T cell lines selected with synthetic peptides are highly encephalitogenic in SJL/J mice

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Abstract

T cell lines were selected from basic protein (BP)-immunized SJL/J mice using synthetic peptides encompassing the major SJL/J encephalitogenic determinant. Synthetic peptide-derived T cell lines proliferated in response to BP, the 89-169 peptides fragment of BP and the synthetic peptides, pM87-99, pM90-99 and pM91-99. These lines transferred a demyelinating and chronic relapsing form of experimental autoimmune encephalomyelitis (EAE) into naive mice, and EAE induced by synthetic peptide-derived lines was more severe than that induced by whole BP-derived lines. This study demonstrates that T cell lines selected with synthetic peptides are encephalitogenic in SJL/J mice and offers an improved means for selecting SJL/J encephalitogenic T cell lines.

Original languageEnglish (US)
Pages (from-to)255-260
Number of pages6
JournalJournal of Neuroimmunology
Volume22
Issue number3
DOIs
StatePublished - 1989

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T-Lymphocytes
Cell Line
Peptides
Autoimmune Experimental Encephalomyelitis
Proteins
Peptide T
Peptide Fragments

Keywords

  • (SJL/J mouse)
  • Basic protein-specific T cell line
  • Encephalitogenic epitope
  • Experimental autoimmune encephalomyelitis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

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title = "T cell lines selected with synthetic peptides are highly encephalitogenic in SJL/J mice",
abstract = "T cell lines were selected from basic protein (BP)-immunized SJL/J mice using synthetic peptides encompassing the major SJL/J encephalitogenic determinant. Synthetic peptide-derived T cell lines proliferated in response to BP, the 89-169 peptides fragment of BP and the synthetic peptides, pM87-99, pM90-99 and pM91-99. These lines transferred a demyelinating and chronic relapsing form of experimental autoimmune encephalomyelitis (EAE) into naive mice, and EAE induced by synthetic peptide-derived lines was more severe than that induced by whole BP-derived lines. This study demonstrates that T cell lines selected with synthetic peptides are encephalitogenic in SJL/J mice and offers an improved means for selecting SJL/J encephalitogenic T cell lines.",
keywords = "(SJL/J mouse), Basic protein-specific T cell line, Encephalitogenic epitope, Experimental autoimmune encephalomyelitis",
author = "Dennis Bourdette and Arthur Vandenbark and G. Hashim and Ruth Whitham and Halina Offner",
year = "1989",
doi = "10.1016/0165-5728(89)90023-4",
language = "English (US)",
volume = "22",
pages = "255--260",
journal = "Journal of Neuroimmunology",
issn = "0165-5728",
publisher = "Elsevier",
number = "3",

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TY - JOUR

T1 - T cell lines selected with synthetic peptides are highly encephalitogenic in SJL/J mice

AU - Bourdette, Dennis

AU - Vandenbark, Arthur

AU - Hashim, G.

AU - Whitham, Ruth

AU - Offner, Halina

PY - 1989

Y1 - 1989

N2 - T cell lines were selected from basic protein (BP)-immunized SJL/J mice using synthetic peptides encompassing the major SJL/J encephalitogenic determinant. Synthetic peptide-derived T cell lines proliferated in response to BP, the 89-169 peptides fragment of BP and the synthetic peptides, pM87-99, pM90-99 and pM91-99. These lines transferred a demyelinating and chronic relapsing form of experimental autoimmune encephalomyelitis (EAE) into naive mice, and EAE induced by synthetic peptide-derived lines was more severe than that induced by whole BP-derived lines. This study demonstrates that T cell lines selected with synthetic peptides are encephalitogenic in SJL/J mice and offers an improved means for selecting SJL/J encephalitogenic T cell lines.

AB - T cell lines were selected from basic protein (BP)-immunized SJL/J mice using synthetic peptides encompassing the major SJL/J encephalitogenic determinant. Synthetic peptide-derived T cell lines proliferated in response to BP, the 89-169 peptides fragment of BP and the synthetic peptides, pM87-99, pM90-99 and pM91-99. These lines transferred a demyelinating and chronic relapsing form of experimental autoimmune encephalomyelitis (EAE) into naive mice, and EAE induced by synthetic peptide-derived lines was more severe than that induced by whole BP-derived lines. This study demonstrates that T cell lines selected with synthetic peptides are encephalitogenic in SJL/J mice and offers an improved means for selecting SJL/J encephalitogenic T cell lines.

KW - (SJL/J mouse)

KW - Basic protein-specific T cell line

KW - Encephalitogenic epitope

KW - Experimental autoimmune encephalomyelitis

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DO - 10.1016/0165-5728(89)90023-4

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AN - SCOPUS:0024514478

VL - 22

SP - 255

EP - 260

JO - Journal of Neuroimmunology

JF - Journal of Neuroimmunology

SN - 0165-5728

IS - 3

ER -