Soluble forms of herpes simplex virus glycoprotein D bind to a limited number of cell surface receptors and inhibit virus entry into cells

David Johnson, Rae Lyn Burke, Timothy Gregory

Research output: Contribution to journalArticle

125 Citations (Scopus)

Abstract

Herpes simplex virus type 1 (HSV-1) and HSV-2 plaque production was inhibited by treating cells with soluble forms of HSV-1 glycoprotein D (gD-1t) and HSV-2 glycoprotein D (gD-2t). Both glycoproteins inhibited entry of HSV-1 and HSV-2 without affecting virus adsorption. In contrast, a soluble form of HSV-2 glycoprotein B had no effect on virus entry into cells. Specific binding of gD-1t and gD-2t to cells was saturable, and approximately 4 × 105 to 5 × 105 molecules bound per cell. Binding of gD-1t was markedly reduced by treating cells with certain proteases but was unaffected when cell surface heparan sulfate glycosaminoglycans were enzymatically removed or when the binding was carried out in the presence of heparin. Together, these results suggest that gD binds to a limited set of cell surface receptors which may be proteins and that these interactions are essential for subsequent virus entry into cells. However, binding of gD to its receptors is not required for the initial adsorption of virus to the cell surface, which involves more numerous sites (probably including heparan sulfate) than those which mediate gD binding.

Original languageEnglish (US)
Pages (from-to)2569-2576
Number of pages8
JournalJournal of Virology
Volume64
Issue number6
StatePublished - 1990
Externally publishedYes

Fingerprint

herpes simplex
Virus Internalization
Cell Surface Receptors
Simplexvirus
glycoproteins
Glycoproteins
viruses
receptors
Human herpesvirus 1
Human Herpesvirus 1
cells
Human Herpesvirus 2
Heparitin Sulfate
Adsorption
adsorption
Viruses
Glycosaminoglycans
glycosaminoglycans
heparin
Heparin

ASJC Scopus subject areas

  • Immunology

Cite this

Soluble forms of herpes simplex virus glycoprotein D bind to a limited number of cell surface receptors and inhibit virus entry into cells. / Johnson, David; Burke, Rae Lyn; Gregory, Timothy.

In: Journal of Virology, Vol. 64, No. 6, 1990, p. 2569-2576.

Research output: Contribution to journalArticle

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