TY - JOUR
T1 - T lymphocyte expression of complement receptor 2 (CR2/CD21)
T2 - A role in adhesive cell-cell interactions and dysregulation in a patient with systemic lupus erythematosus (SLE)
AU - Levy, E.
AU - Ambrus, J.
AU - Kahl, L.
AU - Molina, H.
AU - Tung, K.
AU - Holers, V. M.
PY - 1992
Y1 - 1992
N2 - Complement receptor 2 (CR2, CD21), the receptor for both the C3d,g portion of human complement component C3 and the Epstein-Barr virus, has been recently described on peripheral T cells. By using dual stain flow cytometric analysis, we have also observed that a peripheral T lymphocyte subpopulation of normal healthy donors bears CR2 in a range varying from 1.1 to 23.2% (mean 12.6%) of total CD3+ cells. T lymphocytes from nine patients with inactive SLE expressed CR2 in a similar range. Three patients with active SLE were also studied. One of them, having neuropathy and glomerulonephritis, displayed an expansion of the CR2+ T cell subpopulation which reached as much as 89% of total CD3+ cells. To examine potential functional roles of T cell CR2, cells from a Jurkat-derived CR2 expressing T cell line were found to bind in vitro to human CR2-, complement-coated K562 cell targets in a CR2- and complement-dependent fashion. Based on these studies, we hypothesize that CR2 might act to increase adherence of T cells to nucleated target cells bearing C3d,g, a function which may be relevant to cytotoxicity or other T cell activities requiring cell-cell interaction.
AB - Complement receptor 2 (CR2, CD21), the receptor for both the C3d,g portion of human complement component C3 and the Epstein-Barr virus, has been recently described on peripheral T cells. By using dual stain flow cytometric analysis, we have also observed that a peripheral T lymphocyte subpopulation of normal healthy donors bears CR2 in a range varying from 1.1 to 23.2% (mean 12.6%) of total CD3+ cells. T lymphocytes from nine patients with inactive SLE expressed CR2 in a similar range. Three patients with active SLE were also studied. One of them, having neuropathy and glomerulonephritis, displayed an expansion of the CR2+ T cell subpopulation which reached as much as 89% of total CD3+ cells. To examine potential functional roles of T cell CR2, cells from a Jurkat-derived CR2 expressing T cell line were found to bind in vitro to human CR2-, complement-coated K562 cell targets in a CR2- and complement-dependent fashion. Based on these studies, we hypothesize that CR2 might act to increase adherence of T cells to nucleated target cells bearing C3d,g, a function which may be relevant to cytotoxicity or other T cell activities requiring cell-cell interaction.
KW - CR2
KW - SLE
KW - T lymphocyte
KW - cell adhesion
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U2 - 10.1111/j.1365-2249.1992.tb07935.x
DO - 10.1111/j.1365-2249.1992.tb07935.x
M3 - Article
C2 - 1424280
AN - SCOPUS:0026480851
SN - 0009-9104
VL - 90
SP - 235
EP - 244
JO - Clinical and Experimental Immunology
JF - Clinical and Experimental Immunology
IS - 2
ER -