The amphotropic murine leukemia virus receptor gene encodes a 71- kilodalton protein that is induced by phosphate depletion

Mei Ling Chien, John L. Foster, Janet Douglas, J. Victor Garcia

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

The amphotropic murine leukemia virus (MuLV) can infect cells from a number of mammals, including humans, via its specific receptor. Basic knowledge of amphotropic MuLV receptor expression is likely to be useful in the development and improvement of gene therapy protocols based on amphotropic-pseudotyped vectors. To investigate the expression of the human receptor for the amphotropic MuLV (GLVR-2, newly termed Pit2), we determined its mRNA levels in several cell lines and found them to vary significantly. Induction of increased levels of mRNA after removal of phosphate from the media was observed in two osteosarcoma cell lines. The increase in GLVR-2 mRNA resulted in a concomitant rise in the levels of a 71-kDa protein specifically recognized by affinity-purified antibodies against GLVR-2. Using these antibodies, we were able to confirm the intracellular topology of the large hydrophilic domain between the proposed sixth and seventh transmembrane domains of the GLVR-2 protein. This assignment is in agreement with the fourth extracellular loop being outside the cell, consistent with the proposal that the fourth extracellular loop of GLVR-2 contains the envelope binding site.

Original languageEnglish (US)
Pages (from-to)4564-4570
Number of pages7
JournalJournal of Virology
Volume71
Issue number6
StatePublished - Jun 1997
Externally publishedYes

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Type III Sodium-Phosphate Cotransporter Proteins
Murine leukemia virus
Phosphates
phosphates
Messenger RNA
cell lines
Genes
Cell Line
Murine Leukemia Viruses
receptors
Proteins
Antibody Affinity
osteosarcoma
gene therapy
genes
proteins
Osteosarcoma
Genetic Therapy
topology
binding sites

ASJC Scopus subject areas

  • Immunology

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The amphotropic murine leukemia virus receptor gene encodes a 71- kilodalton protein that is induced by phosphate depletion. / Chien, Mei Ling; Foster, John L.; Douglas, Janet; Garcia, J. Victor.

In: Journal of Virology, Vol. 71, No. 6, 06.1997, p. 4564-4570.

Research output: Contribution to journalArticle

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