Styrene-maleic acid copolymer effects on the function of the GPCR rhodopsin in lipid nanoparticles

Istvan Szundi, Stephanie G. Pitch, Eefei Chen, David L. Farrens, David S. Kliger

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Styrene-maleic acid (SMA) copolymers solubilize biological membranes to form lipid nanoparticles (SMALPs) that contain membrane proteins surrounded by native lipids, thus enabling the use of a variety of biophysical techniques for structural and functional studies. The question of whether SMALPs provide a truly natural environment or SMA solubilization affects the functional properties of membrane proteins, however, remains open. We address this question by comparing the photoactivation kinetics of rhodopsin, a G-protein-coupled receptor in the disk membranes of rod cells, in native membrane and SMALPs prepared at different molar ratios between SMA(3:1) and rhodopsin. Time-resolved absorption spectroscopy combined with complex kinetic analysis reveals kinetic and mechanistic differences between the native membrane and SMA-stabilized environment. The results suggest a range of molar ratios for nanoparticles suitable for kinetic studies.

Original languageEnglish (US)
Pages (from-to)4337-4348
Number of pages12
JournalBiophysical Journal
Volume120
Issue number20
DOIs
StatePublished - Oct 19 2021
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics

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