Abstract
The TaCRK3 gene from the bovine apicomplexan parasite Theileria annulata, encodes a 46 kDa polypeptide with strong homology to the eukaryotic family of cyclin-dependent kinases. TaCRK3 does not show significant alignment with any particular CDK group, other than the Pfmrk kinases from the related apicomplexans Plasmodium falciparum and Plasmodium yoelii. It has a putative bipartite nuclear localization signal and is located to parasite nuclei by IFAT. Protein levels are constitutive throughout differentiation of the intra-lymphocytic macroschizont. This contrasts with the expression pattern of TaCRK2 (Kinnaird et al., 1996. Mol. Microbiol., 22, 293-302) which is closely related to the eukaryotic CDK1 /2 families involved in regulation of cell cycle progression. TaCRK2 is also located to the parasite nuclei but has no nuclear localization signal and exhibits transient up-regulation in protein levels during mid-merogony. However compared to TaCRK3, it shows down-regulation near the end of merogony. We predict that TaCRK3 may have a role in regulation of gene transcription while TaCRK2 is more likely to be involved in control of parasite nuclear division.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 127-135 |
| Number of pages | 9 |
| Journal | Gene |
| Volume | 279 |
| Issue number | 2 |
| DOIs | |
| State | Published - Nov 28 2001 |
| Externally published | Yes |
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Keywords
- Apicomplexa
- Cyclin-dependent kinases
- Differentiation
- Nuclear localization
- Theileria
ASJC Scopus subject areas
- Genetics
Cite this
TaCRK3 encodes a novel Theileria annulata protein kinase with motifs characteristic of the family of eukaryotic cyclin dependent kinases : A comparative analysis of its expression with TaCRK2 during the parasite life cycle. / Kinnaird, Jane; Logan, Mary; Tait, Andrew; Langsley, Gordon.
In: Gene, Vol. 279, No. 2, 28.11.2001, p. 127-135.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - TaCRK3 encodes a novel Theileria annulata protein kinase with motifs characteristic of the family of eukaryotic cyclin dependent kinases
T2 - A comparative analysis of its expression with TaCRK2 during the parasite life cycle
AU - Kinnaird, Jane
AU - Logan, Mary
AU - Tait, Andrew
AU - Langsley, Gordon
PY - 2001/11/28
Y1 - 2001/11/28
N2 - The TaCRK3 gene from the bovine apicomplexan parasite Theileria annulata, encodes a 46 kDa polypeptide with strong homology to the eukaryotic family of cyclin-dependent kinases. TaCRK3 does not show significant alignment with any particular CDK group, other than the Pfmrk kinases from the related apicomplexans Plasmodium falciparum and Plasmodium yoelii. It has a putative bipartite nuclear localization signal and is located to parasite nuclei by IFAT. Protein levels are constitutive throughout differentiation of the intra-lymphocytic macroschizont. This contrasts with the expression pattern of TaCRK2 (Kinnaird et al., 1996. Mol. Microbiol., 22, 293-302) which is closely related to the eukaryotic CDK1 /2 families involved in regulation of cell cycle progression. TaCRK2 is also located to the parasite nuclei but has no nuclear localization signal and exhibits transient up-regulation in protein levels during mid-merogony. However compared to TaCRK3, it shows down-regulation near the end of merogony. We predict that TaCRK3 may have a role in regulation of gene transcription while TaCRK2 is more likely to be involved in control of parasite nuclear division.
AB - The TaCRK3 gene from the bovine apicomplexan parasite Theileria annulata, encodes a 46 kDa polypeptide with strong homology to the eukaryotic family of cyclin-dependent kinases. TaCRK3 does not show significant alignment with any particular CDK group, other than the Pfmrk kinases from the related apicomplexans Plasmodium falciparum and Plasmodium yoelii. It has a putative bipartite nuclear localization signal and is located to parasite nuclei by IFAT. Protein levels are constitutive throughout differentiation of the intra-lymphocytic macroschizont. This contrasts with the expression pattern of TaCRK2 (Kinnaird et al., 1996. Mol. Microbiol., 22, 293-302) which is closely related to the eukaryotic CDK1 /2 families involved in regulation of cell cycle progression. TaCRK2 is also located to the parasite nuclei but has no nuclear localization signal and exhibits transient up-regulation in protein levels during mid-merogony. However compared to TaCRK3, it shows down-regulation near the end of merogony. We predict that TaCRK3 may have a role in regulation of gene transcription while TaCRK2 is more likely to be involved in control of parasite nuclear division.
KW - Apicomplexa
KW - Cyclin-dependent kinases
KW - Differentiation
KW - Nuclear localization
KW - Theileria
UR - http://www.scopus.com/inward/record.url?scp=0035965775&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035965775&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(01)00753-3
DO - 10.1016/S0378-1119(01)00753-3
M3 - Article
C2 - 11733137
AN - SCOPUS:0035965775
VL - 279
SP - 127
EP - 135
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -