Chondrocytes were dissociated enzymatically from canine nucleus pulposus. After attaining monolayer growth, the cells were subpassaged and incubated with sodium salicylate, indomethacin, or sodium meclofenamate (10-2 M to 3 x 10˜6 M). When incubated with nonsteroidal antiinflammatory drug concentrations mimicking therapeutic plasma levels, proteoglycan synthesis was significantly suppressed. After 24 hours of incubation, there was a 15% decrease in radioactive sulfate (35S04) incorporation for salicylate (10-3 M), a 29% de¬crease for indomethacin (3 x 10-6 M), and a 75% decrease for sodium meclofenamate (10“5 M). This inhibition was both dose and timedependent. Despite the effect of these nonsteroidal anti-inflammatory drugs on quantitative proteoglycan synthesis, there was no demonstrable effect of these drugs on the size of proteoglycan monomers or the degree to which proteoglycan aggregate formation occurred.
- Nonsteroidal anti-inflammatory drugs
- Nucleus pulposus chondrocytes
- Proteoglycan synthesis
ASJC Scopus subject areas
- Orthopedics and Sports Medicine
- Clinical Neurology