TY - JOUR
T1 - T cell lines selected with synthetic peptides are highly encephalitogenic in SJL/J mice
AU - Bourdette, D. N.
AU - Vandenbark, A. A.
AU - Hashim, G.
AU - Whitham, R. H.
AU - Offner, H.
N1 - Funding Information:
Acknowledgements. This work was supported by the Veterans Administration, the Medical Research Foundation of Oregon, NIH grants NS 23221, NS 234A.a., and NS 21466 and NSF grant BNS 11133. We thank Dr. C.-J. Chou for generously providing some of the materials used in this study. We thank Joe Turner and Alex Lin for their technical assistance, and Lynn Riley and Karin Coffee for secretarial assistance.
PY - 1989/5
Y1 - 1989/5
N2 - T cell lines were selected from basic protein (BP)-immunized SJL/J mice using synthetic peptides encompassing the major SJL/J encephalitogenic determinant. Synthetic peptide-derived T cell lines proliferated in response to BP, the 89-169 peptides fragment of BP and the synthetic peptides, pM87-99, pM90-99 and pM91-99. These lines transferred a demyelinating and chronic relapsing form of experimental autoimmune encephalomyelitis (EAE) into naive mice, and EAE induced by synthetic peptide-derived lines was more severe than that induced by whole BP-derived lines. This study demonstrates that T cell lines selected with synthetic peptides are encephalitogenic in SJL/J mice and offers an improved means for selecting SJL/J encephalitogenic T cell lines.
AB - T cell lines were selected from basic protein (BP)-immunized SJL/J mice using synthetic peptides encompassing the major SJL/J encephalitogenic determinant. Synthetic peptide-derived T cell lines proliferated in response to BP, the 89-169 peptides fragment of BP and the synthetic peptides, pM87-99, pM90-99 and pM91-99. These lines transferred a demyelinating and chronic relapsing form of experimental autoimmune encephalomyelitis (EAE) into naive mice, and EAE induced by synthetic peptide-derived lines was more severe than that induced by whole BP-derived lines. This study demonstrates that T cell lines selected with synthetic peptides are encephalitogenic in SJL/J mice and offers an improved means for selecting SJL/J encephalitogenic T cell lines.
KW - (SJL/J mouse)
KW - Basic protein-specific T cell line
KW - Encephalitogenic epitope
KW - Experimental autoimmune encephalomyelitis
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U2 - 10.1016/0165-5728(89)90023-4
DO - 10.1016/0165-5728(89)90023-4
M3 - Article
C2 - 2467920
AN - SCOPUS:0024514478
SN - 0165-5728
VL - 22
SP - 255
EP - 260
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 3
ER -