Structure and function of the yeast tRNA ligase gene

Shawn Westaway, E. M. Phizicky, J. Abelson

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We report here the DNA sequence of the entire coding region of the Saccharomyces cerevisiae tRNA ligase gene. tRNA ligase is one of two enzymes required for tRNA splicing in yeast, and the enzyme is likely a single polypeptide with multiple activities. We find that tRNA ligase is a basic protein of 827 amino acids corresponding to a molecular weight of approximately 95,400. The inferred amino acid sequence for tRNA ligase is not significantly homologous to that of other known proteins of similar activity. In addition to the tRNA ligase reading frame and several other unidentified open reading frames, we have found two open reading frames, ORF1 and ORF2, near the 5'-end of the ligase structural gene. One of these, ORF2, produces a divergent transcript which initiates only 125 nucleotides upstream of the tRNA ligase transcript, and is present in approximately the same relative abundance as the transcript for tRNA ligase.

Original languageEnglish (US)
Pages (from-to)3171-3176
Number of pages6
JournalJournal of Biological Chemistry
Volume263
Issue number7
StatePublished - 1988
Externally publishedYes

Fingerprint

RNA Ligase (ATP)
Genes
Open Reading Frames
Yeast
Reading Frames
Amino Acids
Enzymes
Ligases
Transfer RNA
DNA sequences
Saccharomyces cerevisiae
yeast tRNA ligase
Amino Acid Sequence
Proteins
Nucleotides
Yeasts
Molecular Weight
Molecular weight
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Westaway, S., Phizicky, E. M., & Abelson, J. (1988). Structure and function of the yeast tRNA ligase gene. Journal of Biological Chemistry, 263(7), 3171-3176.

Structure and function of the yeast tRNA ligase gene. / Westaway, Shawn; Phizicky, E. M.; Abelson, J.

In: Journal of Biological Chemistry, Vol. 263, No. 7, 1988, p. 3171-3176.

Research output: Contribution to journalArticle

Westaway, S, Phizicky, EM & Abelson, J 1988, 'Structure and function of the yeast tRNA ligase gene', Journal of Biological Chemistry, vol. 263, no. 7, pp. 3171-3176.
Westaway, Shawn ; Phizicky, E. M. ; Abelson, J. / Structure and function of the yeast tRNA ligase gene. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 7. pp. 3171-3176.
@article{f032314e6f434b01ae706057fa62e0ea,
title = "Structure and function of the yeast tRNA ligase gene",
abstract = "We report here the DNA sequence of the entire coding region of the Saccharomyces cerevisiae tRNA ligase gene. tRNA ligase is one of two enzymes required for tRNA splicing in yeast, and the enzyme is likely a single polypeptide with multiple activities. We find that tRNA ligase is a basic protein of 827 amino acids corresponding to a molecular weight of approximately 95,400. The inferred amino acid sequence for tRNA ligase is not significantly homologous to that of other known proteins of similar activity. In addition to the tRNA ligase reading frame and several other unidentified open reading frames, we have found two open reading frames, ORF1 and ORF2, near the 5'-end of the ligase structural gene. One of these, ORF2, produces a divergent transcript which initiates only 125 nucleotides upstream of the tRNA ligase transcript, and is present in approximately the same relative abundance as the transcript for tRNA ligase.",
author = "Shawn Westaway and Phizicky, {E. M.} and J. Abelson",
year = "1988",
language = "English (US)",
volume = "263",
pages = "3171--3176",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "7",

}

TY - JOUR

T1 - Structure and function of the yeast tRNA ligase gene

AU - Westaway, Shawn

AU - Phizicky, E. M.

AU - Abelson, J.

PY - 1988

Y1 - 1988

N2 - We report here the DNA sequence of the entire coding region of the Saccharomyces cerevisiae tRNA ligase gene. tRNA ligase is one of two enzymes required for tRNA splicing in yeast, and the enzyme is likely a single polypeptide with multiple activities. We find that tRNA ligase is a basic protein of 827 amino acids corresponding to a molecular weight of approximately 95,400. The inferred amino acid sequence for tRNA ligase is not significantly homologous to that of other known proteins of similar activity. In addition to the tRNA ligase reading frame and several other unidentified open reading frames, we have found two open reading frames, ORF1 and ORF2, near the 5'-end of the ligase structural gene. One of these, ORF2, produces a divergent transcript which initiates only 125 nucleotides upstream of the tRNA ligase transcript, and is present in approximately the same relative abundance as the transcript for tRNA ligase.

AB - We report here the DNA sequence of the entire coding region of the Saccharomyces cerevisiae tRNA ligase gene. tRNA ligase is one of two enzymes required for tRNA splicing in yeast, and the enzyme is likely a single polypeptide with multiple activities. We find that tRNA ligase is a basic protein of 827 amino acids corresponding to a molecular weight of approximately 95,400. The inferred amino acid sequence for tRNA ligase is not significantly homologous to that of other known proteins of similar activity. In addition to the tRNA ligase reading frame and several other unidentified open reading frames, we have found two open reading frames, ORF1 and ORF2, near the 5'-end of the ligase structural gene. One of these, ORF2, produces a divergent transcript which initiates only 125 nucleotides upstream of the tRNA ligase transcript, and is present in approximately the same relative abundance as the transcript for tRNA ligase.

UR - http://www.scopus.com/inward/record.url?scp=0023834872&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023834872&partnerID=8YFLogxK

M3 - Article

C2 - 3277966

AN - SCOPUS:0023834872

VL - 263

SP - 3171

EP - 3176

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 7

ER -