Targeted-microbubble binding selectively to GPIIb IIIa receptors of platelet thrombi

Patricia A. Schumann, Jonathan P. Christiansen, Rachel M. Quigley, Thomas P. McCreery, Robert H. Sweitzer, Evan C. Unger, Jonathan R. Lindner, Terry O. Matsunaga

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    181 Scopus citations

    Abstract

    RATIONALE AND OBJECTIVES. New targeted microbubbles directed to the GPIIb IIIa receptor have been developed. The objective was to determine whether targeting microbubbles to clots would enhance ultrasound imaging. Systematic studies were designed to determine whether in vitro methodology is an acceptable predictor of in vivo efficacy. MATERIALS AND METHODS. Bioconjugate ligands were inserted into lipid-coated membranes of perfluorocarbon gas microbubbles and binding studies performed on activated platelets immobilized on cell culture plates. Targeted microbubble binding to clots in a flow through chamber was also assessed. Finally, microbubble binding studies on arteriolar and venular clots in a mouse cremasteric muscle model were conducted. RESULTS. Binding studies on platelet-immobilized plates demonstrated an affinity for targeted microbubbles versus untargeted microbubbles. Semiquantitative light obscuration techniques helped to measure extent of targeted microbubble binding. Targeted microbubbles similarly bound to platelet clots in the flow model. Finally, studies in the mouse model confirmed binding of targeted microbubbles in both venules and arterioles. CONCLUSION. The use of receptor selective targeted microbubbles improved binding to vascular thrombi in both in vitro and in vivo settings.

    Original languageEnglish (US)
    Pages (from-to)587-593
    Number of pages7
    JournalInvestigative Radiology
    Volume37
    Issue number11
    DOIs
    StatePublished - Nov 1 2002

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    ASJC Scopus subject areas

    • Radiology Nuclear Medicine and imaging

    Cite this

    Schumann, P. A., Christiansen, J. P., Quigley, R. M., McCreery, T. P., Sweitzer, R. H., Unger, E. C., Lindner, J. R., & Matsunaga, T. O. (2002). Targeted-microbubble binding selectively to GPIIb IIIa receptors of platelet thrombi. Investigative Radiology, 37(11), 587-593. https://doi.org/10.1097/00004424-200211000-00001