Structural characterization of the copper site in galactose oxidase using X-ray absorption spectroscopy

K. Clark, J. E. Penner-Hahn, M. Whittaker, James Whittaker

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

X-ray absorption spectroscopy has been used to characterize the local structural environment of the Cu ion in the reductively inactivated, oxidatively activated, and active+substrate oxidation state derivatives of galactose oxidase. In all three cases, the local environment of the Cu is best modeled by a single shell of low-Z (N or O) scatterers. This is generally consistent with the structure determined crystallographically, although the EXAFS bond lengths are slightly, but significantly, shorter than those found crystallographically. The best-fit average bond lengths are 1.97, 1.95, and 1.98 Å for inactive, active, and active+substrate, respectively. The Cu(II) ion in the active and inactive derivatives has an apparent coordination number of 4, consistent with the equatorial ligation seen crystallographically. The Cu(I) ion in the reduced+substrate derivative appears to have either a lower coordination number or a significantly more distorted local environment. The observed Cu(I)-(N/O) bond length favors a model where the Cu becomes 3-coordinate in the substrate-reduced complex.

Original languageEnglish (US)
Pages (from-to)12553-12557
Number of pages5
JournalBiochemistry
Volume33
Issue number42
DOIs
StatePublished - 1994
Externally publishedYes

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X-Ray Absorption Spectroscopy
Galactose Oxidase
X ray absorption spectroscopy
Copper
Bond length
Ions
Substrates
Derivatives
Ligation
Oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)

Cite this

Structural characterization of the copper site in galactose oxidase using X-ray absorption spectroscopy. / Clark, K.; Penner-Hahn, J. E.; Whittaker, M.; Whittaker, James.

In: Biochemistry, Vol. 33, No. 42, 1994, p. 12553-12557.

Research output: Contribution to journalArticle

Clark, K, Penner-Hahn, JE, Whittaker, M & Whittaker, J 1994, 'Structural characterization of the copper site in galactose oxidase using X-ray absorption spectroscopy', Biochemistry, vol. 33, no. 42, pp. 12553-12557. https://doi.org/10.1021/bi00208a004
Clark, K. ; Penner-Hahn, J. E. ; Whittaker, M. ; Whittaker, James. / Structural characterization of the copper site in galactose oxidase using X-ray absorption spectroscopy. In: Biochemistry. 1994 ; Vol. 33, No. 42. pp. 12553-12557.
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