The effect of estrogens and progestins on monkey prolactin synthesis

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    Abstract

    Dispersed monkey pituitary cells were cultured on extracellular matrix and in serum-free medium for up to 1 month, with medium changes every other day. The cells were treated with ethanol vehicle or 10 nM 17β-estradiol (E2β), 17α-estradiol (E2α), progesterone (P4), pregnenalone (P5), 17α-hydroxyprogesterone (17OH P4), and E2β plus P4. Immunoreactive PRL (IR-PRL) levels in 48 h medium samples were first determined with a homologous human PRL RIA and then with a heterologous monkey PRL RIA. On the last day of culture, the cells were incubated with [35S]methionine for 4 h, and 35S-labeled PRL in the medium and cells was determined and compared to 4 h IR-PRL. Differences between control and treated groups in 48 h IR-PRL were greater with the heterologous monkey RIA. E2β, E2α, and E2β plus P4 significantly elevated IR-PRL in 48 h medium samples an average of 73%, as determined with the monkey RIA. P4, P5, or 17OH P4 had no effect on IR-PRL in 48 h medium samples. Secreted and intracellular [35S]PRL were consistently elevated by E2β and E2β plus P4, but less consistently elevated by E2α. P4, P5, or 17OH P4 had little effect on [35S]PRL in medium or cells. IR-PRL in the 4 h medium sample and cells generally paralleled [35S]PRL, although E2α caused a more constistent elevation in IR-PRL than in [35S]PRL. In summary, E2β and E2α directly stimulate PRL synthesis by monkey pituitary cells on extracellular matrix in long term serum-free culture. Nanomolar levels of progestins have little direct effect on PRL synthesis, nor does 10 nM P4 block the stimulatory effect E2β.

    Original languageEnglish (US)
    Pages (from-to)771-779
    Number of pages9
    JournalEndocrinology
    Volume119
    Issue number2
    StatePublished - 1986

    Fingerprint

    Progestins
    Prolactin
    Haplorhini
    Estrogens
    Extracellular Matrix
    Estradiol
    17-alpha-Hydroxyprogesterone
    Serum-Free Culture Media
    Methionine
    Progesterone
    Cultured Cells
    Ethanol
    Cell Culture Techniques
    Control Groups
    Serum

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    The effect of estrogens and progestins on monkey prolactin synthesis. / Bethea, Cynthia.

    In: Endocrinology, Vol. 119, No. 2, 1986, p. 771-779.

    Research output: Contribution to journalArticle

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