Temperature-dependent blockade of nucleocytoplasmic transport of newly synthesized RNA in neurons

Robin Kleiman, Gary Banker, Oswald Steward

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

This study evaluates the temperature sensitivity of transport of recently synthesized RNA from the nucleus to the cytoplasm (nucleocytoplasmic transport) in CNS neurons. Rat hippocampal slices were incubated with [3H]uridine for 1 h to label recently synthesized RNA. Slices were then fixed immediately or maintained at 27°C or 37°C for chase intervals of 3, 4.5, and 6 h to allow for nucleocytoplasmic transport of recently synthesized RNA. The time-dependent translocation of recently synthesized RNA was evaluated autoradiographically. At the end of the 1 h pulse at either 27°C or 37°C, the label was localized exclusively over nuclei. In slices maintained at 37°C, labeling expanded to cover the cell body and proximal dendrites. However, in slices that were labeled and maintained at room temperature, labeling remained confined to the nucleus. In slices that were pulse-labeled at room temperature, and then transferred to 37°C medium, cytoplasmic labeling increased as a function of time. Nucleocytoplasmic transport of RNA in cultured rat hippocampal neurons showed a comparable temperature sensitivity. The inhibition of nucleocytoplasmic transport of RNA at room temperature provides an opportunity to evaluate neuronal function when no new RNA molecules can reach the cytoplasm.

Original languageEnglish (US)
Pages (from-to)103-109
Number of pages7
JournalMolecular Brain Research
Volume13
Issue number1-2
DOIs
StatePublished - Mar 1992

Keywords

  • Brain slice
  • Cultured neuron
  • Hippocampus
  • Nucleocytoplasmic transport
  • RNA transport
  • Temperature sensitive

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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