T-cell epitope mapping in mycobacterium tuberculosis using pepmixes created by micro-scale spot™-synthesis

Marisa Frieder, David Lewinsohn

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Citations (Scopus)

Abstract

Mycobacterium tuberculosis (Mtb) remains a major threat to human health worldwide. Although treatment of infection is an important part of tuberculosis control, an improved vaccine is essential for the elimination of this disease. Control of infection with Mtb is dependent on the cellular immune system, which in turn requires an understanding of those antigens that are capable of stimulating CD4+ and CD8+ T-cell responses. Peptide libraries provide a high-throughput system for identifying novel T-cell epitopes. They can also be used to assess the hierarchy of immunodominance of these novel antigens and epitopes that are associated with infection with Mtb. This T-cell-driven means of antigen discovery is well adapted to vaccine development as well as developing the tools necessary to understand the natural history of this important human pathogen.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages369-382
Number of pages14
Volume524
DOIs
StatePublished - 2009

Publication series

NameMethods in Molecular Biology
Volume524
ISSN (Print)10643745

Fingerprint

Epitope Mapping
T-Lymphocyte Epitopes
Mycobacterium tuberculosis
Antigens
Vaccines
Disease Eradication
T-Lymphocytes
Peptide Library
Infection Control
Infection
Natural History
Epitopes
Immune System
Tuberculosis

Keywords

  • Antigen/epitope discovery
  • CD8+ T cells
  • Elispot
  • Immunodominance
  • Peptide library
  • Tuberculosis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Frieder, M., & Lewinsohn, D. (2009). T-cell epitope mapping in mycobacterium tuberculosis using pepmixes created by micro-scale spot™-synthesis. In Methods in Molecular Biology (Vol. 524, pp. 369-382). (Methods in Molecular Biology; Vol. 524). https://doi.org/10.1007/978-1-59745-450-6_27

T-cell epitope mapping in mycobacterium tuberculosis using pepmixes created by micro-scale spot™-synthesis. / Frieder, Marisa; Lewinsohn, David.

Methods in Molecular Biology. Vol. 524 2009. p. 369-382 (Methods in Molecular Biology; Vol. 524).

Research output: Chapter in Book/Report/Conference proceedingChapter

Frieder, M & Lewinsohn, D 2009, T-cell epitope mapping in mycobacterium tuberculosis using pepmixes created by micro-scale spot™-synthesis. in Methods in Molecular Biology. vol. 524, Methods in Molecular Biology, vol. 524, pp. 369-382. https://doi.org/10.1007/978-1-59745-450-6_27
Frieder, Marisa ; Lewinsohn, David. / T-cell epitope mapping in mycobacterium tuberculosis using pepmixes created by micro-scale spot™-synthesis. Methods in Molecular Biology. Vol. 524 2009. pp. 369-382 (Methods in Molecular Biology).
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