Spatially resolved monitoring of neutrophil elastase activity with ratiometric fluorescent reporters

Stefanie Gehrig; Marcus A. Mall; Carsten Schultz

doi:10.1002/anie.201109226

Spatially resolved monitoring of neutrophil elastase activity with ratiometric fluorescent reporters

Stefanie Gehrig, Marcus A. Mall, Carsten Schultz

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Finding NEmo: In a mouse model for lung inflammation, the lipidated peptide-based ratiometric fluorescent reporter NEmo-2 indicates that neutrophil elastase (NE) activity is mainly associated with the surface of neutrophils (see scheme), whereas a soluble reporter variant showed no activity in the lung fluid, likely because of the abundance of antiproteases. Targeting the localization of NE activity has great potential for the development of improved antiinflammatory drugs.

Original language	English (US)
Pages (from-to)	6258-6261
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	25
DOIs	https://doi.org/10.1002/anie.201109226
State	Published - Jun 18 2012
Externally published	Yes

Keywords

FRET
fluorescent probes
lung emphysema
medicinal chemistry
proteases

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.201109226

Cite this

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abstract = "Finding NEmo: In a mouse model for lung inflammation, the lipidated peptide-based ratiometric fluorescent reporter NEmo-2 indicates that neutrophil elastase (NE) activity is mainly associated with the surface of neutrophils (see scheme), whereas a soluble reporter variant showed no activity in the lung fluid, likely because of the abundance of antiproteases. Targeting the localization of NE activity has great potential for the development of improved antiinflammatory drugs.",

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AU - Schultz, Carsten

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AB - Finding NEmo: In a mouse model for lung inflammation, the lipidated peptide-based ratiometric fluorescent reporter NEmo-2 indicates that neutrophil elastase (NE) activity is mainly associated with the surface of neutrophils (see scheme), whereas a soluble reporter variant showed no activity in the lung fluid, likely because of the abundance of antiproteases. Targeting the localization of NE activity has great potential for the development of improved antiinflammatory drugs.

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KW - fluorescent probes

KW - lung emphysema

KW - medicinal chemistry

KW - proteases

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Spatially resolved monitoring of neutrophil elastase activity with ratiometric fluorescent reporters

Abstract

Keywords

ASJC Scopus subject areas

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