A simple method for cloning EBV transformants from single cells in 20 μl hanging drops is described. The hanging-drop method allows direct verification prior to the addition of irradiated feeder cells that clones will be established from single cells. The average efficiency of cloning from wells containing a single transformed cell was 74%, ranging from 60 to 95% in eight separate experiments.
- Epstein-Barr virus transformed cells
- Hanging drop
- Single cell cloning
ASJC Scopus subject areas
- Immunology and Allergy