T cells produce TRF in response to Con A and factors in T cell hybridoma supernatants

D. H. Raulet, T. Hunig, David Parker

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In recent experiments, a soluble factor (TRF) that mediates the differentiation of anti-immunoglobulin (Ig)-activated B cells to Ig-secreting cells has been identified. TRF works in concert with a growth factor, probably IL 2, in the induction of activated B cells. In previous studies, TRF was identified in culture supernatants of activated T cells and accessory cells, and thus the cellular source (T cell or accessory cell) of the factor was not determined. In the present studies, we succeeded in inducing the production of TRF by T cell populations from which accessory cells had been vigorously depleted. Lymph node cells were depleted of accessory cells by nylon wool adherence and anti-Ia and complement treatment; these cells were activated with Con A and a T cell hybridoma supernatant that contains IL 2. Supernatants from these activated T cell cultures supported the differentiation of anti-Ig-activated B cells to Ig secreting cells. These results show that T cells produce the differentiation factor, and further that they do so in response to ligand (Con A) plus a T cell-derived factor.

Original languageEnglish (US)
Pages (from-to)908-912
Number of pages5
JournalJournal of Immunology
Volume128
Issue number2
StatePublished - 1982
Externally publishedYes

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TCF Transcription Factors
Hybridomas
T-Lymphocytes
Antibody-Producing Cells
B-Lymphocytes
Interleukin-2
Wool
Nylons
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Cell Culture Techniques
Lymph Nodes
Ligands
Population

ASJC Scopus subject areas

  • Immunology

Cite this

T cells produce TRF in response to Con A and factors in T cell hybridoma supernatants. / Raulet, D. H.; Hunig, T.; Parker, David.

In: Journal of Immunology, Vol. 128, No. 2, 1982, p. 908-912.

Research output: Contribution to journalArticle

Raulet, DH, Hunig, T & Parker, D 1982, 'T cells produce TRF in response to Con A and factors in T cell hybridoma supernatants', Journal of Immunology, vol. 128, no. 2, pp. 908-912.
Raulet, D. H. ; Hunig, T. ; Parker, David. / T cells produce TRF in response to Con A and factors in T cell hybridoma supernatants. In: Journal of Immunology. 1982 ; Vol. 128, No. 2. pp. 908-912.
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