Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation

Delphine Malherbe, Veit J. Erpenbeck, Soman N. Abraham, Erika C. Crouch, Jens M. Hohlfeld, Jo Rae Wright

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Mast cells play a key role in allergy and asthma. They reside at the host-environment interface and are among the first cells to make contact with inhaled microorganisms and particulate antigens. Pulmonary surfactant proteins A and D (SP-A and SP-D) function in lung host defense by enhancing microbe phagocytosis and mediating other immune cell functions, but little is known about their effects on mast cells. We hypothesized that SP-A and/or SP-D modulate IgE-dependent mast cell functions. Pollen starch granules (PSG) extracted from Dactylis glomerata and coated with trinitrophenol (TNP) were used as a model of an inhaled organic particulate allergen. Our data revealed that SP-D inhibited by 50% the release of β-hexosaminidase by peritoneal mast cells sensitized with IgE anti-TNP and stimulated with TNP-PSG. In contrast, SP-A had no effect. Furthermore, SP-D aggregated PSG in a dose-dependent manner, and this aggregation was mediated by SP-D's carbohydrate recognition domain. A single arm SP-D mutant (RrSP-Dser15,20) neither aggregated PSG nor inhibited degranulation, suggesting that multimerization of SP-D is required for maximal PSG aggregation and inhibition of PSG-induced mast cell degranulation. This study is the first to demonstrate that SP-D modulates IgE-mediated mast cell functions, which are important in asthma and allergic inflammation.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume289
Issue number5 33-5
DOIs
StatePublished - Nov 2005
Externally publishedYes

Fingerprint

Pulmonary Surfactant-Associated Protein D
Cell Degranulation
Pollen
Mast Cells
Immunoglobulin E
Starch
Asthma
Dactylis
Pulmonary Surfactant-Associated Protein A
Hexosaminidases
Phagocytosis
Allergens
Hypersensitivity
Carbohydrates
Inflammation
Antigens
Lung

Keywords

  • Allergen
  • Collectins
  • Immunoglobulin E
  • Innate immunity
  • Lung

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology

Cite this

Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation. / Malherbe, Delphine; Erpenbeck, Veit J.; Abraham, Soman N.; Crouch, Erika C.; Hohlfeld, Jens M.; Wright, Jo Rae.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 289, No. 5 33-5, 11.2005.

Research output: Contribution to journalArticle

Malherbe, Delphine ; Erpenbeck, Veit J. ; Abraham, Soman N. ; Crouch, Erika C. ; Hohlfeld, Jens M. ; Wright, Jo Rae. / Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2005 ; Vol. 289, No. 5 33-5.
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