Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells: Native ribonucleoprotein immunoprecipitation (RIP) assays

Joseph A. Cozzitorto; Masaya Jimbo; Saswati Chand; Fernando Blanco; Shruti Lal; Melissa Gilbert; Jordan M. Winter; Myriam Gorospe; Jonathan R. Brody

doi:10.1007/978-1-4939-2253-6_14

Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells: Native ribonucleoprotein immunoprecipitation (RIP) assays

Joseph A. Cozzitorto, Masaya Jimbo, Saswati Chand, Fernando Blanco, Shruti Lal, Melissa Gilbert, Jordan M. Winter, Myriam Gorospe, Jonathan R. Brody

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Abstract

Post-transcriptional regulation of mRNA can potently dictate protein expression patterns in eukaryotic cells. This mode of regulation occurs through cis -acting regulatory regions in the mRNA transcript that mediate direct interactions with trans -acting RNA-binding proteins (RBPs). This mRNA/protein interaction can be studied in numerous ways that range from in vitro to in vivo through messenger ribonucleoprotein immunoprecipitation (mRNP-IP or RIP) assays. This modified immunoprecipitation approach is an important and sensitive method to determine the regulation of gene expression by specific RBPs under different cellular stressors.

Original language	English (US)
Pages (from-to)	239-246
Number of pages	8
Journal	Methods in Molecular Biology
Volume	1262
DOIs	https://doi.org/10.1007/978-1-4939-2253-6_14
State	Published - 2015

Keywords

Post-transcriptional gene regulation
RIP-seq
RNA
RNA-binding proteins
Ribonucleoprotein immunoprecipitation (RIP)

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-2253-6_14

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title = "Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells: Native ribonucleoprotein immunoprecipitation (RIP) assays",

abstract = "Post-transcriptional regulation of mRNA can potently dictate protein expression patterns in eukaryotic cells. This mode of regulation occurs through cis -acting regulatory regions in the mRNA transcript that mediate direct interactions with trans -acting RNA-binding proteins (RBPs). This mRNA/protein interaction can be studied in numerous ways that range from in vitro to in vivo through messenger ribonucleoprotein immunoprecipitation (mRNP-IP or RIP) assays. This modified immunoprecipitation approach is an important and sensitive method to determine the regulation of gene expression by specific RBPs under different cellular stressors.",

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doi = "10.1007/978-1-4939-2253-6_14",

language = "English (US)",

volume = "1262",

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T1 - Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells

T2 - Native ribonucleoprotein immunoprecipitation (RIP) assays

AU - Cozzitorto, Joseph A.

AU - Jimbo, Masaya

AU - Chand, Saswati

AU - Blanco, Fernando

AU - Lal, Shruti

AU - Gilbert, Melissa

AU - Winter, Jordan M.

AU - Gorospe, Myriam

AU - Brody, Jonathan R.

N1 - Publisher Copyright: © Springer Science+Business Media New York 2015.

PY - 2015

Y1 - 2015

N2 - Post-transcriptional regulation of mRNA can potently dictate protein expression patterns in eukaryotic cells. This mode of regulation occurs through cis -acting regulatory regions in the mRNA transcript that mediate direct interactions with trans -acting RNA-binding proteins (RBPs). This mRNA/protein interaction can be studied in numerous ways that range from in vitro to in vivo through messenger ribonucleoprotein immunoprecipitation (mRNP-IP or RIP) assays. This modified immunoprecipitation approach is an important and sensitive method to determine the regulation of gene expression by specific RBPs under different cellular stressors.

AB - Post-transcriptional regulation of mRNA can potently dictate protein expression patterns in eukaryotic cells. This mode of regulation occurs through cis -acting regulatory regions in the mRNA transcript that mediate direct interactions with trans -acting RNA-binding proteins (RBPs). This mRNA/protein interaction can be studied in numerous ways that range from in vitro to in vivo through messenger ribonucleoprotein immunoprecipitation (mRNP-IP or RIP) assays. This modified immunoprecipitation approach is an important and sensitive method to determine the regulation of gene expression by specific RBPs under different cellular stressors.

KW - Post-transcriptional gene regulation

KW - RIP-seq

KW - RNA

KW - RNA-binding proteins

KW - Ribonucleoprotein immunoprecipitation (RIP)

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JO - Methods in Molecular Biology

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Studying RNA-binding protein interactions with target mRNAs in eukaryotic cells: Native ribonucleoprotein immunoprecipitation (RIP) assays

Abstract

Keywords

ASJC Scopus subject areas

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