Single microtubules from squid axoplasm support bidirectional movement of organelles

Bruce Schnapp, Ronald D. Vale, Michael P. Sheetz, Thomas S. Reese

Research output: Contribution to journalArticle

218 Citations (Scopus)

Abstract

Single filaments, dissociated from the extruded axoplasm of the squid giant axon and visualized by videoenhanced differential interference contrast microscopy, transport organelles bidirectionally. Organelles moving in the same or opposite directions along the same filament can pass each other without colliding, indicating that each transport filament has several tracks for organelle movement. In order to characterize transport filaments, organelle movements were first examined by video microscopy, and then the same filaments were examined by electron microscopy after rapid-freezing, freeze-drying, and rotaryshadowing. Transport filaments that supported bidirectional movement of organelles are 22 nm to 27 nm in diameter and have a substructure indicative of a single microtubule. Immunofluorescence showed that virtually all transport filaments contain tubulin. These results show that single microtubules can serve as a substratum for organelle movement, and suggest that an interaction between organelles and microtubules is the basis of fast axonal transport.

Original languageEnglish (US)
Pages (from-to)455-462
Number of pages8
JournalCell
Volume40
Issue number2
DOIs
StatePublished - 1985
Externally publishedYes

Fingerprint

Decapodiformes
Microtubules
Organelles
Microscopic examination
Tubulin
Freezing
Electron microscopy
Drying
Interference Microscopy
Video Microscopy
Axonal Transport
Freeze Drying
Fluorescent Antibody Technique
Axons
Electron Microscopy
Direction compound

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Single microtubules from squid axoplasm support bidirectional movement of organelles. / Schnapp, Bruce; Vale, Ronald D.; Sheetz, Michael P.; Reese, Thomas S.

In: Cell, Vol. 40, No. 2, 1985, p. 455-462.

Research output: Contribution to journalArticle

Schnapp, Bruce ; Vale, Ronald D. ; Sheetz, Michael P. ; Reese, Thomas S. / Single microtubules from squid axoplasm support bidirectional movement of organelles. In: Cell. 1985 ; Vol. 40, No. 2. pp. 455-462.
@article{8c5fa9e1f90f4f928ff54aa02049bdde,
title = "Single microtubules from squid axoplasm support bidirectional movement of organelles",
abstract = "Single filaments, dissociated from the extruded axoplasm of the squid giant axon and visualized by videoenhanced differential interference contrast microscopy, transport organelles bidirectionally. Organelles moving in the same or opposite directions along the same filament can pass each other without colliding, indicating that each transport filament has several tracks for organelle movement. In order to characterize transport filaments, organelle movements were first examined by video microscopy, and then the same filaments were examined by electron microscopy after rapid-freezing, freeze-drying, and rotaryshadowing. Transport filaments that supported bidirectional movement of organelles are 22 nm to 27 nm in diameter and have a substructure indicative of a single microtubule. Immunofluorescence showed that virtually all transport filaments contain tubulin. These results show that single microtubules can serve as a substratum for organelle movement, and suggest that an interaction between organelles and microtubules is the basis of fast axonal transport.",
author = "Bruce Schnapp and Vale, {Ronald D.} and Sheetz, {Michael P.} and Reese, {Thomas S.}",
year = "1985",
doi = "10.1016/0092-8674(85)90160-6",
language = "English (US)",
volume = "40",
pages = "455--462",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - Single microtubules from squid axoplasm support bidirectional movement of organelles

AU - Schnapp, Bruce

AU - Vale, Ronald D.

AU - Sheetz, Michael P.

AU - Reese, Thomas S.

PY - 1985

Y1 - 1985

N2 - Single filaments, dissociated from the extruded axoplasm of the squid giant axon and visualized by videoenhanced differential interference contrast microscopy, transport organelles bidirectionally. Organelles moving in the same or opposite directions along the same filament can pass each other without colliding, indicating that each transport filament has several tracks for organelle movement. In order to characterize transport filaments, organelle movements were first examined by video microscopy, and then the same filaments were examined by electron microscopy after rapid-freezing, freeze-drying, and rotaryshadowing. Transport filaments that supported bidirectional movement of organelles are 22 nm to 27 nm in diameter and have a substructure indicative of a single microtubule. Immunofluorescence showed that virtually all transport filaments contain tubulin. These results show that single microtubules can serve as a substratum for organelle movement, and suggest that an interaction between organelles and microtubules is the basis of fast axonal transport.

AB - Single filaments, dissociated from the extruded axoplasm of the squid giant axon and visualized by videoenhanced differential interference contrast microscopy, transport organelles bidirectionally. Organelles moving in the same or opposite directions along the same filament can pass each other without colliding, indicating that each transport filament has several tracks for organelle movement. In order to characterize transport filaments, organelle movements were first examined by video microscopy, and then the same filaments were examined by electron microscopy after rapid-freezing, freeze-drying, and rotaryshadowing. Transport filaments that supported bidirectional movement of organelles are 22 nm to 27 nm in diameter and have a substructure indicative of a single microtubule. Immunofluorescence showed that virtually all transport filaments contain tubulin. These results show that single microtubules can serve as a substratum for organelle movement, and suggest that an interaction between organelles and microtubules is the basis of fast axonal transport.

UR - http://www.scopus.com/inward/record.url?scp=0022019741&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022019741&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(85)90160-6

DO - 10.1016/0092-8674(85)90160-6

M3 - Article

VL - 40

SP - 455

EP - 462

JO - Cell

JF - Cell

SN - 0092-8674

IS - 2

ER -