Spatially resolved monitoring of neutrophil elastase activity with ratiometric fluorescent reporters

Stefanie Gehrig, Marcus A. Mall, Carsten Schultz

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Finding NEmo: In a mouse model for lung inflammation, the lipidated peptide-based ratiometric fluorescent reporter NEmo-2 indicates that neutrophil elastase (NE) activity is mainly associated with the surface of neutrophils (see scheme), whereas a soluble reporter variant showed no activity in the lung fluid, likely because of the abundance of antiproteases. Targeting the localization of NE activity has great potential for the development of improved antiinflammatory drugs.

Original languageEnglish (US)
Pages (from-to)6258-6261
Number of pages4
JournalAngewandte Chemie - International Edition
Volume51
Issue number25
DOIs
StatePublished - Jun 18 2012

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Keywords

  • FRET
  • fluorescent probes
  • lung emphysema
  • medicinal chemistry
  • proteases

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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