The effect of fluocinolone acetonide and 12-O-tetradecanoyl-phorbol-13-acetate on the binding and biological activity of epidermal growth factor in rat fibroblasts

Jean M. Lockyer, G. Timothy Bowden, Bruce E. Magun

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Synthetic glucocorticoids such as fluocinolone acetonide (FA) have been shown to be potent inhibitors of phorbol ester-mediated tumor promotion. When Rat-1 cells were incubated with FA the cells showed an increased capacity to bind [125I]-epidermal growth factor (EGF) to surface receptors. A partial reversal of the ability of the phorbol ester tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate to reduce EGF binding to the class of high affinity receptors occurred following pre-exposure of cells to FA. The increased binding of EGF induced by FA resulted in a faster rate of degradation of EGF from the culture medium and reversed the inhibition of degradation caused by TPA. When EGF was present at low concentrations (

Original languageEnglish (US)
Pages (from-to)653-658
Number of pages6
JournalCarcinogenesis
Volume4
Issue number6
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Fluocinolone Acetonide
Fibroblasts
Growth Factors
Tetradecanoylphorbol Acetate
Bioactivity
Epidermal Growth Factor
Rats
Phorbol Esters
Receptor
Tumors
Tumor
Cell
Esters
Degradation
Two-photon Absorption
Reversal
Promoter
Carcinogens
Glucocorticoids
Inhibitor

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience
  • Cancer Research

Cite this

The effect of fluocinolone acetonide and 12-O-tetradecanoyl-phorbol-13-acetate on the binding and biological activity of epidermal growth factor in rat fibroblasts. / Lockyer, Jean M.; Bowden, G. Timothy; Magun, Bruce E.

In: Carcinogenesis, Vol. 4, No. 6, 1983, p. 653-658.

Research output: Contribution to journalArticle

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