T lymphocyte expression of complement receptor 2 (CR2/CD21): A role in adhesive cell-cell interactions and dysregulation in a patient with systemic lupus erythematosus (SLE)

E. Levy, J. Ambrus, Leslie Kahl, H. Molina, K. Tung, V. M. Holers

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Complement receptor 2 (CR2, CD21), the receptor for both the C3d,g portion of human complement component C3 and the Epstein-Barr virus, has been recently described on peripheral T cells. By using dual stain flow cytometric analysis, we have also observed that a peripheral T lymphocyte subpopulation of normal healthy donors bears CR2 in a range varying from 1.1 to 23.2% (mean 12.6%) of total CD3+ cells. T lymphocytes from nine patients with inactive SLE expressed CR2 in a similar range. Three patients with active SLE were also studied. One of them, having neuropathy and glomerulonephritis, displayed an expansion of the CR2+ T cell subpopulation which reached as much as 89% of total CD3+ cells. To examine potential functional roles of T cell CR2, cells from a Jurkat-derived CR2 expressing T cell line were found to bind in vitro to human CR2-, complement-coated K562 cell targets in a CR2- and complement-dependent fashion. Based on these studies, we hypothesize that CR2 might act to increase adherence of T cells to nucleated target cells bearing C3d,g, a function which may be relevant to cytotoxicity or other T cell activities requiring cell-cell interaction.

Original languageEnglish (US)
Pages (from-to)235-244
Number of pages10
JournalClinical and Experimental Immunology
Volume90
Issue number2
StatePublished - 1992
Externally publishedYes

Fingerprint

Complement 3d Receptors
Cell Communication
Systemic Lupus Erythematosus
Adhesives
T-Lymphocytes
Complement C3
Jurkat Cells
K562 Cells
Lymphocyte Subsets
Glomerulonephritis
Human Herpesvirus 4
Coloring Agents
Tissue Donors
Cell Line

Keywords

  • cell adhesion
  • CR2
  • SLE
  • T lymphocyte

ASJC Scopus subject areas

  • Immunology

Cite this

T lymphocyte expression of complement receptor 2 (CR2/CD21) : A role in adhesive cell-cell interactions and dysregulation in a patient with systemic lupus erythematosus (SLE). / Levy, E.; Ambrus, J.; Kahl, Leslie; Molina, H.; Tung, K.; Holers, V. M.

In: Clinical and Experimental Immunology, Vol. 90, No. 2, 1992, p. 235-244.

Research output: Contribution to journalArticle

@article{38a372c55d944ec89d68bec66ae5dd2b,
title = "T lymphocyte expression of complement receptor 2 (CR2/CD21): A role in adhesive cell-cell interactions and dysregulation in a patient with systemic lupus erythematosus (SLE)",
abstract = "Complement receptor 2 (CR2, CD21), the receptor for both the C3d,g portion of human complement component C3 and the Epstein-Barr virus, has been recently described on peripheral T cells. By using dual stain flow cytometric analysis, we have also observed that a peripheral T lymphocyte subpopulation of normal healthy donors bears CR2 in a range varying from 1.1 to 23.2{\%} (mean 12.6{\%}) of total CD3+ cells. T lymphocytes from nine patients with inactive SLE expressed CR2 in a similar range. Three patients with active SLE were also studied. One of them, having neuropathy and glomerulonephritis, displayed an expansion of the CR2+ T cell subpopulation which reached as much as 89{\%} of total CD3+ cells. To examine potential functional roles of T cell CR2, cells from a Jurkat-derived CR2 expressing T cell line were found to bind in vitro to human CR2-, complement-coated K562 cell targets in a CR2- and complement-dependent fashion. Based on these studies, we hypothesize that CR2 might act to increase adherence of T cells to nucleated target cells bearing C3d,g, a function which may be relevant to cytotoxicity or other T cell activities requiring cell-cell interaction.",
keywords = "cell adhesion, CR2, SLE, T lymphocyte",
author = "E. Levy and J. Ambrus and Leslie Kahl and H. Molina and K. Tung and Holers, {V. M.}",
year = "1992",
language = "English (US)",
volume = "90",
pages = "235--244",
journal = "Clinical and Experimental Immunology",
issn = "0009-9104",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - T lymphocyte expression of complement receptor 2 (CR2/CD21)

T2 - A role in adhesive cell-cell interactions and dysregulation in a patient with systemic lupus erythematosus (SLE)

AU - Levy, E.

AU - Ambrus, J.

AU - Kahl, Leslie

AU - Molina, H.

AU - Tung, K.

AU - Holers, V. M.

PY - 1992

Y1 - 1992

N2 - Complement receptor 2 (CR2, CD21), the receptor for both the C3d,g portion of human complement component C3 and the Epstein-Barr virus, has been recently described on peripheral T cells. By using dual stain flow cytometric analysis, we have also observed that a peripheral T lymphocyte subpopulation of normal healthy donors bears CR2 in a range varying from 1.1 to 23.2% (mean 12.6%) of total CD3+ cells. T lymphocytes from nine patients with inactive SLE expressed CR2 in a similar range. Three patients with active SLE were also studied. One of them, having neuropathy and glomerulonephritis, displayed an expansion of the CR2+ T cell subpopulation which reached as much as 89% of total CD3+ cells. To examine potential functional roles of T cell CR2, cells from a Jurkat-derived CR2 expressing T cell line were found to bind in vitro to human CR2-, complement-coated K562 cell targets in a CR2- and complement-dependent fashion. Based on these studies, we hypothesize that CR2 might act to increase adherence of T cells to nucleated target cells bearing C3d,g, a function which may be relevant to cytotoxicity or other T cell activities requiring cell-cell interaction.

AB - Complement receptor 2 (CR2, CD21), the receptor for both the C3d,g portion of human complement component C3 and the Epstein-Barr virus, has been recently described on peripheral T cells. By using dual stain flow cytometric analysis, we have also observed that a peripheral T lymphocyte subpopulation of normal healthy donors bears CR2 in a range varying from 1.1 to 23.2% (mean 12.6%) of total CD3+ cells. T lymphocytes from nine patients with inactive SLE expressed CR2 in a similar range. Three patients with active SLE were also studied. One of them, having neuropathy and glomerulonephritis, displayed an expansion of the CR2+ T cell subpopulation which reached as much as 89% of total CD3+ cells. To examine potential functional roles of T cell CR2, cells from a Jurkat-derived CR2 expressing T cell line were found to bind in vitro to human CR2-, complement-coated K562 cell targets in a CR2- and complement-dependent fashion. Based on these studies, we hypothesize that CR2 might act to increase adherence of T cells to nucleated target cells bearing C3d,g, a function which may be relevant to cytotoxicity or other T cell activities requiring cell-cell interaction.

KW - cell adhesion

KW - CR2

KW - SLE

KW - T lymphocyte

UR - http://www.scopus.com/inward/record.url?scp=0026480851&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026480851&partnerID=8YFLogxK

M3 - Article

C2 - 1424280

AN - SCOPUS:0026480851

VL - 90

SP - 235

EP - 244

JO - Clinical and Experimental Immunology

JF - Clinical and Experimental Immunology

SN - 0009-9104

IS - 2

ER -