Suppression of proteoglycan synthesis in chondrocyte cultures derived from canine intervertebral disc

Jung U. Yoo, Roberts Papay, Charles J. Malemud

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Chondrocytes were dissociated enzymatically from canine nucleus pulposus. After attaining monolayer growth, the cells were subpassaged and incubated with sodium salicylate, indomethacin, or sodium meclofenamate (10-2 M to 3 x 10˜6 M). When incubated with nonsteroidal antiinflammatory drug concentrations mimicking therapeutic plasma levels, proteoglycan synthesis was significantly suppressed. After 24 hours of incubation, there was a 15% decrease in radioactive sulfate (35S04) incorporation for salicylate (10-3 M), a 29% de¬crease for indomethacin (3 x 10-6 M), and a 75% decrease for sodium meclofenamate (10“5 M). This inhibition was both dose and timedependent. Despite the effect of these nonsteroidal anti-inflammatory drugs on quantitative proteoglycan synthesis, there was no demonstrable effect of these drugs on the size of proteoglycan monomers or the degree to which proteoglycan aggregate formation occurred.

Original languageEnglish (US)
Pages (from-to)221-224
Number of pages4
JournalSpine
Volume17
Issue number2
DOIs
StatePublished - Feb 1992

Keywords

  • Nonsteroidal anti-inflammatory drugs
  • Nucleus pulposus chondrocytes
  • Proteoglycan synthesis

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Clinical Neurology

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