Structural and functional analysis of the 5'-transcription control region for the human 1/4 -HS glycoprotein gene

F. Banine; C. Gangneux; J. P. Lebreton; T. Frebourg; J. P. Salier

doi:10.1016/S0167-4781(98)00042-6

Structural and functional analysis of the 5'-transcription control region for the human 1/4 -HS glycoprotein gene

F. Banine, C. Gangneux, J. P. Lebreton, T. Frebourg, J. P. Salier

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The human alpha2-HS glycoprotein (A2HS) and rat phosphoprotein of Mr 63000 (pp63) are homologous plasma proteins and members of the fetuin superfamily. A2HS is involved in important functions such as inhbition of the tyrosine kinase activity of the insulin receptor, regulation of calcium metabolism and osteogenesis as well as protease inhibitory activity. We report an analysis of the 5' transcription control region (4 kb) of the A2HS gene. Its most proximal 300 nt display a very potent transcription activity. The latter is likely accounted for by C/EBP and NF1 binding sites that are conserved from the human A2HS gene to the rat pp63 gene. In contrast, these human and rat genes appear to largely diverge beyond their proximal promoter.

Original language	English (US)
Pages (from-to)	1-8
Number of pages	8
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1398
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4781(98)00042-6
State	Published - May 29 1998

Keywords

Hepatocyte
Liver
Liver-enriched nuclear factor
Plasma protein
Transcription

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(98)00042-6

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title = "Structural and functional analysis of the 5'-transcription control region for the human 1/4 -HS glycoprotein gene",

abstract = "The human alpha2-HS glycoprotein (A2HS) and rat phosphoprotein of Mr 63000 (pp63) are homologous plasma proteins and members of the fetuin superfamily. A2HS is involved in important functions such as inhbition of the tyrosine kinase activity of the insulin receptor, regulation of calcium metabolism and osteogenesis as well as protease inhibitory activity. We report an analysis of the 5' transcription control region (4 kb) of the A2HS gene. Its most proximal 300 nt display a very potent transcription activity. The latter is likely accounted for by C/EBP and NF1 binding sites that are conserved from the human A2HS gene to the rat pp63 gene. In contrast, these human and rat genes appear to largely diverge beyond their proximal promoter.",

keywords = "Hepatocyte, Liver, Liver-enriched nuclear factor, Plasma protein, Transcription",

author = "F. Banine and C. Gangneux and Lebreton, {J. P.} and T. Frebourg and Salier, {J. P.}",

note = "Funding Information: We are indebted to Prof. P. Arnaud (Charleston, SC, USA) for the kind gift of a human A2HS cDNA and to Dr. C. N'Guyen (Marseille, France) for the gift of a human genomic library. F. Charbonnier is acknowledged for help with DNA sequencing. F.B. is the recipient of a fellowship from the Ligue Nationale contre le Cancer and C.G. is the recipient of a fellowship from the French Ministry for Research. This work was supported in part by a grant from Groupement de Recherches et d'Etudes sur les G{\'e}nomes to J.P.S. and by the University of Rouen.",

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AU - Gangneux, C.

AU - Lebreton, J. P.

AU - Frebourg, T.

AU - Salier, J. P.

N1 - Funding Information: We are indebted to Prof. P. Arnaud (Charleston, SC, USA) for the kind gift of a human A2HS cDNA and to Dr. C. N'Guyen (Marseille, France) for the gift of a human genomic library. F. Charbonnier is acknowledged for help with DNA sequencing. F.B. is the recipient of a fellowship from the Ligue Nationale contre le Cancer and C.G. is the recipient of a fellowship from the French Ministry for Research. This work was supported in part by a grant from Groupement de Recherches et d'Etudes sur les Génomes to J.P.S. and by the University of Rouen.

PY - 1998/5/29

Y1 - 1998/5/29

N2 - The human alpha2-HS glycoprotein (A2HS) and rat phosphoprotein of Mr 63000 (pp63) are homologous plasma proteins and members of the fetuin superfamily. A2HS is involved in important functions such as inhbition of the tyrosine kinase activity of the insulin receptor, regulation of calcium metabolism and osteogenesis as well as protease inhibitory activity. We report an analysis of the 5' transcription control region (4 kb) of the A2HS gene. Its most proximal 300 nt display a very potent transcription activity. The latter is likely accounted for by C/EBP and NF1 binding sites that are conserved from the human A2HS gene to the rat pp63 gene. In contrast, these human and rat genes appear to largely diverge beyond their proximal promoter.

AB - The human alpha2-HS glycoprotein (A2HS) and rat phosphoprotein of Mr 63000 (pp63) are homologous plasma proteins and members of the fetuin superfamily. A2HS is involved in important functions such as inhbition of the tyrosine kinase activity of the insulin receptor, regulation of calcium metabolism and osteogenesis as well as protease inhibitory activity. We report an analysis of the 5' transcription control region (4 kb) of the A2HS gene. Its most proximal 300 nt display a very potent transcription activity. The latter is likely accounted for by C/EBP and NF1 binding sites that are conserved from the human A2HS gene to the rat pp63 gene. In contrast, these human and rat genes appear to largely diverge beyond their proximal promoter.

KW - Hepatocyte

KW - Liver

KW - Liver-enriched nuclear factor

KW - Plasma protein

KW - Transcription

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Structural and functional analysis of the 5'-transcription control region for the human 1/4 -HS glycoprotein gene

Abstract

Keywords

ASJC Scopus subject areas

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