Sodium ion movements and the spontaneous and electrically stimulated release of [3H]GABA and [14C]glutamic acid from rat cortical slices

John P. Hammerstad, Robert W.P. Cutler

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

The effect on spontaneous and electrically stimulated release of [3H]GABA and [14C]glutamic acid from rat cortical slices of agents and ionic conditions that affect sodium ion movements, was studied in an in vitro superfusion system. Those conditions that elevated non-inulin Na+ content of the slice accelerated spontaneous efflux and inhibited electrically stimulated release of the labeled amino acids. Agents which blocked the entry of Na+ with electrical field stimulation failed to diminish release of the amino acids. Therefore, the entry of Na+ into the slice with membrane depolarization is not a requirement for amino acid neurotransmitter release from cortical slices in response to electrical field stimulation. The acceleration of spontaneous efflux and inhibition of electrically stimulated release in the presence of an elevated non-inulin Na+ content was discussed in relation to sodium coupled transport of amino acids.

Original languageEnglish (US)
Pages (from-to)401-413
Number of pages13
JournalBrain research
Volume47
Issue number2
DOIs
StatePublished - Dec 12 1972

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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