Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation

Delphine C. Malherbe, Veit J. Erpenbeck, Soman N. Abraham, Erika C. Crouch, Jens M. Hohlfeld, Jo Rae Wright

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    26 Scopus citations

    Abstract

    Mast cells play a key role in allergy and asthma. They reside at the host-environment interface and are among the first cells to make contact with inhaled microorganisms and particulate antigens. Pulmonary surfactant proteins A and D (SP-A and SP-D) function in lung host defense by enhancing microbe phagocytosis and mediating other immune cell functions, but little is known about their effects on mast cells. We hypothesized that SP-A and/or SP-D modulate IgE-dependent mast cell functions. Pollen starch granules (PSG) extracted from Dactylis glomerata and coated with trinitrophenol (TNP) were used as a model of an inhaled organic particulate allergen. Our data revealed that SP-D inhibited by 50% the release of β-hexosaminidase by peritoneal mast cells sensitized with IgE anti-TNP and stimulated with TNP-PSG. In contrast, SP-A had no effect. Furthermore, SP-D aggregated PSG in a dose-dependent manner, and this aggregation was mediated by SP-D's carbohydrate recognition domain. A single arm SP-D mutant (RrSP-Dser15,20) neither aggregated PSG nor inhibited degranulation, suggesting that multimerization of SP-D is required for maximal PSG aggregation and inhibition of PSG-induced mast cell degranulation. This study is the first to demonstrate that SP-D modulates IgE-mediated mast cell functions, which are important in asthma and allergic inflammation.

    Original languageEnglish (US)
    Pages (from-to)L856-L866
    JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
    Volume289
    Issue number5 33-5
    DOIs
    StatePublished - Nov 1 2005

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    Keywords

    • Allergen
    • Collectins
    • Immunoglobulin E
    • Innate immunity
    • Lung

    ASJC Scopus subject areas

    • Physiology
    • Pulmonary and Respiratory Medicine
    • Physiology (medical)
    • Cell Biology

    Cite this

    Malherbe, D. C., Erpenbeck, V. J., Abraham, S. N., Crouch, E. C., Hohlfeld, J. M., & Wright, J. R. (2005). Surfactant protein D decreases pollen-induced IgE-dependent mast cell degranulation. American Journal of Physiology - Lung Cellular and Molecular Physiology, 289(5 33-5), L856-L866. https://doi.org/10.1152/ajplung.00009.2005