Steroid production by early pregnancy human placental villi in culture

I. A. Maslar, David Hess, J. G. Buckmaster, J. J. Lazur, F. Z. Stanczyk, M. J. Novy

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Organ cultures prepared from human placentae obtained at 7-12 weeks of gestation were maintained for 3-13 days in Dulbecco's modified Eagle medium (DMEM). The addition of pregnenolone to the medium resulted in a dose-related increase in progesterone production and the addition of androstenedione resulted in a dose related increase in oestrogen production. More oestrone than oestradiol was measured in medium collected at the end of the first day of culture, but, on all subsequent days, oestradiol was the predominant oestrogen produced when androstenedione was added to the culture medium. When villi were incubated with [3H]androstenedione immediately after dissection most of the radiolabelled oestrogen recovered from the tissue and medium was oestrone; however, more [3H]oestradiol was recovered when villi were tested after 3 days of culture in DMEM. The addition of oestrone to the culture medium resulted in a dose related increase in oestradiol production with oestradiol accounting for a larger proportion of the total oestrogen in the day 2 and 3 medium samples than in the day 1 samples. These data demonstrate that the enzymes required for biosynthesis of progesterone and oestrogen from exogenous substrate are maintained for at least 13 days when early pregnancy placental villi are cultured in serum-free DMEM. However, a temporal change in the pattern of oestrogen synthesis does occur in culture, such that oestradiol rather than oestrone becomes the major product of androstenedione metabolism.

    Original languageEnglish (US)
    Pages (from-to)277-288
    Number of pages12
    JournalPlacenta
    Volume11
    Issue number3
    DOIs
    StatePublished - 1990

    Fingerprint

    Chorionic Villi
    Estradiol
    Estrogens
    Androstenedione
    Estrone
    Steroids
    Pregnancy
    Eagles
    Progesterone
    Culture Media
    Pregnenolone
    Organ Culture Techniques
    Placenta
    Dissection
    Enzymes
    Serum

    ASJC Scopus subject areas

    • Obstetrics and Gynecology

    Cite this

    Maslar, I. A., Hess, D., Buckmaster, J. G., Lazur, J. J., Stanczyk, F. Z., & Novy, M. J. (1990). Steroid production by early pregnancy human placental villi in culture. Placenta, 11(3), 277-288. https://doi.org/10.1016/S0143-4004(05)80274-7

    Steroid production by early pregnancy human placental villi in culture. / Maslar, I. A.; Hess, David; Buckmaster, J. G.; Lazur, J. J.; Stanczyk, F. Z.; Novy, M. J.

    In: Placenta, Vol. 11, No. 3, 1990, p. 277-288.

    Research output: Contribution to journalArticle

    Maslar, IA, Hess, D, Buckmaster, JG, Lazur, JJ, Stanczyk, FZ & Novy, MJ 1990, 'Steroid production by early pregnancy human placental villi in culture', Placenta, vol. 11, no. 3, pp. 277-288. https://doi.org/10.1016/S0143-4004(05)80274-7
    Maslar IA, Hess D, Buckmaster JG, Lazur JJ, Stanczyk FZ, Novy MJ. Steroid production by early pregnancy human placental villi in culture. Placenta. 1990;11(3):277-288. https://doi.org/10.1016/S0143-4004(05)80274-7
    Maslar, I. A. ; Hess, David ; Buckmaster, J. G. ; Lazur, J. J. ; Stanczyk, F. Z. ; Novy, M. J. / Steroid production by early pregnancy human placental villi in culture. In: Placenta. 1990 ; Vol. 11, No. 3. pp. 277-288.
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