Synaptic Specializations Support Frequency-Independent Purkinje Cell Output from the Cerebellar Cortex

Josef Turecek, Skyler Jackman, Wade G. Regehr

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The output of the cerebellar cortex is conveyed to the deep cerebellar nuclei (DCN) by Purkinje cells (PCs). Here, we characterize the properties of the PC-DCN synapse in juvenile and adult mice and find that prolonged high-frequency stimulation leads to steady-state responses that become increasingly frequency independent within the physiological firing range of PCs in older animals, resulting in a linear relationship between charge transfer and activation frequency. We used a low-affinity antagonist to show that GABAA-receptor saturation occurs at this synapse but does not underlie frequency-invariant transmission. We propose that PC-DCN synapses have two components of release: one prominent early in trains and another specialized to maintain transmission during prolonged activation. Short-term facilitation offsets partial vesicle depletion to produce frequency-independent transmission.

Original languageEnglish (US)
Pages (from-to)3256-3268
Number of pages13
JournalCell Reports
Volume17
Issue number12
DOIs
StatePublished - Dec 20 2016
Externally publishedYes

Fingerprint

Cerebellar Cortex
Purkinje Cells
Cerebellar Nuclei
Synapses
Chemical activation
GABA-A Receptors
Charge transfer
Animals

Keywords

  • cerebellum
  • deep cerebellar nucleus
  • presynaptic
  • Purkinje cells
  • receptor saturation
  • recovery from depression
  • short-term facilitation
  • TPMPA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Synaptic Specializations Support Frequency-Independent Purkinje Cell Output from the Cerebellar Cortex. / Turecek, Josef; Jackman, Skyler; Regehr, Wade G.

In: Cell Reports, Vol. 17, No. 12, 20.12.2016, p. 3256-3268.

Research output: Contribution to journalArticle

@article{c8ec409527f7482e9e9b77af0075568f,
title = "Synaptic Specializations Support Frequency-Independent Purkinje Cell Output from the Cerebellar Cortex",
abstract = "The output of the cerebellar cortex is conveyed to the deep cerebellar nuclei (DCN) by Purkinje cells (PCs). Here, we characterize the properties of the PC-DCN synapse in juvenile and adult mice and find that prolonged high-frequency stimulation leads to steady-state responses that become increasingly frequency independent within the physiological firing range of PCs in older animals, resulting in a linear relationship between charge transfer and activation frequency. We used a low-affinity antagonist to show that GABAA-receptor saturation occurs at this synapse but does not underlie frequency-invariant transmission. We propose that PC-DCN synapses have two components of release: one prominent early in trains and another specialized to maintain transmission during prolonged activation. Short-term facilitation offsets partial vesicle depletion to produce frequency-independent transmission.",
keywords = "cerebellum, deep cerebellar nucleus, presynaptic, Purkinje cells, receptor saturation, recovery from depression, short-term facilitation, TPMPA",
author = "Josef Turecek and Skyler Jackman and Regehr, {Wade G.}",
year = "2016",
month = "12",
day = "20",
doi = "10.1016/j.celrep.2016.11.081",
language = "English (US)",
volume = "17",
pages = "3256--3268",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "12",

}

TY - JOUR

T1 - Synaptic Specializations Support Frequency-Independent Purkinje Cell Output from the Cerebellar Cortex

AU - Turecek, Josef

AU - Jackman, Skyler

AU - Regehr, Wade G.

PY - 2016/12/20

Y1 - 2016/12/20

N2 - The output of the cerebellar cortex is conveyed to the deep cerebellar nuclei (DCN) by Purkinje cells (PCs). Here, we characterize the properties of the PC-DCN synapse in juvenile and adult mice and find that prolonged high-frequency stimulation leads to steady-state responses that become increasingly frequency independent within the physiological firing range of PCs in older animals, resulting in a linear relationship between charge transfer and activation frequency. We used a low-affinity antagonist to show that GABAA-receptor saturation occurs at this synapse but does not underlie frequency-invariant transmission. We propose that PC-DCN synapses have two components of release: one prominent early in trains and another specialized to maintain transmission during prolonged activation. Short-term facilitation offsets partial vesicle depletion to produce frequency-independent transmission.

AB - The output of the cerebellar cortex is conveyed to the deep cerebellar nuclei (DCN) by Purkinje cells (PCs). Here, we characterize the properties of the PC-DCN synapse in juvenile and adult mice and find that prolonged high-frequency stimulation leads to steady-state responses that become increasingly frequency independent within the physiological firing range of PCs in older animals, resulting in a linear relationship between charge transfer and activation frequency. We used a low-affinity antagonist to show that GABAA-receptor saturation occurs at this synapse but does not underlie frequency-invariant transmission. We propose that PC-DCN synapses have two components of release: one prominent early in trains and another specialized to maintain transmission during prolonged activation. Short-term facilitation offsets partial vesicle depletion to produce frequency-independent transmission.

KW - cerebellum

KW - deep cerebellar nucleus

KW - presynaptic

KW - Purkinje cells

KW - receptor saturation

KW - recovery from depression

KW - short-term facilitation

KW - TPMPA

UR - http://www.scopus.com/inward/record.url?scp=85006757653&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85006757653&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2016.11.081

DO - 10.1016/j.celrep.2016.11.081

M3 - Article

VL - 17

SP - 3256

EP - 3268

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 12

ER -