Single-cell RNA sequencing reveals immunological rewiring at the maternal-fetal interface following asymptomatic/mild SARS-CoV-2 infection

Suhas Sureshchandra, Michael Z. Zulu, Brianna M. Doratt, Allen Jankeel, Delia Tifrea, Robert Edwards, Monica Rincon, Nicole E. Marshall, Ilhem Messaoudi

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

While severe coronavirus 2019 (COVID-19) is associated with immune activation at the maternal-fetal interface, responses to asymptomatic/mild severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection during pregnancy remain unknown. Here, we assess immunological adaptations in blood and term decidua in response to asymptomatic/mild disease in pregnant women. We report attenuated antigen presentation and type I interferon (IFN) signaling pathways, loss of tissue-resident decidual macrophages, and upregulated cytokine/chemokine signaling in monocyte-derived decidual macrophages. Furthermore, we describe increased frequencies of activated tissue-resident T cells and decreased abundance of regulatory T cells with infection while frequencies of cytotoxic CD4/CD8 T cells are increased in the blood. In contrast to decidual macrophages, type I IFN signaling is higher in decidual T cells. Finally, infection leads to a narrowing of T cell receptor diversity in both blood and decidua. Collectively, these observations indicate that asymptomatic/mild COVID-19 during pregnancy results in remodeling of the immunological landscape of the maternal-fetal interface, with a potential for long-term adverse outcomes for the offspring.

Original languageEnglish (US)
Article number110938
JournalCell Reports
Volume39
Issue number11
DOIs
StatePublished - Jun 14 2022

Keywords

  • COVID-19
  • CP: Immunology
  • CP: Microbiology
  • SARS-CoV-2
  • T cells
  • TCR
  • decidua
  • macrophages
  • placenta
  • pregnancy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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