Single cell cloning of epstein-barr virus transformed cells in 20 μl hanging drops

Frances Makowski; Max I. Joffe; Marvin B. Rittenberg

doi:10.1016/0022-1759(86)90387-X

Single cell cloning of epstein-barr virus transformed cells in 20 μl hanging drops

Frances Makowski, Max I. Joffe, Marvin B. Rittenberg

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

A simple method for cloning EBV transformants from single cells in 20 μl hanging drops is described. The hanging-drop method allows direct verification prior to the addition of irradiated feeder cells that clones will be established from single cells. The average efficiency of cloning from wells containing a single transformed cell was 74%, ranging from 60 to 95% in eight separate experiments.

Original language	English (US)
Pages (from-to)	85-87
Number of pages	3
Journal	Journal of Immunological Methods
Volume	90
Issue number	1
DOIs	https://doi.org/10.1016/0022-1759(86)90387-X
State	Published - Jun 10 1986
Externally published	Yes

Keywords

Epstein-Barr virus transformed cells
Hanging drop
Single cell cloning

ASJC Scopus subject areas

Immunology and Allergy
Immunology

Access to Document

10.1016/0022-1759(86)90387-X

Cite this

@article{e84248696b7c48c784d44a527d85fd71,

title = "Single cell cloning of epstein-barr virus transformed cells in 20 μl hanging drops",

abstract = "A simple method for cloning EBV transformants from single cells in 20 μl hanging drops is described. The hanging-drop method allows direct verification prior to the addition of irradiated feeder cells that clones will be established from single cells. The average efficiency of cloning from wells containing a single transformed cell was 74%, ranging from 60 to 95% in eight separate experiments.",

keywords = "Epstein-Barr virus transformed cells, Hanging drop, Single cell cloning",

author = "Frances Makowski and Joffe, {Max I.} and Rittenberg, {Marvin B.}",

note = "Funding Information: This work was supported in part by NIH Grant AI 14985 and U.S. Army Contract DAMD 17-83-C-3246.",

year = "1986",

month = jun,

day = "10",

doi = "10.1016/0022-1759(86)90387-X",

language = "English (US)",

volume = "90",

pages = "85--87",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Single cell cloning of epstein-barr virus transformed cells in 20 μl hanging drops

AU - Makowski, Frances

AU - Joffe, Max I.

AU - Rittenberg, Marvin B.

N1 - Funding Information: This work was supported in part by NIH Grant AI 14985 and U.S. Army Contract DAMD 17-83-C-3246.

PY - 1986/6/10

Y1 - 1986/6/10

N2 - A simple method for cloning EBV transformants from single cells in 20 μl hanging drops is described. The hanging-drop method allows direct verification prior to the addition of irradiated feeder cells that clones will be established from single cells. The average efficiency of cloning from wells containing a single transformed cell was 74%, ranging from 60 to 95% in eight separate experiments.

AB - A simple method for cloning EBV transformants from single cells in 20 μl hanging drops is described. The hanging-drop method allows direct verification prior to the addition of irradiated feeder cells that clones will be established from single cells. The average efficiency of cloning from wells containing a single transformed cell was 74%, ranging from 60 to 95% in eight separate experiments.

KW - Epstein-Barr virus transformed cells

KW - Hanging drop

KW - Single cell cloning

UR - http://www.scopus.com/inward/record.url?scp=0022516948&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022516948&partnerID=8YFLogxK

U2 - 10.1016/0022-1759(86)90387-X

DO - 10.1016/0022-1759(86)90387-X

M3 - Article

C2 - 3011914

AN - SCOPUS:0022516948

SN - 0022-1759

VL - 90

SP - 85

EP - 87

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1

ER -

Single cell cloning of epstein-barr virus transformed cells in 20 μl hanging drops

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this