The coexistence of androgen and glucocorticoid receptors in the DDT1 cloned cell line

J. S. Norris, Peter Kohler

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The hamster ductus deferens cloned tumor cell line (DDT1) has been shown to contain both androgen and glucocorticoid binding activity. The androgen receptor binding site concentration is 1.07 x 10-13 mol of testosterone/mg protein, and testosterone (T) binds with a Kd of 4.3 x 10-10M. Dihydrotestosterone (DHT) is also bound to the receptor with a Kd of 2.99 x 10-10M and the binding site concentration is 1.33 x 10-13 mol/mg protein. The order of steroid binding affinity is DHT>T>Estradiol >Progesterone. Cortisol, dexamethasone, and triamcinolone acetonide do not inhibit the androgen binding in vivo or in vitro. In a cell free system antiandrogens inhibit the binding of DHT. The DDT1 cells have a separate receptor for cortisol which binds at saturation 3.44 x 10-13 mol cortisol/mg protein and has a Kd of 4.54 x 10-9M. These studies provide evidence that these endocrine target cells contain specific high affinity receptors for more than one type of steroid. The glucocorticoid receptor may be important for maintaining essential undifferentiated functions while the DHT receptor gives the specific characteristics of sex hormone response tissues.

Original languageEnglish (US)
Pages (from-to)613-618
Number of pages6
JournalEndocrinology
Volume100
Issue number3
StatePublished - 1977
Externally publishedYes

Fingerprint

Dihydrotestosterone
Glucocorticoid Receptors
Androgen Receptors
Cell Line
Androgens
Testosterone
Steroids
Binding Sites
Androgen Antagonists
Triamcinolone Acetonide
Proteins
Endocrine Cells
Vas Deferens
Cell-Free System
Gonadal Steroid Hormones
Tumor Cell Line
Cricetinae
Dexamethasone
Glucocorticoids
Progesterone

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

The coexistence of androgen and glucocorticoid receptors in the DDT1 cloned cell line. / Norris, J. S.; Kohler, Peter.

In: Endocrinology, Vol. 100, No. 3, 1977, p. 613-618.

Research output: Contribution to journalArticle

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