Post-transcriptional regulation of mRNA can potently dictate protein expression patterns in eukaryotic cells. This mode of regulation occurs through cis -acting regulatory regions in the mRNA transcript that mediate direct interactions with trans -acting RNA-binding proteins (RBPs). This mRNA/protein interaction can be studied in numerous ways that range from in vitro to in vivo through messenger ribonucleoprotein immunoprecipitation (mRNP-IP or RIP) assays. This modified immunoprecipitation approach is an important and sensitive method to determine the regulation of gene expression by specific RBPs under different cellular stressors.
- Post-transcriptional gene regulation
- RNA-binding proteins
- Ribonucleoprotein immunoprecipitation (RIP)
ASJC Scopus subject areas
- Molecular Biology