Slow Degeneration of Zebrafish Rohon-Beard Neurons during Programmed Cell Death

Rosario Reyes, Melissa Haendel, Deanna Grant, Ellie Melancon, Judith S. Eisen

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Rohon-Beard cells are large, mechanosensory neurons located in the dorsal spinal cord of anamniote vertebrates. In most species studied to date, these cells die during development. We followed labeled Rohon-Beard cells in living zebrafish embryos and found that they degenerate slowly, over many days. During degeneration, the soma shrinks and finally disappears, and the processes become beady in appearance and finally break apart, but they do not retract. Zebrafish Rohon-Beard cells apparently fragment their DNA, as revealed by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) labeling, before undergoing degenerative morphologic changes. We also followed the development of labeled dorsal root ganglion neurons, as they are developing at the same stages that Rohon-Beard cells are degenerating. We found that, although axons of both cell types extend into similar regions, Rohon-Beard cells degenerate normally in mutants lacking dorsal root ganglia, providing evidence that interactions between the two cell types are not responsible for Rohon-Beard cell degeneration.

Original languageEnglish (US)
Pages (from-to)30-41
Number of pages12
JournalDevelopmental Dynamics
Volume229
Issue number1
DOIs
StatePublished - Jan 2004
Externally publishedYes

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Zebrafish
Cell Death
Neurons
Spinal Ganglia
DNA Nucleotidylexotransferase
Carisoprodol
Axons
Vertebrates
Spinal Cord
Embryonic Structures
DNA

Keywords

  • Apoptosis
  • Autophagy
  • DRG neuron
  • TUNEL

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Slow Degeneration of Zebrafish Rohon-Beard Neurons during Programmed Cell Death. / Reyes, Rosario; Haendel, Melissa; Grant, Deanna; Melancon, Ellie; Eisen, Judith S.

In: Developmental Dynamics, Vol. 229, No. 1, 01.2004, p. 30-41.

Research output: Contribution to journalArticle

Reyes, Rosario ; Haendel, Melissa ; Grant, Deanna ; Melancon, Ellie ; Eisen, Judith S. / Slow Degeneration of Zebrafish Rohon-Beard Neurons during Programmed Cell Death. In: Developmental Dynamics. 2004 ; Vol. 229, No. 1. pp. 30-41.
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