The diagnosis of streptococcal pharyngitis by the antibody-coated bacteria test

D. L. Sewell, Richard Bryant, A. L. Rashad, M. J. Miller

Research output: Contribution to journalArticle

Abstract

Fluorescein isothiocyanate-labeled goat antihuman immunoglobulin (anti-IgG, anti-IgA, anti-IgM) and anticomplement conjugates were used to detect antibody or complement-coated bacteria from the oropharynx of patients with pharyngitis. Throat smears prepared from patients with a positive culture for group A beta-hemolytic streptococci had significantly more bacteria that stained with labeled antihuman IgG than smears prepared from patients with a negative culture. When compared to the results of a throat culture, the sensitivity, specificity, and predictive value of a positive and negative antihuman IgG stained smear was 91%, 94%, 85%, and 96%, respectively. The results of smears stained with antihuman IgA, IgM or complement did not correlate statistically with the isolation of group A beta-hemolytic streptococci.

Original languageEnglish (US)
Pages (from-to)15-18
Number of pages4
JournalAnnals of Clinical and Laboratory Science
Volume11
Issue number1
StatePublished - 1981

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Pharyngitis
Bacteria
Immunoglobulin G
Pharynx
Streptococcus
Antibodies
Fluorescein
Immunoglobulin A
Immunoglobulin M
Immunoglobulins
Oropharynx
Goats
Sensitivity and Specificity
isothiocyanic acid
anticomplement
anti-IgM
anti-IgA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Clinical Biochemistry

Cite this

Sewell, D. L., Bryant, R., Rashad, A. L., & Miller, M. J. (1981). The diagnosis of streptococcal pharyngitis by the antibody-coated bacteria test. Annals of Clinical and Laboratory Science, 11(1), 15-18.

The diagnosis of streptococcal pharyngitis by the antibody-coated bacteria test. / Sewell, D. L.; Bryant, Richard; Rashad, A. L.; Miller, M. J.

In: Annals of Clinical and Laboratory Science, Vol. 11, No. 1, 1981, p. 15-18.

Research output: Contribution to journalArticle

Sewell, DL, Bryant, R, Rashad, AL & Miller, MJ 1981, 'The diagnosis of streptococcal pharyngitis by the antibody-coated bacteria test', Annals of Clinical and Laboratory Science, vol. 11, no. 1, pp. 15-18.
Sewell, D. L. ; Bryant, Richard ; Rashad, A. L. ; Miller, M. J. / The diagnosis of streptococcal pharyngitis by the antibody-coated bacteria test. In: Annals of Clinical and Laboratory Science. 1981 ; Vol. 11, No. 1. pp. 15-18.
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