Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells: Native ribonucleoprotein immunoprecipitation (RIP) assays

Joseph A. Cozzitorto, Masaya Jimbo, Saswati Chand, Fernando Blanco, Shruti Lal, Melissa Gilbert, Jordan M. Winter, Myriam Gorospe, Jonathan R. Brody

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Post-transcriptional regulation of mRNA can potently dictate protein expression patterns in eukaryotic cells. This mode of regulation occurs through cis -acting regulatory regions in the mRNA transcript that mediate direct interactions with trans -acting RNA-binding proteins (RBPs). This mRNA/protein interaction can be studied in numerous ways that range from in vitro to in vivo through messenger ribonucleoprotein immunoprecipitation (mRNP-IP or RIP) assays. This modified immunoprecipitation approach is an important and sensitive method to determine the regulation of gene expression by specific RBPs under different cellular stressors.

Original languageEnglish (US)
Pages (from-to)239-246
Number of pages8
JournalMethods in Molecular Biology
Volume1262
DOIs
StatePublished - 2015

Keywords

  • Post-transcriptional gene regulation
  • RIP-seq
  • RNA
  • RNA-binding proteins
  • Ribonucleoprotein immunoprecipitation (RIP)

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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