TY - JOUR
T1 - 31P and 1H magnetic resonance spectroscopy of acute alcohol cardiac depression in rats
AU - Auffermann, Wolfgang
AU - Camacho, S. Albert
AU - Wu, Shao
AU - Litt, Lawrence
AU - Parmley, William W.
AU - Higgins, Charles B.
AU - Wikman‐Coffelt, Joan
PY - 1988/9
Y1 - 1988/9
N2 - Cardiac depression in the isolated rat heart perfused with 4% ethanol was correlated with intracellular phosphate energetics and tissue water distributions. Energy metabolites were assessed using 31P magnetic resonance spectroscopy (MRS) and correlated to the mitochondrial redox state using epicardial surface fluorometry. Changes in myocardial water compartmentation were measured by using 1H NMR spectroscopy with an extracellular chemical‐shift reagent (DyTTHA) and correlated to results of 2D echocardiography (2DE). During alcohol perfusion there was a significant decrease in developed pressure and in coronary flow. No change was seen in ATP, PCr, pHi, Pi, or NADH. After withdrawal of alcohol from the perfusate cardiac function reverted to control values without a depletion of energy levels. During alcohol perfusion 'H MRS showed a marked redistribution of water from the intra‐ to the extracellular space, corresponding to a 35% left ventricular wall thinning confirmed by 2DE. The results indicate that acute alcohol cardiac depression is related to a dehydration of myocardial cells, but is not associated with intracellular acidosis or energy depletion. 0 1988 Academic Press, Inc. © 1988 Academic Press, Inc.
AB - Cardiac depression in the isolated rat heart perfused with 4% ethanol was correlated with intracellular phosphate energetics and tissue water distributions. Energy metabolites were assessed using 31P magnetic resonance spectroscopy (MRS) and correlated to the mitochondrial redox state using epicardial surface fluorometry. Changes in myocardial water compartmentation were measured by using 1H NMR spectroscopy with an extracellular chemical‐shift reagent (DyTTHA) and correlated to results of 2D echocardiography (2DE). During alcohol perfusion there was a significant decrease in developed pressure and in coronary flow. No change was seen in ATP, PCr, pHi, Pi, or NADH. After withdrawal of alcohol from the perfusate cardiac function reverted to control values without a depletion of energy levels. During alcohol perfusion 'H MRS showed a marked redistribution of water from the intra‐ to the extracellular space, corresponding to a 35% left ventricular wall thinning confirmed by 2DE. The results indicate that acute alcohol cardiac depression is related to a dehydration of myocardial cells, but is not associated with intracellular acidosis or energy depletion. 0 1988 Academic Press, Inc. © 1988 Academic Press, Inc.
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U2 - 10.1002/mrm.1910080107
DO - 10.1002/mrm.1910080107
M3 - Article
C2 - 3173069
AN - SCOPUS:0023819362
SN - 0740-3194
VL - 8
SP - 58
EP - 69
JO - Magnetic Resonance in Medicine
JF - Magnetic Resonance in Medicine
IS - 1
ER -