Single cell cloning of epstein-barr virus transformed cells in 20 μl hanging drops

Frances Makowski, Max I. Joffe, Marvin Rittenberg

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A simple method for cloning EBV transformants from single cells in 20 μl hanging drops is described. The hanging-drop method allows direct verification prior to the addition of irradiated feeder cells that clones will be established from single cells. The average efficiency of cloning from wells containing a single transformed cell was 74%, ranging from 60 to 95% in eight separate experiments.

Original languageEnglish (US)
Pages (from-to)85-87
Number of pages3
JournalJournal of Immunological Methods
Volume90
Issue number1
DOIs
StatePublished - Jun 10 1986

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Human Herpesvirus 4
Organism Cloning
Feeder Cells
Clone Cells

Keywords

  • Epstein-Barr virus transformed cells
  • Hanging drop
  • Single cell cloning

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

Single cell cloning of epstein-barr virus transformed cells in 20 μl hanging drops. / Makowski, Frances; Joffe, Max I.; Rittenberg, Marvin.

In: Journal of Immunological Methods, Vol. 90, No. 1, 10.06.1986, p. 85-87.

Research output: Contribution to journalArticle

Makowski, Frances ; Joffe, Max I. ; Rittenberg, Marvin. / Single cell cloning of epstein-barr virus transformed cells in 20 μl hanging drops. In: Journal of Immunological Methods. 1986 ; Vol. 90, No. 1. pp. 85-87.
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