T cell receptor Vβ gene usage in the recognition of myelin basic protein by cerebrospinal fluid- and blood-derived T cells from patients with multiple sclerosis

Y. K. Chou, A. C. Buenafe, R. Dedrick, W. J. Morrison, Dennis Bourdette, Ruth Whitham, J. Atherton, J. Lane, E. Spoor, G. A. Hashim, Halina Offner, Arthur Vandenbark

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Because of its proximity to the central nervous system, the cerebrospinal fluid (CSF) represents an important source of T cells that potentially could mediate putative autoimmune diseases such as multiple sclerosis (MS). To overcome the low CSF cellularity, we evaluated culture conditions that could expand CSF T cells, with a focus on the expression of T-cell receptor Vβ genes utilized by T cells specific for the potentially encephalitogenic autoantigen myelin basic protein (BP). Expansion of 'activated' CSF cells with IL-2/IL-4 plus accessory cells optimally retained BP-responsive T cells that over-expressed Vβ1, Vβ2, Vβ5, or Vβ18, compared to expansion using supernatants from PHA-stimulated blood cells, or anti-CD3 antibody that led to different V gene bias and rare reactivity to BP. Sequential evaluation of paired CSF and blood samples from a relapsing remitting MS patient indicated that BP-reactive T cells were present in CSF during the period of clinical activity, and the pattern of BP recognition in CSF was partially reflected in blood, even after CSF reactivity had dissipated during remission. Over- expressed Vβ genes were not always constant, however, since in three sequential evaluations of a chronic progressive MS patient, Vβ genes over- expressed in the first BP-reactive CSF switched to a different Vβ gene bias that was present in the second and third CSF samples. Blood samples reflected each pattern of CSF Vβ gene bias, but retained the initial bias for at least 4 months after its disappearance from CSF. These data indicate that selective expansion of IL-2/IL-4-responsive CSF cells favors growth of the BP-reactive subpopulation, and, in a limited number of patients studied, reflected clinical disease activity. In comparison, blood T cells provided a partial but longer lasting reflection of the CSF BP reactivity and Vβ gene bias.

Original languageEnglish (US)
Pages (from-to)169-181
Number of pages13
JournalJournal of Neuroscience Research
Volume37
Issue number2
StatePublished - 1994

Fingerprint

T-Cell Receptor Genes
Myelin Basic Protein
Multiple Sclerosis
Cerebrospinal Fluid
T-Lymphocytes
Genes
Cerebrospinal Fluid Proteins
Proteins
Interleukin-4
Interleukin-2
Blood Cells
Chronic Progressive Multiple Sclerosis
Relapsing-Remitting Multiple Sclerosis
Autoantigens
Autoimmune Diseases
Anti-Idiotypic Antibodies

Keywords

  • experimental autoimmune encephalomyelitis
  • human CSF T cells
  • multiple sclerosis
  • myelin basic protein
  • T cell receptor Vβ genes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

T cell receptor Vβ gene usage in the recognition of myelin basic protein by cerebrospinal fluid- and blood-derived T cells from patients with multiple sclerosis. / Chou, Y. K.; Buenafe, A. C.; Dedrick, R.; Morrison, W. J.; Bourdette, Dennis; Whitham, Ruth; Atherton, J.; Lane, J.; Spoor, E.; Hashim, G. A.; Offner, Halina; Vandenbark, Arthur.

In: Journal of Neuroscience Research, Vol. 37, No. 2, 1994, p. 169-181.

Research output: Contribution to journalArticle

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abstract = "Because of its proximity to the central nervous system, the cerebrospinal fluid (CSF) represents an important source of T cells that potentially could mediate putative autoimmune diseases such as multiple sclerosis (MS). To overcome the low CSF cellularity, we evaluated culture conditions that could expand CSF T cells, with a focus on the expression of T-cell receptor Vβ genes utilized by T cells specific for the potentially encephalitogenic autoantigen myelin basic protein (BP). Expansion of 'activated' CSF cells with IL-2/IL-4 plus accessory cells optimally retained BP-responsive T cells that over-expressed Vβ1, Vβ2, Vβ5, or Vβ18, compared to expansion using supernatants from PHA-stimulated blood cells, or anti-CD3 antibody that led to different V gene bias and rare reactivity to BP. Sequential evaluation of paired CSF and blood samples from a relapsing remitting MS patient indicated that BP-reactive T cells were present in CSF during the period of clinical activity, and the pattern of BP recognition in CSF was partially reflected in blood, even after CSF reactivity had dissipated during remission. Over- expressed Vβ genes were not always constant, however, since in three sequential evaluations of a chronic progressive MS patient, Vβ genes over- expressed in the first BP-reactive CSF switched to a different Vβ gene bias that was present in the second and third CSF samples. Blood samples reflected each pattern of CSF Vβ gene bias, but retained the initial bias for at least 4 months after its disappearance from CSF. These data indicate that selective expansion of IL-2/IL-4-responsive CSF cells favors growth of the BP-reactive subpopulation, and, in a limited number of patients studied, reflected clinical disease activity. In comparison, blood T cells provided a partial but longer lasting reflection of the CSF BP reactivity and Vβ gene bias.",
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AU - Buenafe, A. C.

AU - Dedrick, R.

AU - Morrison, W. J.

AU - Bourdette, Dennis

AU - Whitham, Ruth

AU - Atherton, J.

AU - Lane, J.

AU - Spoor, E.

AU - Hashim, G. A.

AU - Offner, Halina

AU - Vandenbark, Arthur

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