T cells and HIV-induced T cell syncytia exhibit the same motility cycle

Andrew Sylwester, D. Shutt, D. Wessels, J. T. Stapleton, J. Stites, R. C. Kennedy, D. R. Soll

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Ameboid cells ranging in complexity from Dictyostelium amebas to human polymorphonuclear leukocytes (PMNs) translocate in a cyclical fashion. Using computer-assisted motion analysis, we have analyzed the motility of human lymphocytes of the immortal SupT1 cell line and of a peripheral blood mononuclear cell population highly enriched for CDL4-positive cells (CD4-enriched PBMCs) on four substrates - plastic, dehydrated rat tail collagen, hydrated rat tail collagen, and bovine aortic endothelium. In addition, we have analyzed the motility on these substrates of syncytia induced by human immunodeficiency virus (HIV) in cultures of both cell types. It is demonstrated that both SupT1 cells and CD4-enriched PBMCs exhibit a motility cycle with a period of 1.6 min that is independent of substrate, independent of average cell velocity, and similar to the periods of translocating Dictyostelium amebas and PMNs. More surprisingly, it is demonstrated that HIV-induced SupT1 and PBMC syncytia with volumes 10 to 100 times those of single cells exhibit the same motility cycle as their single-cell progenitors. These observations support the generality of the motility cycle in animal cells and, for the first time, demonstrate that the cycle is independent of cell size.

Original languageEnglish (US)
Pages (from-to)643-650
Number of pages8
JournalJournal of Leukocyte Biology
Volume57
Issue number4
StatePublished - 1995
Externally publishedYes

Fingerprint

Giant Cells
HIV
T-Lymphocytes
Amoeba
Dictyostelium
Tail
Collagen
Cell Size
Plastics
Endothelium
Blood Cells
Neutrophils
Stem Cells
Cell Culture Techniques
Lymphocytes
Cell Line
Population

Keywords

  • CD4-positive T lymphocytes
  • Cell motility cycle
  • Computer-assisted motion analysis
  • HIV-induced syncytium

ASJC Scopus subject areas

  • Cell Biology

Cite this

Sylwester, A., Shutt, D., Wessels, D., Stapleton, J. T., Stites, J., Kennedy, R. C., & Soll, D. R. (1995). T cells and HIV-induced T cell syncytia exhibit the same motility cycle. Journal of Leukocyte Biology, 57(4), 643-650.

T cells and HIV-induced T cell syncytia exhibit the same motility cycle. / Sylwester, Andrew; Shutt, D.; Wessels, D.; Stapleton, J. T.; Stites, J.; Kennedy, R. C.; Soll, D. R.

In: Journal of Leukocyte Biology, Vol. 57, No. 4, 1995, p. 643-650.

Research output: Contribution to journalArticle

Sylwester, A, Shutt, D, Wessels, D, Stapleton, JT, Stites, J, Kennedy, RC & Soll, DR 1995, 'T cells and HIV-induced T cell syncytia exhibit the same motility cycle', Journal of Leukocyte Biology, vol. 57, no. 4, pp. 643-650.
Sylwester A, Shutt D, Wessels D, Stapleton JT, Stites J, Kennedy RC et al. T cells and HIV-induced T cell syncytia exhibit the same motility cycle. Journal of Leukocyte Biology. 1995;57(4):643-650.
Sylwester, Andrew ; Shutt, D. ; Wessels, D. ; Stapleton, J. T. ; Stites, J. ; Kennedy, R. C. ; Soll, D. R. / T cells and HIV-induced T cell syncytia exhibit the same motility cycle. In: Journal of Leukocyte Biology. 1995 ; Vol. 57, No. 4. pp. 643-650.
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