T cell acquisition of APC membrane can impact interpretation of adoptive transfer experiments using CD45 congenic mouse strains

Kathy S. Cho, Ann B. Hill

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Congenic mouse strains bearing allelic variants of CD45 are often used in adoptive transfer experiments. Here, we report that immune CD8+ splenocytes of donor origin acquired the recipient's congenic CD45 marker during interaction with antigen-bearing cells, presumably as a result of membrane transfer upon dissolution of the immunological synapse. Acquisition of recipient marker by donor cells was most prominent after in vitro incubation with peptide for intracellular cytokine staining, where most of the antigen-bearing splenocytes are of recipient origin. In consequence, when antibodies against the recipient's congenic marker were used to distinguish donor and recipient populations, donor origin cells were incorrectly interpreted as being of recipient origin. This phenomenon may cause problems for interpretation of data in adoptive transfer experiments primarily when (a) staining for the recipient's congenic marker and (b) identifying antigen-specific populations by staining for intracellular cytokine.

Original languageEnglish (US)
Pages (from-to)137-145
Number of pages9
JournalJournal of Immunological Methods
Volume330
Issue number1-2
DOIs
StatePublished - Jan 31 2008

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Keywords

  • Adoptive transfer
  • CD45 congenic
  • Membrane transfer
  • Murine cytomegalovirus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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