The DMM complex prevents spreading of DNA methylation from transposons to nearby genes in Neurospora crassa

Shinji Honda, Zachary A. Lewis, Maite Huarte, Lucy Y. Cho, Larry David, Yang Shi, Eric U. Selker

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Transposable elements are common in genomes and must be controlled. Many organisms use DNA methylation to silence such selfish DNA, but the mechanisms that restrict the methylation to appropriate regions are largely unknown.We identified a JmjC domain protein in Neurospora,DNA METHYLATIONMODULATOR-1 (DMM-1), that prevents aberrant spreading of DNA and histone H3K9 methylation from inactivated transposons into nearby genes. Mutation of a conserved residue within the JmjC Fe(II)-binding site abolished dmm-1 function, as did mutations in conserved cysteine-rich domains. Mutants defective only in dmm-1 mutants grow poorly, but growth is restored by reduction or elimination of DNA methylation using the drug 5-azacytosine or by mutation of the DNA methyltransferase gene dim-2. DMM-1 relies on an associated protein, DMM-2, which bears a DNA-binding motif, for localization and proper function. HP1 is required to recruit the DMM complex to the edges of methylated regions.

Original languageEnglish (US)
Pages (from-to)443-454
Number of pages12
JournalGenes and Development
Volume24
Issue number5
DOIs
StatePublished - Mar 1 2010

Fingerprint

Neurospora crassa
DNA Transposable Elements
DNA Methylation
DNA
Methylation
Mutation
Genes
Neurospora
Nucleotide Motifs
Nucleic Acid Repetitive Sequences
Methyltransferases
Histones
Cysteine
Binding Sites
Genome
Growth
Pharmaceutical Preparations
Proteins

Keywords

  • DNA methylation
  • Heterochromatin
  • Histone methylation
  • HP1
  • JmjC domain

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

The DMM complex prevents spreading of DNA methylation from transposons to nearby genes in Neurospora crassa. / Honda, Shinji; Lewis, Zachary A.; Huarte, Maite; Cho, Lucy Y.; David, Larry; Shi, Yang; Selker, Eric U.

In: Genes and Development, Vol. 24, No. 5, 01.03.2010, p. 443-454.

Research output: Contribution to journalArticle

Honda, Shinji ; Lewis, Zachary A. ; Huarte, Maite ; Cho, Lucy Y. ; David, Larry ; Shi, Yang ; Selker, Eric U. / The DMM complex prevents spreading of DNA methylation from transposons to nearby genes in Neurospora crassa. In: Genes and Development. 2010 ; Vol. 24, No. 5. pp. 443-454.
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