[3H]Methotrexate Loss from the Rat Brain following Enhanced Uptake by Osmotic Opening of the Blood-Brain Barrier

Masahiro Ohata, Wendy R. Fredericks, Edward A. Neuwelt, Uma Sundaram, Stanley I. Rapoport, Masahiro Ohata, Wendy R. Fredericks, Edward A. Neuwelt, Uma Sundaram, Stanley I. Rapoport

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Abstract

Right brain regions of anesthetized rats were loaded with [3,5,7-3H]methotrexate (3H]MTX) or with [14C]sucrose by infusing the tracers into the right carotid artery, after the blood-brain barrier had been opened by right carotid infusion of a hypertonic arabinose solution. During the 6 hr following the procedure, the [3H]MTX concentration in 7 right-sided brain regions, when normalized to the plasma concentration integral during tracer infusion, fell, with an average half-time of 4.8 hr as compared to less than 20 min for the initial rate of toss of [14C]sucrose. Right-left brain concentration differences 3 hr after treatment were statistically significant (p < 0.05) for [3H]MTX but not for [14C]sucrose. The results indicate that intracerebral [3H]MTX is lost more slowly than is intracerebral [14C]sucrose, possibly because [3H]MTX enters brain cells, whereas [14C]sucrose remains largely extracellular.

Original languageEnglish (US)
Pages (from-to)1092-1096
Number of pages5
JournalCancer Research
Volume45
Issue number3
StatePublished - Mar 1 1985

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Ohata, M., Fredericks, W. R., Neuwelt, E. A., Sundaram, U., Rapoport, S. I., Ohata, M., Fredericks, W. R., Neuwelt, E. A., Sundaram, U., & Rapoport, S. I. (1985). [3H]Methotrexate Loss from the Rat Brain following Enhanced Uptake by Osmotic Opening of the Blood-Brain Barrier. Cancer Research, 45(3), 1092-1096.