Temperature-dependent blockade of nucleocytoplasmic transport of newly synthesized RNA in neurons

Robin Kleiman, Gary Banker, Oswald Steward

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

This study evaluates the temperature sensitivity of transport of recently synthesized RNA from the nucleus to the cytoplasm (nucleocytoplasmic transport) in CNS neurons. Rat hippocampal slices were incubated with [3H]uridine for 1 h to label recently synthesized RNA. Slices were then fixed immediately or maintained at 27°C or 37°C for chase intervals of 3, 4.5, and 6 h to allow for nucleocytoplasmic transport of recently synthesized RNA. The time-dependent translocation of recently synthesized RNA was evaluated autoradiographically. At the end of the 1 h pulse at either 27°C or 37°C, the label was localized exclusively over nuclei. In slices maintained at 37°C, labeling expanded to cover the cell body and proximal dendrites. However, in slices that were labeled and maintained at room temperature, labeling remained confined to the nucleus. In slices that were pulse-labeled at room temperature, and then transferred to 37°C medium, cytoplasmic labeling increased as a function of time. Nucleocytoplasmic transport of RNA in cultured rat hippocampal neurons showed a comparable temperature sensitivity. The inhibition of nucleocytoplasmic transport of RNA at room temperature provides an opportunity to evaluate neuronal function when no new RNA molecules can reach the cytoplasm.

Original languageEnglish (US)
Pages (from-to)103-109
Number of pages7
JournalMolecular Brain Research
Volume13
Issue number1-2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Cell Nucleus Active Transport
RNA
Neurons
Temperature
Cytoplasm
Uridine
Dendrites

Keywords

  • Brain slice
  • Cultured neuron
  • Hippocampus
  • Nucleocytoplasmic transport
  • RNA transport
  • Temperature sensitive

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Temperature-dependent blockade of nucleocytoplasmic transport of newly synthesized RNA in neurons. / Kleiman, Robin; Banker, Gary; Steward, Oswald.

In: Molecular Brain Research, Vol. 13, No. 1-2, 1992, p. 103-109.

Research output: Contribution to journalArticle

Kleiman, Robin ; Banker, Gary ; Steward, Oswald. / Temperature-dependent blockade of nucleocytoplasmic transport of newly synthesized RNA in neurons. In: Molecular Brain Research. 1992 ; Vol. 13, No. 1-2. pp. 103-109.
@article{8d255a7e0d7b4e9d89e5a255a6a9a20f,
title = "Temperature-dependent blockade of nucleocytoplasmic transport of newly synthesized RNA in neurons",
abstract = "This study evaluates the temperature sensitivity of transport of recently synthesized RNA from the nucleus to the cytoplasm (nucleocytoplasmic transport) in CNS neurons. Rat hippocampal slices were incubated with [3H]uridine for 1 h to label recently synthesized RNA. Slices were then fixed immediately or maintained at 27°C or 37°C for chase intervals of 3, 4.5, and 6 h to allow for nucleocytoplasmic transport of recently synthesized RNA. The time-dependent translocation of recently synthesized RNA was evaluated autoradiographically. At the end of the 1 h pulse at either 27°C or 37°C, the label was localized exclusively over nuclei. In slices maintained at 37°C, labeling expanded to cover the cell body and proximal dendrites. However, in slices that were labeled and maintained at room temperature, labeling remained confined to the nucleus. In slices that were pulse-labeled at room temperature, and then transferred to 37°C medium, cytoplasmic labeling increased as a function of time. Nucleocytoplasmic transport of RNA in cultured rat hippocampal neurons showed a comparable temperature sensitivity. The inhibition of nucleocytoplasmic transport of RNA at room temperature provides an opportunity to evaluate neuronal function when no new RNA molecules can reach the cytoplasm.",
keywords = "Brain slice, Cultured neuron, Hippocampus, Nucleocytoplasmic transport, RNA transport, Temperature sensitive",
author = "Robin Kleiman and Gary Banker and Oswald Steward",
year = "1992",
doi = "10.1016/0169-328X(92)90049-H",
language = "English (US)",
volume = "13",
pages = "103--109",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Temperature-dependent blockade of nucleocytoplasmic transport of newly synthesized RNA in neurons

AU - Kleiman, Robin

AU - Banker, Gary

AU - Steward, Oswald

PY - 1992

Y1 - 1992

N2 - This study evaluates the temperature sensitivity of transport of recently synthesized RNA from the nucleus to the cytoplasm (nucleocytoplasmic transport) in CNS neurons. Rat hippocampal slices were incubated with [3H]uridine for 1 h to label recently synthesized RNA. Slices were then fixed immediately or maintained at 27°C or 37°C for chase intervals of 3, 4.5, and 6 h to allow for nucleocytoplasmic transport of recently synthesized RNA. The time-dependent translocation of recently synthesized RNA was evaluated autoradiographically. At the end of the 1 h pulse at either 27°C or 37°C, the label was localized exclusively over nuclei. In slices maintained at 37°C, labeling expanded to cover the cell body and proximal dendrites. However, in slices that were labeled and maintained at room temperature, labeling remained confined to the nucleus. In slices that were pulse-labeled at room temperature, and then transferred to 37°C medium, cytoplasmic labeling increased as a function of time. Nucleocytoplasmic transport of RNA in cultured rat hippocampal neurons showed a comparable temperature sensitivity. The inhibition of nucleocytoplasmic transport of RNA at room temperature provides an opportunity to evaluate neuronal function when no new RNA molecules can reach the cytoplasm.

AB - This study evaluates the temperature sensitivity of transport of recently synthesized RNA from the nucleus to the cytoplasm (nucleocytoplasmic transport) in CNS neurons. Rat hippocampal slices were incubated with [3H]uridine for 1 h to label recently synthesized RNA. Slices were then fixed immediately or maintained at 27°C or 37°C for chase intervals of 3, 4.5, and 6 h to allow for nucleocytoplasmic transport of recently synthesized RNA. The time-dependent translocation of recently synthesized RNA was evaluated autoradiographically. At the end of the 1 h pulse at either 27°C or 37°C, the label was localized exclusively over nuclei. In slices maintained at 37°C, labeling expanded to cover the cell body and proximal dendrites. However, in slices that were labeled and maintained at room temperature, labeling remained confined to the nucleus. In slices that were pulse-labeled at room temperature, and then transferred to 37°C medium, cytoplasmic labeling increased as a function of time. Nucleocytoplasmic transport of RNA in cultured rat hippocampal neurons showed a comparable temperature sensitivity. The inhibition of nucleocytoplasmic transport of RNA at room temperature provides an opportunity to evaluate neuronal function when no new RNA molecules can reach the cytoplasm.

KW - Brain slice

KW - Cultured neuron

KW - Hippocampus

KW - Nucleocytoplasmic transport

KW - RNA transport

KW - Temperature sensitive

UR - http://www.scopus.com/inward/record.url?scp=0026543556&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026543556&partnerID=8YFLogxK

U2 - 10.1016/0169-328X(92)90049-H

DO - 10.1016/0169-328X(92)90049-H

M3 - Article

C2 - 1374503

AN - SCOPUS:0026543556

VL - 13

SP - 103

EP - 109

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -