TY - JOUR
T1 - Staphylococcus aureus nasal colonization among pediatric cystic fibrosis patients and their household contacts
AU - Stone, Anne
AU - Quittell, Lynne
AU - Zhou, Juyan
AU - Alba, Luis
AU - Bhat, Meera
AU - Decelie-Germana, Joan
AU - Rajan, Sujatha
AU - Bonitz, Lynn
AU - Welter, John J.
AU - Dozor, Allen J.
AU - Gherson, Ingrid
AU - Lowy, Franklin D.
AU - Saiman, Lisa
PY - 2009/10
Y1 - 2009/10
N2 - Background: Little is known about the prevalence of Staphylococcus aureus nasal colonization and the epidemiology of methicillin-susceptible and methicillin-resistant S. aureus (MRSA) among cystic fibrosis (CF) patients and their household members. Objectives: We sought to determine the epidemiology of S. aureus among children and adolescents with CF and their household members. Methods: Three CF centers enrolled case subjects with at least 1 MRSA-positive respiratory tract culture from 2001 to 2006 and control subjects with MRSA-negative cultures. S. aureus isolates from the anterior nares of CF subjects and their household members were assessed for staphylococcal chromosomal cassette (SCC) mec type. Strain similarity was determined by pulsed-field gel electrophoresis. Results: S. aureus nasal colonization occurred in 52.4% (22/42), 27.0% (17/63), and 25.0% (72/288) of case, control, and household participants, respectively. Case subjects and their contacts were more likely to harbor MRSA in their nares and be from a multipatient CF family. Of 31 MRSA strains, 10 (32.3%) were SCCmec type IVa, associated with community-acquisition. Overall, 27.6% of 98 households had ≥2 members colonized with closely related isolates. Household members were equally likely to be colonized with closely related strains of MRSA (20/31, 65%) versus MSSA (38/80, 48%). Conclusions: This study demonstrated that household members of CF children harbor both MSSA and MRSA, including CA-MRSA, and that S. aureus is transmitted within CF households. Carriage of S. aureus by household members of CF children may have implications for infection control and treatment strategies. Future studies should monitor the distribution and virulence of SCCmecA types in patients with CF.
AB - Background: Little is known about the prevalence of Staphylococcus aureus nasal colonization and the epidemiology of methicillin-susceptible and methicillin-resistant S. aureus (MRSA) among cystic fibrosis (CF) patients and their household members. Objectives: We sought to determine the epidemiology of S. aureus among children and adolescents with CF and their household members. Methods: Three CF centers enrolled case subjects with at least 1 MRSA-positive respiratory tract culture from 2001 to 2006 and control subjects with MRSA-negative cultures. S. aureus isolates from the anterior nares of CF subjects and their household members were assessed for staphylococcal chromosomal cassette (SCC) mec type. Strain similarity was determined by pulsed-field gel electrophoresis. Results: S. aureus nasal colonization occurred in 52.4% (22/42), 27.0% (17/63), and 25.0% (72/288) of case, control, and household participants, respectively. Case subjects and their contacts were more likely to harbor MRSA in their nares and be from a multipatient CF family. Of 31 MRSA strains, 10 (32.3%) were SCCmec type IVa, associated with community-acquisition. Overall, 27.6% of 98 households had ≥2 members colonized with closely related isolates. Household members were equally likely to be colonized with closely related strains of MRSA (20/31, 65%) versus MSSA (38/80, 48%). Conclusions: This study demonstrated that household members of CF children harbor both MSSA and MRSA, including CA-MRSA, and that S. aureus is transmitted within CF households. Carriage of S. aureus by household members of CF children may have implications for infection control and treatment strategies. Future studies should monitor the distribution and virulence of SCCmecA types in patients with CF.
KW - Adolescent
KW - Infection
KW - Molecular
KW - Respiratory tract
KW - Transmission
UR - http://www.scopus.com/inward/record.url?scp=70349706252&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70349706252&partnerID=8YFLogxK
U2 - 10.1097/INF.0b013e3181a3ad0a
DO - 10.1097/INF.0b013e3181a3ad0a
M3 - Article
C2 - 20135845
AN - SCOPUS:70349706252
SN - 0891-3668
VL - 28
SP - 895
EP - 899
JO - Pediatric Infectious Disease Journal
JF - Pediatric Infectious Disease Journal
IS - 10
ER -