The diagnosis of streptococcal pharyngitis by the antibody-coated bacteria test

D. L. Sewell, R. E. Bryant, A. L. Rashad, M. J. Miller

Research output: Contribution to journalArticle

Abstract

Fluorescein isothiocyanate-labeled goat antihuman immunoglobulin (anti-IgG, anti-IgA, anti-IgM) and anticomplement conjugates were used to detect antibody or complement-coated bacteria from the oropharynx of patients with pharyngitis. Throat smears prepared from patients with a positive culture for group A beta-hemolytic streptococci had significantly more bacteria that stained with labeled antihuman IgG than smears prepared from patients with a negative culture. When compared to the results of a throat culture, the sensitivity, specificity, and predictive value of a positive and negative antihuman IgG stained smear was 91%, 94%, 85%, and 96%, respectively. The results of smears stained with antihuman IgA, IgM or complement did not correlate statistically with the isolation of group A beta-hemolytic streptococci.

Original languageEnglish (US)
Pages (from-to)15-18
Number of pages4
JournalAnnals of Clinical and Laboratory Science
Volume11
Issue number1
StatePublished - Jan 1 1981

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ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology
  • Molecular Biology
  • Hematology
  • Clinical Biochemistry
  • Medical Laboratory Technology

Cite this

Sewell, D. L., Bryant, R. E., Rashad, A. L., & Miller, M. J. (1981). The diagnosis of streptococcal pharyngitis by the antibody-coated bacteria test. Annals of Clinical and Laboratory Science, 11(1), 15-18.