Structural studies of human basement-membrane collagen with the use of a monoclonal antibody

H. Dieringer, D. W. Hollister, R. W. Glanville, Lynn Sakai, K. Kühn

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A monoclonal antibody monospecific for human type IV collagen was used as a structural probe to examine aspects of the macromolecular organization of basement-membrane collagen. Electron-microscopic observation of rotary-shadowed antigen-antibody complexes demonstrated a unique binding site for the antibody 55±6 nm distant from the 7S cross-linking region of tetrameric type IV collagen. This observation allowed a series of studies that showed: (1) the localization of an intramolecular disulphide bridge within the helical domain of the molecule, (2) the alignment of major peptic-digest fragment of the α1(IV) chain, and (3) confirmation of the postulated antiparallel arrangement of individual molecules within type IV collagen tetramers.

Original languageEnglish (US)
Pages (from-to)217-222
Number of pages6
JournalBiochemical Journal
Volume227
Issue number1
DOIs
StatePublished - Jan 1 1985
Externally publishedYes

Fingerprint

Collagen Type IV
Basement Membrane
Collagen
Monoclonal Antibodies
Antibody Binding Sites
Molecules
Antigen-Antibody Complex
Disulfides
Digestion
Electrons

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Structural studies of human basement-membrane collagen with the use of a monoclonal antibody. / Dieringer, H.; Hollister, D. W.; Glanville, R. W.; Sakai, Lynn; Kühn, K.

In: Biochemical Journal, Vol. 227, No. 1, 01.01.1985, p. 217-222.

Research output: Contribution to journalArticle

Dieringer, H. ; Hollister, D. W. ; Glanville, R. W. ; Sakai, Lynn ; Kühn, K. / Structural studies of human basement-membrane collagen with the use of a monoclonal antibody. In: Biochemical Journal. 1985 ; Vol. 227, No. 1. pp. 217-222.
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