The crystal structure of a bacterial Sufu-like protein defines a novel group of bacterial proteins that are similar to the N-terminal domain of human Sufu

Debanu Das; Robert D. Finn; Polat Abdubek; Tamara Astakhova; Herbert L. Axelrod; Constantina Bakolitsa; Xiaohui Cai; Dennis Carlton; Connie Chen; Hsiu Ju Chiu; Michelle Chiu; Thomas Clayton; Marc C. Deller; Lian Duan; Kyle Ellrott; Carol L. Farr; Julie Feuerhelm; Joanna C. Grant; Anna Grzechnik; Gye Won Han; Lukasz Jaroszewski; Kevin K. Jin; Heath E. Klock; Mark W. Knuth; Piotr Kozbial; S. Sri Krishna; Abhinav Kumar; Winnie W. Lam; David Marciano; Mitchell D. Miller; Andrew T. Morse; Edward Nigoghossian; Amanda Nopakun; Linda Okach; Christina Puckett; Ron Reyes; Henry J. Tien; Christine B. Trame; Henry Van Den Bedem; Dana Weekes; Tiffany Wooten; Qingping Xu; Andrew Yeh; Jiadong Zhou; Keith O. Hodgson; John Wooley; Marc André Elsliger; Ashley M. Deacon; Adam Godzik; Scott A. Lesley; Ian A. Wilson

doi:10.1002/pro.497

The crystal structure of a bacterial Sufu-like protein defines a novel group of bacterial proteins that are similar to the N-terminal domain of human Sufu

Debanu Das, Robert D. Finn, Polat Abdubek, Tamara Astakhova, Herbert L. Axelrod, Constantina Bakolitsa, Xiaohui Cai, Dennis Carlton, Connie Chen, Hsiu Ju Chiu, Michelle Chiu, Thomas Clayton, Marc C. Deller, Lian Duan, Kyle Ellrott, Carol L. Farr, Julie Feuerhelm, Joanna C. Grant, Anna Grzechnik, Gye Won HanLukasz Jaroszewski, Kevin K. Jin, Heath E. Klock, Mark W. Knuth, Piotr Kozbial, S. Sri Krishna, Abhinav Kumar, Winnie W. Lam, David Marciano, Mitchell D. Miller, Andrew T. Morse, Edward Nigoghossian, Amanda Nopakun, Linda Okach, Christina Puckett, Ron Reyes, Henry J. Tien, Christine B. Trame, Henry Van Den Bedem, Dana Weekes, Tiffany Wooten, Qingping Xu, Andrew Yeh, Jiadong Zhou, Keith O. Hodgson, John Wooley, Marc André Elsliger, Ashley M. Deacon, Adam Godzik, Scott A. Lesley, Ian A. Wilson

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Sufu (Suppressor of Fused), a two-domain protein, plays a critical role in regulating Hedgehog signaling and is conserved from flies to humans. A few bacterial Sufu-like proteins have previously been identified based on sequence similarity to the N-terminal domain of eukaryotic Sufu proteins, but none have been structurally or biochemically characterized and their function in bacteria is unknown. We have determined the crystal structure of a more distantly related Sufu-like homolog, NGO1391 from Neisseria gonorrhoeae, at 1.4 Å resolution, which provides the first biophysical characterization of a bacterial Sufu-like protein. The structure revealed a striking similarity to the N-terminal domain of human Sufu (r.m.s.d. of 2.6 Å over 93% of the NGO1391 protein), despite an extremely low sequence identity of ∼15%. Subsequent sequence analysis revealed that NGO1391 defines a new subset of smaller, Sufu-like proteins that are present in ∼200 bacterial species and has resulted in expansion of the SUFU (PF05076) family in Pfam. Published by Wiley-Blackwell.

Original language	English (US)
Pages (from-to)	2131-2140
Number of pages	10
Journal	Protein Science
Volume	19
Issue number	11
DOIs	https://doi.org/10.1002/pro.497
State	Published - Nov 2010
Externally published	Yes

Keywords

NGO1391
Neisseria gonorrhoeae
Pfam PF05076
Structural genomics
Sufu-like
Suppressor of fused
UniProt Q5F6Z8

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Access to Document

10.1002/pro.497

Cite this

Das, D., Finn, R. D., Abdubek, P., Astakhova, T., Axelrod, H. L., Bakolitsa, C., Cai, X., Carlton, D., Chen, C., Chiu, H. J., Chiu, M., Clayton, T., Deller, M. C., Duan, L., Ellrott, K., Farr, C. L., Feuerhelm, J., Grant, J. C., Grzechnik, A., ... Wilson, I. A. (2010). The crystal structure of a bacterial Sufu-like protein defines a novel group of bacterial proteins that are similar to the N-terminal domain of human Sufu. Protein Science, 19(11), 2131-2140. https://doi.org/10.1002/pro.497

Das, D, Finn, RD, Abdubek, P, Astakhova, T, Axelrod, HL, Bakolitsa, C, Cai, X, Carlton, D, Chen, C, Chiu, HJ, Chiu, M, Clayton, T, Deller, MC, Duan, L, Ellrott, K, Farr, CL, Feuerhelm, J, Grant, JC, Grzechnik, A, Han, GW, Jaroszewski, L, Jin, KK, Klock, HE, Knuth, MW, Kozbial, P, Sri Krishna, S, Kumar, A, Lam, WW, Marciano, D, Miller, MD, Morse, AT, Nigoghossian, E, Nopakun, A, Okach, L, Puckett, C, Reyes, R, Tien, HJ, Trame, CB, Van Den Bedem, H, Weekes, D, Wooten, T, Xu, Q, Yeh, A, Zhou, J, Hodgson, KO, Wooley, J, Elsliger, MA, Deacon, AM, Godzik, A, Lesley, SA & Wilson, IA 2010, 'The crystal structure of a bacterial Sufu-like protein defines a novel group of bacterial proteins that are similar to the N-terminal domain of human Sufu', Protein Science, vol. 19, no. 11, pp. 2131-2140. https://doi.org/10.1002/pro.497

@article{5694cfd8c87444a8ae027a4b42e1c8be,

title = "The crystal structure of a bacterial Sufu-like protein defines a novel group of bacterial proteins that are similar to the N-terminal domain of human Sufu",

abstract = "Sufu (Suppressor of Fused), a two-domain protein, plays a critical role in regulating Hedgehog signaling and is conserved from flies to humans. A few bacterial Sufu-like proteins have previously been identified based on sequence similarity to the N-terminal domain of eukaryotic Sufu proteins, but none have been structurally or biochemically characterized and their function in bacteria is unknown. We have determined the crystal structure of a more distantly related Sufu-like homolog, NGO1391 from Neisseria gonorrhoeae, at 1.4 {\AA} resolution, which provides the first biophysical characterization of a bacterial Sufu-like protein. The structure revealed a striking similarity to the N-terminal domain of human Sufu (r.m.s.d. of 2.6 {\AA} over 93% of the NGO1391 protein), despite an extremely low sequence identity of ∼15%. Subsequent sequence analysis revealed that NGO1391 defines a new subset of smaller, Sufu-like proteins that are present in ∼200 bacterial species and has resulted in expansion of the SUFU (PF05076) family in Pfam. Published by Wiley-Blackwell.",

keywords = "NGO1391, Neisseria gonorrhoeae, Pfam PF05076, Structural genomics, Sufu-like, Suppressor of fused, UniProt Q5F6Z8",

author = "Debanu Das and Finn, {Robert D.} and Polat Abdubek and Tamara Astakhova and Axelrod, {Herbert L.} and Constantina Bakolitsa and Xiaohui Cai and Dennis Carlton and Connie Chen and Chiu, {Hsiu Ju} and Michelle Chiu and Thomas Clayton and Deller, {Marc C.} and Lian Duan and Kyle Ellrott and Farr, {Carol L.} and Julie Feuerhelm and Grant, {Joanna C.} and Anna Grzechnik and Han, {Gye Won} and Lukasz Jaroszewski and Jin, {Kevin K.} and Klock, {Heath E.} and Knuth, {Mark W.} and Piotr Kozbial and {Sri Krishna}, S. and Abhinav Kumar and Lam, {Winnie W.} and David Marciano and Miller, {Mitchell D.} and Morse, {Andrew T.} and Edward Nigoghossian and Amanda Nopakun and Linda Okach and Christina Puckett and Ron Reyes and Tien, {Henry J.} and Trame, {Christine B.} and {Van Den Bedem}, Henry and Dana Weekes and Tiffany Wooten and Qingping Xu and Andrew Yeh and Jiadong Zhou and Hodgson, {Keith O.} and John Wooley and Elsliger, {Marc Andr{\'e}} and Deacon, {Ashley M.} and Adam Godzik and Lesley, {Scott A.} and Wilson, {Ian A.}",

year = "2010",

month = nov,

doi = "10.1002/pro.497",

language = "English (US)",

volume = "19",

pages = "2131--2140",

journal = "Protein Science",

issn = "0961-8368",

publisher = "Cold Spring Harbor Laboratory Press",

number = "11",

}

TY - JOUR

T1 - The crystal structure of a bacterial Sufu-like protein defines a novel group of bacterial proteins that are similar to the N-terminal domain of human Sufu

AU - Das, Debanu

AU - Finn, Robert D.

AU - Abdubek, Polat

AU - Astakhova, Tamara

AU - Axelrod, Herbert L.

AU - Bakolitsa, Constantina

AU - Cai, Xiaohui

AU - Carlton, Dennis

AU - Chen, Connie

AU - Chiu, Hsiu Ju

AU - Chiu, Michelle

AU - Clayton, Thomas

AU - Deller, Marc C.

AU - Duan, Lian

AU - Ellrott, Kyle

AU - Farr, Carol L.

AU - Feuerhelm, Julie

AU - Grant, Joanna C.

AU - Grzechnik, Anna

AU - Han, Gye Won

AU - Jaroszewski, Lukasz

AU - Jin, Kevin K.

AU - Klock, Heath E.

AU - Knuth, Mark W.

AU - Kozbial, Piotr

AU - Sri Krishna, S.

AU - Kumar, Abhinav

AU - Lam, Winnie W.

AU - Marciano, David

AU - Miller, Mitchell D.

AU - Morse, Andrew T.

AU - Nigoghossian, Edward

AU - Nopakun, Amanda

AU - Okach, Linda

AU - Puckett, Christina

AU - Reyes, Ron

AU - Tien, Henry J.

AU - Trame, Christine B.

AU - Van Den Bedem, Henry

AU - Weekes, Dana

AU - Wooten, Tiffany

AU - Xu, Qingping

AU - Yeh, Andrew

AU - Zhou, Jiadong

AU - Hodgson, Keith O.

AU - Wooley, John

AU - Elsliger, Marc André

AU - Deacon, Ashley M.

AU - Godzik, Adam

AU - Lesley, Scott A.

AU - Wilson, Ian A.

PY - 2010/11

Y1 - 2010/11

N2 - Sufu (Suppressor of Fused), a two-domain protein, plays a critical role in regulating Hedgehog signaling and is conserved from flies to humans. A few bacterial Sufu-like proteins have previously been identified based on sequence similarity to the N-terminal domain of eukaryotic Sufu proteins, but none have been structurally or biochemically characterized and their function in bacteria is unknown. We have determined the crystal structure of a more distantly related Sufu-like homolog, NGO1391 from Neisseria gonorrhoeae, at 1.4 Å resolution, which provides the first biophysical characterization of a bacterial Sufu-like protein. The structure revealed a striking similarity to the N-terminal domain of human Sufu (r.m.s.d. of 2.6 Å over 93% of the NGO1391 protein), despite an extremely low sequence identity of ∼15%. Subsequent sequence analysis revealed that NGO1391 defines a new subset of smaller, Sufu-like proteins that are present in ∼200 bacterial species and has resulted in expansion of the SUFU (PF05076) family in Pfam. Published by Wiley-Blackwell.

AB - Sufu (Suppressor of Fused), a two-domain protein, plays a critical role in regulating Hedgehog signaling and is conserved from flies to humans. A few bacterial Sufu-like proteins have previously been identified based on sequence similarity to the N-terminal domain of eukaryotic Sufu proteins, but none have been structurally or biochemically characterized and their function in bacteria is unknown. We have determined the crystal structure of a more distantly related Sufu-like homolog, NGO1391 from Neisseria gonorrhoeae, at 1.4 Å resolution, which provides the first biophysical characterization of a bacterial Sufu-like protein. The structure revealed a striking similarity to the N-terminal domain of human Sufu (r.m.s.d. of 2.6 Å over 93% of the NGO1391 protein), despite an extremely low sequence identity of ∼15%. Subsequent sequence analysis revealed that NGO1391 defines a new subset of smaller, Sufu-like proteins that are present in ∼200 bacterial species and has resulted in expansion of the SUFU (PF05076) family in Pfam. Published by Wiley-Blackwell.

KW - NGO1391

KW - Neisseria gonorrhoeae

KW - Pfam PF05076

KW - Structural genomics

KW - Sufu-like

KW - Suppressor of fused

KW - UniProt Q5F6Z8

UR - http://www.scopus.com/inward/record.url?scp=78349279938&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78349279938&partnerID=8YFLogxK

U2 - 10.1002/pro.497

DO - 10.1002/pro.497

M3 - Article

C2 - 20836087

AN - SCOPUS:78349279938

SN - 0961-8368

VL - 19

SP - 2131

EP - 2140

JO - Protein Science

JF - Protein Science

IS - 11

ER -

The crystal structure of a bacterial Sufu-like protein defines a novel group of bacterial proteins that are similar to the N-terminal domain of human Sufu

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this