T- and B-cell-deficient mice with experimental stroke have reduced lesion size and inflammation

Patricia D. Hurn, Sandhya Subramanian, Susan M. Parker, Michael E. Afentoulis, Laurie J. Kaler, Arthur Vandenbark, Halina Offner

Research output: Contribution to journalArticle

236 Citations (Scopus)

Abstract

Stroke induction in immunologically competent mice not only produces local ischemia and brain damage, but also induces early inflammatory changes in brain and peripheral immune responses. Although immune elements clearly are activated after brain vascular occlusion, the relative contribution of T and B lymphocytes to the developing lesion has not been quantified. We evaluated effects 22 h after middle cerebral artery occlusion (90 mins) on histologic injury and peripheral immune activation in severe combined immunodeficient (SCID) mice lacking T and B cells. Cortical and total infarct volumes were strikingly reduced in male SCID mice (n=14, 33±4% of contralateral cortex, n=10, 52±3% of contralateral hemisphere) versus immunologically intact C57BL/6 mice (wild type, n=9, 57±5% of contralateral cortex, 57±4% of contralateral hemisphere) (P

Original languageEnglish (US)
Pages (from-to)1798-1805
Number of pages8
JournalJournal of Cerebral Blood Flow and Metabolism
Volume27
Issue number11
DOIs
StatePublished - Nov 2007

Fingerprint

SCID Mice
B-Lymphocytes
Stroke
Inflammation
T-Lymphocytes
Middle Cerebral Artery Infarction
Brain
Brain Ischemia
Inbred C57BL Mouse
Blood Vessels
Wounds and Injuries

Keywords

  • Cerebral ischemia
  • Chemokines
  • Cytokines
  • Spleen
  • T/B-deficient mice

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

T- and B-cell-deficient mice with experimental stroke have reduced lesion size and inflammation. / Hurn, Patricia D.; Subramanian, Sandhya; Parker, Susan M.; Afentoulis, Michael E.; Kaler, Laurie J.; Vandenbark, Arthur; Offner, Halina.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 27, No. 11, 11.2007, p. 1798-1805.

Research output: Contribution to journalArticle

Hurn, Patricia D. ; Subramanian, Sandhya ; Parker, Susan M. ; Afentoulis, Michael E. ; Kaler, Laurie J. ; Vandenbark, Arthur ; Offner, Halina. / T- and B-cell-deficient mice with experimental stroke have reduced lesion size and inflammation. In: Journal of Cerebral Blood Flow and Metabolism. 2007 ; Vol. 27, No. 11. pp. 1798-1805.
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