@article{c1a3b16a502e4824afb51a8e9e977739,
title = "T cell recognition of Mycobacterium tuberculosis peptides presented by HLA-E derived from infected human cells",
abstract = "HLA-E is a non-conventional MHC Class I molecule that has been recently demonstrated to present pathogen-derived ligands, resulting in the TCR-dependent activation of αβ CD8+ T cells. The goal of this study was to characterize the ligandome displayed by HLA-E following infection with Mycobacterium tuberculosis (Mtb) using an in-depth mass spectrometry approach. Here we identified 28 Mtb ligands derived from 13 different source proteins, including the Esx family of proteins. When tested for activity with CD8+ T cells isolated from sixteen donors, nine of the ligands elicited an IFN-γ response from at least one donor, with fourteen of 16 donors responding to the Rv0634A19-29 peptide. Further evaluation of this immunodominant peptide response confirmed HLA-E restriction and the presence of Rv0634A19-29-reactive CD8+ T cells in the peripheral blood of human donors. The identification of an Mtb HLA-E ligand that is commonly recognized may provide a target for a non-traditional vaccine strategy.",
author = "Curtis McMurtrey and Harriff, {Melanie J.} and Swarbrick, {Gwendolyn M.} and Amanda Duncan and Meghan Cansler and Megan Null and Wilfried Bardet and Jackson, {Kenneth W.} and Lewinsohn, {Deborah A.} and William Hildebrand and Lewinsohn, {David M.}",
note = "Funding Information: This work was supported in part by Career Development Award # IK2 CX000538 from the U.S. Department of Veterans Affairs Clinical Sciences Research and Development Program (MJH), Merit Award #I01 BX000533 from the U.S. Department of Veterans Affairs Biomedical Laboratory Research and Development Program (DML), AI048090 (NIH, DML), HHSN272200900053C (NIH, DML), HHSN266200400081C (NIH, DML). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. OHSU and Drs. David and Deborah Lewinsohn have financial interest in ViTi, a company that may have a commercial interest in the result of this research and technology. Gwendolyn Swarbrick, Megan Null, and Meghan Cansler receive salary support from ViTi. These potential individual and institutional conflicts of interest have been reviewed and managed by OHSU. ViTi had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the {\textquoteleft}author contributions{\textquoteright} section. This does not alter our adherence to PLOS ONE policies on sharing data and materials. Publisher Copyright: {\textcopyright} 2017, Public Library of Science. All rights reserved. This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.",
year = "2017",
month = nov,
doi = "10.1371/journal.pone.0188288",
language = "English (US)",
volume = "12",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",
}