Structural studies of human basement-membrane collagen with the use of a monoclonal antibody

H. Dieringer, D. W. Hollister, R. W. Glanville, L. Y. Sakai, K. Kühn

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A monoclonal antibody monospecific for human type IV collagen was used as a structural probe to examine aspects of the macromolecular organization of basement-membrane collagen. Electron-microscopic observation of rotary-shadowed antigen-antibody complexes demonstrated a unique binding site for the antibody 55±6 nm distant from the 7S cross-linking region of tetrameric type IV collagen. This observation allowed a series of studies that showed: (1) the localization of an intramolecular disulphide bridge within the helical domain of the molecule, (2) the alignment of major peptic-digest fragment of the α1(IV) chain, and (3) confirmation of the postulated antiparallel arrangement of individual molecules within type IV collagen tetramers.

Original languageEnglish (US)
Pages (from-to)217-222
Number of pages6
JournalBiochemical Journal
Volume227
Issue number1
DOIs
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Structural studies of human basement-membrane collagen with the use of a monoclonal antibody'. Together they form a unique fingerprint.

  • Cite this