The biphasic opening of the blood-brain barrier in the cortex and hippocampus after traumatic brain injury in rats

Mustafa K. Başkaya; A. Muralikrishna Rao; Aclan Doǧan; David Donaldson; Robert J. Dempsey

doi:10.1016/S0304-3940(97)00239-5

The biphasic opening of the blood-brain barrier in the cortex and hippocampus after traumatic brain injury in rats

Mustafa K. Başkaya, A. Muralikrishna Rao, Aclan Doǧan, David Donaldson, Robert J. Dempsey

Research output: Contribution to journal › Article › peer-review

258 Scopus citations

Abstract

This study examined the time course of the blood-brain barrier (BBB) opening and correlated this with brain edema formation after a lateral controlled cortical impact (CCI) brain injury in rats. Quantitative measurement of Evans-blue (EB) extravasation using fluorescence was employed at 2, 4, 6 h and 1, 2, 3, 4 and 7 days after injury. Brain edema was measured by specific gravity of the tissue at corresponding time points. Two prominent EB extravasations were observed at 4-6 h and 3-day after injury in the injury-site cortex and the ipsilateral hippocampus. Brain edema became progressively more severe over time and peaked at 24 h after injury and began to decline after day 3. These results suggest that there is a biphasic opening of the BBB after CCI brain injury and the second opening of the BBB does not contribute to a further increase in edema formation.

Original language	English (US)
Pages (from-to)	33-36
Number of pages	4
Journal	Neuroscience Letters
Volume	226
Issue number	1
DOIs	https://doi.org/10.1016/S0304-3940(97)00239-5
State	Published - Apr 18 1997
Externally published	Yes

Keywords

Blood-brain barrier
Brain edema
Controlled cortical impact
Traumatic brain injury

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0304-3940(97)00239-5

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title = "The biphasic opening of the blood-brain barrier in the cortex and hippocampus after traumatic brain injury in rats",

abstract = "This study examined the time course of the blood-brain barrier (BBB) opening and correlated this with brain edema formation after a lateral controlled cortical impact (CCI) brain injury in rats. Quantitative measurement of Evans-blue (EB) extravasation using fluorescence was employed at 2, 4, 6 h and 1, 2, 3, 4 and 7 days after injury. Brain edema was measured by specific gravity of the tissue at corresponding time points. Two prominent EB extravasations were observed at 4-6 h and 3-day after injury in the injury-site cortex and the ipsilateral hippocampus. Brain edema became progressively more severe over time and peaked at 24 h after injury and began to decline after day 3. These results suggest that there is a biphasic opening of the BBB after CCI brain injury and the second opening of the BBB does not contribute to a further increase in edema formation.",

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note = "Funding Information: This study was supported by funding from an NIH grant (PO1 NS 31220) and Department of Veterans Affairs. We carefully adhered to the animal welfare guidelines set forth in the `Guide for the Care and Use of Laboratory Animals', US Department of Health and Human Services, Pub. No. 85-23, 1985.",

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AU - Rao, A. Muralikrishna

AU - Doǧan, Aclan

AU - Donaldson, David

AU - Dempsey, Robert J.

N1 - Funding Information: This study was supported by funding from an NIH grant (PO1 NS 31220) and Department of Veterans Affairs. We carefully adhered to the animal welfare guidelines set forth in the `Guide for the Care and Use of Laboratory Animals', US Department of Health and Human Services, Pub. No. 85-23, 1985.

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N2 - This study examined the time course of the blood-brain barrier (BBB) opening and correlated this with brain edema formation after a lateral controlled cortical impact (CCI) brain injury in rats. Quantitative measurement of Evans-blue (EB) extravasation using fluorescence was employed at 2, 4, 6 h and 1, 2, 3, 4 and 7 days after injury. Brain edema was measured by specific gravity of the tissue at corresponding time points. Two prominent EB extravasations were observed at 4-6 h and 3-day after injury in the injury-site cortex and the ipsilateral hippocampus. Brain edema became progressively more severe over time and peaked at 24 h after injury and began to decline after day 3. These results suggest that there is a biphasic opening of the BBB after CCI brain injury and the second opening of the BBB does not contribute to a further increase in edema formation.

AB - This study examined the time course of the blood-brain barrier (BBB) opening and correlated this with brain edema formation after a lateral controlled cortical impact (CCI) brain injury in rats. Quantitative measurement of Evans-blue (EB) extravasation using fluorescence was employed at 2, 4, 6 h and 1, 2, 3, 4 and 7 days after injury. Brain edema was measured by specific gravity of the tissue at corresponding time points. Two prominent EB extravasations were observed at 4-6 h and 3-day after injury in the injury-site cortex and the ipsilateral hippocampus. Brain edema became progressively more severe over time and peaked at 24 h after injury and began to decline after day 3. These results suggest that there is a biphasic opening of the BBB after CCI brain injury and the second opening of the BBB does not contribute to a further increase in edema formation.

KW - Blood-brain barrier

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KW - Traumatic brain injury

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The biphasic opening of the blood-brain barrier in the cortex and hippocampus after traumatic brain injury in rats

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