Single cell cloning of epstein-barr virus transformed cells in 20 μl hanging drops

Frances Makowski, Max I. Joffe, Marvin B. Rittenberg

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

A simple method for cloning EBV transformants from single cells in 20 μl hanging drops is described. The hanging-drop method allows direct verification prior to the addition of irradiated feeder cells that clones will be established from single cells. The average efficiency of cloning from wells containing a single transformed cell was 74%, ranging from 60 to 95% in eight separate experiments.

Original languageEnglish (US)
Pages (from-to)85-87
Number of pages3
JournalJournal of Immunological Methods
Volume90
Issue number1
DOIs
StatePublished - Jun 10 1986

Keywords

  • Epstein-Barr virus transformed cells
  • Hanging drop
  • Single cell cloning

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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