Spatiotemporal dynamics of triglyceride storage in unilocular adipocytes

Michael Chu, Harini Sampath, David Y. Cahana, Christoph A. Kahl, Somwar Romel, Anda Cornea, R. Roberts, Oleg Varlamov

    Research output: Contribution to journalArticlepeer-review

    7 Scopus citations

    Abstract

    The spatiotemporal dynamics of triglyceride (TG) storage in unilocular adipocytes are not well understood. Here we applied ex vivo technology to study trafficking and metabolism of fluorescent fatty acids in adipose tissue explants. Live imaging revealed mul? tiple cytoplasmic nodules surrounding the large central lipid droplet (cLD) of unilocular adi? pocytes. Each cytoplasmic nodule harbors a series of closely associated cellular organelles, including micro-lipid droplets (mLDs), mitochondria, and the endoplasmic reticulum. Exoge? nously added free fatty acids are rapidly adsorbed by mLDs and concurrently get esterified to TG. This process is greatly accelerated by insulin. mLDs transfer their content to the cLD, serving as intermediates that mediate packaging of newly synthesized TG in the large inte? rior of a unilocular adipocyte. This study reveals novel cell biological features that may con? tribute to the mechanism of adipocyte hypertrophy.

    Original languageEnglish (US)
    Pages (from-to)4096-4105
    Number of pages10
    JournalMolecular biology of the cell
    Volume25
    Issue number25
    DOIs
    StatePublished - Dec 15 2014

    ASJC Scopus subject areas

    • Molecular Biology
    • Cell Biology

    Fingerprint

    Dive into the research topics of 'Spatiotemporal dynamics of triglyceride storage in unilocular adipocytes'. Together they form a unique fingerprint.

    Cite this