Slit-scan cytometry of mammalian chromosomes

Joe Gray, D. Peters, J. T. Merrill

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A flow cytometer has been constructed which measures total fluorescence and the distribution of fluorescence along isolated, stained mammalian chromosomes. In this device, chromosomes flow lengthwise at 4 m/sec through a 1-μm thick laser beam. The fluorescence from each chromosome is recorded at 10 nsec intervals; the sequence of recorded values represents the distribution of fluorescence along the chromosome and is stored in the memory of a waveform recorder. The total fluorescence of each chromosome is also measured and recorded. Preliminary studies show that doublets of 1.83 μm diameter microspheres flow with their long axes parallel to the direction of flow and that the two microspheres are resolved in the slit-scan profile. Ethidium bromide stained Muntjac and Chinese hamster chromosomes have also been slit-scanned. Centromeres were resolved in many of the Nos. 1 and 2 Chinese hamster chromosomes and the Nos. 1 and X + 3 Muntjac chromosomes.

Original languageEnglish (US)
Pages (from-to)441-444
Number of pages4
JournalJournal of Histochemistry and Cytochemistry
Volume27
Issue number1
StatePublished - 1979
Externally publishedYes

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Mammalian Chromosomes
Chromosomes
Fluorescence
Muntjacs
Cricetulus
Microspheres
Chromosomes, Human, Pair 3
Ethidium
Centromere
Chromosomes, Human, Pair 1
Lasers
Equipment and Supplies

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Slit-scan cytometry of mammalian chromosomes. / Gray, Joe; Peters, D.; Merrill, J. T.

In: Journal of Histochemistry and Cytochemistry, Vol. 27, No. 1, 1979, p. 441-444.

Research output: Contribution to journalArticle

Gray, J, Peters, D & Merrill, JT 1979, 'Slit-scan cytometry of mammalian chromosomes', Journal of Histochemistry and Cytochemistry, vol. 27, no. 1, pp. 441-444.
Gray, Joe ; Peters, D. ; Merrill, J. T. / Slit-scan cytometry of mammalian chromosomes. In: Journal of Histochemistry and Cytochemistry. 1979 ; Vol. 27, No. 1. pp. 441-444.
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