograft processing. Sixty adult, male, Harlan Sprague-Dawley rats (Rattus norvegicus) were randomly and equally divided into 4 test groups (ie, DFDBA at 1{\%}, 2{\%}, and 3{\%} to 6{\%} residual calcium levels and FDBA at 23{\%} residual calcium) and a control group (no allograft). An 8-mm-diameter craniotomy was made in the rat calvarium, and polytetrafluoroethylene membranes with pore sizes of 0.50 microm were placed intracranially and ectocranially. Treatment materials were carefully placed into the CSD with a new sterilized dental amalgam carrier. Tetracycline hydrochloride was injected intraperitoneally for labeling new bone growth, and animals were euthanized 12 weeks postsurgery. As a result, histomorphometric bone fill at 12 weeks showed a statistically significant increase in the 2{\%} DFDBA group as compared to all other groups. The authors conclude that a 2{\%} residual calcium level in human DFDBA appears to significantly (P",
author = "Turonis, {James W.} and McPherson, {James C.} and Cuenin, {Michael F.} and Steven Hokett and Peacock, {Mark E.} and Mohamed Sharawy",
year = "2006",
doi = "10.1563/780.1",
language = "English (US)",
volume = "32",
pages = "55--62",
journal = "Journal of Oral Implantology",
issn = "0160-6972",
publisher = "Allen Press Inc.",
number = "2",

}

TY - JOUR

T1 - The effect of residual calcium in decalcified freeze-dried bone allograft in a critical-sized defect in the Rattus norvegicus calvarium.

AU - Turonis, James W.

AU - McPherson, James C.

AU - Cuenin, Michael F.

AU - Hokett, Steven

AU - Peacock, Mark E.

AU - Sharawy, Mohamed

PY - 2006

Y1 - 2006

N2 - Demineralized freeze-dried bone allograft (DFDBA), a widely used graft material in periodontal regenerative procedures, is processed with hydrochloric acid in the attempt to expose proteins located within the bone matrixes that are capable of inducing new bone formation. However, the degree of DFDBA demineralization varies between tissue banks, which may have an effect on clinical regeneration. This study uses the critical-sized defect (CSD) model to evaluate the wound-healing response to the residual calcium of donor bone. If the percentage of residual calcium in a graft were demonstrated to significantly enhance wound healing, then periodontal patients may benefit from further standardization of human-allograft processing. Sixty adult, male, Harlan Sprague-Dawley rats (Rattus norvegicus) were randomly and equally divided into 4 test groups (ie, DFDBA at 1%, 2%, and 3% to 6% residual calcium levels and FDBA at 23% residual calcium) and a control group (no allograft). An 8-mm-diameter craniotomy was made in the rat calvarium, and polytetrafluoroethylene membranes with pore sizes of 0.50 microm were placed intracranially and ectocranially. Treatment materials were carefully placed into the CSD with a new sterilized dental amalgam carrier. Tetracycline hydrochloride was injected intraperitoneally for labeling new bone growth, and animals were euthanized 12 weeks postsurgery. As a result, histomorphometric bone fill at 12 weeks showed a statistically significant increase in the 2% DFDBA group as compared to all other groups. The authors conclude that a 2% residual calcium level in human DFDBA appears to significantly (P

AB - Demineralized freeze-dried bone allograft (DFDBA), a widely used graft material in periodontal regenerative procedures, is processed with hydrochloric acid in the attempt to expose proteins located within the bone matrixes that are capable of inducing new bone formation. However, the degree of DFDBA demineralization varies between tissue banks, which may have an effect on clinical regeneration. This study uses the critical-sized defect (CSD) model to evaluate the wound-healing response to the residual calcium of donor bone. If the percentage of residual calcium in a graft were demonstrated to significantly enhance wound healing, then periodontal patients may benefit from further standardization of human-allograft processing. Sixty adult, male, Harlan Sprague-Dawley rats (Rattus norvegicus) were randomly and equally divided into 4 test groups (ie, DFDBA at 1%, 2%, and 3% to 6% residual calcium levels and FDBA at 23% residual calcium) and a control group (no allograft). An 8-mm-diameter craniotomy was made in the rat calvarium, and polytetrafluoroethylene membranes with pore sizes of 0.50 microm were placed intracranially and ectocranially. Treatment materials were carefully placed into the CSD with a new sterilized dental amalgam carrier. Tetracycline hydrochloride was injected intraperitoneally for labeling new bone growth, and animals were euthanized 12 weeks postsurgery. As a result, histomorphometric bone fill at 12 weeks showed a statistically significant increase in the 2% DFDBA group as compared to all other groups. The authors conclude that a 2% residual calcium level in human DFDBA appears to significantly (P

UR - http://www.scopus.com/inward/record.url?scp=33744815248&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744815248&partnerID=8YFLogxK

U2 - 10.1563/780.1

DO - 10.1563/780.1

M3 - Article

VL - 32

SP - 55

EP - 62

JO - Journal of Oral Implantology

JF - Journal of Oral Implantology

SN - 0160-6972

IS - 2

ER -