TaCRK3 encodes a novel Theileria annulata protein kinase with motifs characteristic of the family of eukaryotic cyclin dependent kinases: A comparative analysis of its expression with TaCRK2 during the parasite life cycle

Jane Kinnaird, Mary Logan, Andrew Tait, Gordon Langsley

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The TaCRK3 gene from the bovine apicomplexan parasite Theileria annulata, encodes a 46 kDa polypeptide with strong homology to the eukaryotic family of cyclin-dependent kinases. TaCRK3 does not show significant alignment with any particular CDK group, other than the Pfmrk kinases from the related apicomplexans Plasmodium falciparum and Plasmodium yoelii. It has a putative bipartite nuclear localization signal and is located to parasite nuclei by IFAT. Protein levels are constitutive throughout differentiation of the intra-lymphocytic macroschizont. This contrasts with the expression pattern of TaCRK2 (Kinnaird et al., 1996. Mol. Microbiol., 22, 293-302) which is closely related to the eukaryotic CDK1 /2 families involved in regulation of cell cycle progression. TaCRK2 is also located to the parasite nuclei but has no nuclear localization signal and exhibits transient up-regulation in protein levels during mid-merogony. However compared to TaCRK3, it shows down-regulation near the end of merogony. We predict that TaCRK3 may have a role in regulation of gene transcription while TaCRK2 is more likely to be involved in control of parasite nuclear division.

Original languageEnglish (US)
Pages (from-to)127-135
Number of pages9
JournalGene
Volume279
Issue number2
DOIs
StatePublished - Nov 28 2001
Externally publishedYes

Fingerprint

Theileria annulata
Cyclin-Dependent Kinases
Life Cycle Stages
Protein Kinases
Nuclear Localization Signals
Parasites
Plasmodium yoelii
Cell Nucleus Division
Communicable Disease Control
Plasmodium falciparum
Genes
Cell Cycle
Proteins
Phosphotransferases
Up-Regulation
Down-Regulation
Peptides

Keywords

  • Apicomplexa
  • Cyclin-dependent kinases
  • Differentiation
  • Nuclear localization
  • Theileria

ASJC Scopus subject areas

  • Genetics

Cite this

@article{d5986ff8486046b99d81c78f1f26aec9,
title = "TaCRK3 encodes a novel Theileria annulata protein kinase with motifs characteristic of the family of eukaryotic cyclin dependent kinases: A comparative analysis of its expression with TaCRK2 during the parasite life cycle",
abstract = "The TaCRK3 gene from the bovine apicomplexan parasite Theileria annulata, encodes a 46 kDa polypeptide with strong homology to the eukaryotic family of cyclin-dependent kinases. TaCRK3 does not show significant alignment with any particular CDK group, other than the Pfmrk kinases from the related apicomplexans Plasmodium falciparum and Plasmodium yoelii. It has a putative bipartite nuclear localization signal and is located to parasite nuclei by IFAT. Protein levels are constitutive throughout differentiation of the intra-lymphocytic macroschizont. This contrasts with the expression pattern of TaCRK2 (Kinnaird et al., 1996. Mol. Microbiol., 22, 293-302) which is closely related to the eukaryotic CDK1 /2 families involved in regulation of cell cycle progression. TaCRK2 is also located to the parasite nuclei but has no nuclear localization signal and exhibits transient up-regulation in protein levels during mid-merogony. However compared to TaCRK3, it shows down-regulation near the end of merogony. We predict that TaCRK3 may have a role in regulation of gene transcription while TaCRK2 is more likely to be involved in control of parasite nuclear division.",
keywords = "Apicomplexa, Cyclin-dependent kinases, Differentiation, Nuclear localization, Theileria",
author = "Jane Kinnaird and Mary Logan and Andrew Tait and Gordon Langsley",
year = "2001",
month = "11",
day = "28",
doi = "10.1016/S0378-1119(01)00753-3",
language = "English (US)",
volume = "279",
pages = "127--135",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - TaCRK3 encodes a novel Theileria annulata protein kinase with motifs characteristic of the family of eukaryotic cyclin dependent kinases

T2 - A comparative analysis of its expression with TaCRK2 during the parasite life cycle

AU - Kinnaird, Jane

AU - Logan, Mary

AU - Tait, Andrew

AU - Langsley, Gordon

PY - 2001/11/28

Y1 - 2001/11/28

N2 - The TaCRK3 gene from the bovine apicomplexan parasite Theileria annulata, encodes a 46 kDa polypeptide with strong homology to the eukaryotic family of cyclin-dependent kinases. TaCRK3 does not show significant alignment with any particular CDK group, other than the Pfmrk kinases from the related apicomplexans Plasmodium falciparum and Plasmodium yoelii. It has a putative bipartite nuclear localization signal and is located to parasite nuclei by IFAT. Protein levels are constitutive throughout differentiation of the intra-lymphocytic macroschizont. This contrasts with the expression pattern of TaCRK2 (Kinnaird et al., 1996. Mol. Microbiol., 22, 293-302) which is closely related to the eukaryotic CDK1 /2 families involved in regulation of cell cycle progression. TaCRK2 is also located to the parasite nuclei but has no nuclear localization signal and exhibits transient up-regulation in protein levels during mid-merogony. However compared to TaCRK3, it shows down-regulation near the end of merogony. We predict that TaCRK3 may have a role in regulation of gene transcription while TaCRK2 is more likely to be involved in control of parasite nuclear division.

AB - The TaCRK3 gene from the bovine apicomplexan parasite Theileria annulata, encodes a 46 kDa polypeptide with strong homology to the eukaryotic family of cyclin-dependent kinases. TaCRK3 does not show significant alignment with any particular CDK group, other than the Pfmrk kinases from the related apicomplexans Plasmodium falciparum and Plasmodium yoelii. It has a putative bipartite nuclear localization signal and is located to parasite nuclei by IFAT. Protein levels are constitutive throughout differentiation of the intra-lymphocytic macroschizont. This contrasts with the expression pattern of TaCRK2 (Kinnaird et al., 1996. Mol. Microbiol., 22, 293-302) which is closely related to the eukaryotic CDK1 /2 families involved in regulation of cell cycle progression. TaCRK2 is also located to the parasite nuclei but has no nuclear localization signal and exhibits transient up-regulation in protein levels during mid-merogony. However compared to TaCRK3, it shows down-regulation near the end of merogony. We predict that TaCRK3 may have a role in regulation of gene transcription while TaCRK2 is more likely to be involved in control of parasite nuclear division.

KW - Apicomplexa

KW - Cyclin-dependent kinases

KW - Differentiation

KW - Nuclear localization

KW - Theileria

UR - http://www.scopus.com/inward/record.url?scp=0035965775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035965775&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(01)00753-3

DO - 10.1016/S0378-1119(01)00753-3

M3 - Article

C2 - 11733137

AN - SCOPUS:0035965775

VL - 279

SP - 127

EP - 135

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -