Simultaneously monitoring immune response and microbial infections during pregnancy through Plasma cfRNA sequencing

Wenying Pan, Thuy T.M. Ngo, Joan Camunas-Soler, Chun Xiao Song, Mark Kowarsky, Yair J. Blumenfeld, Ronald J. Wong, Gary M. Shaw, David K. Stevenson, Stephen R. Quake

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

BACKGROUND: Plasma cell-free RNA (cfRNA) encompasses a broad spectrum of RNA species that can be derived from both human cells and microbes. Because cfRNA is fragmented and of low concentration, it has been challenging to profile its transcriptome using standard RNA-seq methods. METHODS: We assessed several recently developed RNAseq methods on cfRNA samples. We then analyzed the dynamic changes of both the human transcriptome and the microbiome of plasma during pregnancy from 60 women. RESULTS: cfRNA reflects a well-orchestrated immune modulation during pregnancy: an up-regulation of antiinflammatory genes and an increased abundance of antimicrobial genes. We observed that the plasma microbiome remained relatively stable during pregnancy. The bacteria Ureaplasma shows an increased prevalence and increased abundance at postpartum, which is likely to be associated with postpartum infection. We demonstrated that cfRNA-seq can be used to monitor viral infections. We detected a number of human pathogens in our patients, including an undiagnosed patient with a high load of human parvovirus B19 virus (B19V), which is known to be a potential cause of complications in pregnancy. CONCLUSIONS: Plasma cfRNA-seq demonstrates the potential to simultaneously monitor immune response and microbial infections during pregnancy.

Original languageEnglish (US)
Pages (from-to)1695-1704
Number of pages10
JournalClinical chemistry
Volume63
Issue number11
DOIs
StatePublished - Nov 2017

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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    Pan, W., Ngo, T. T. M., Camunas-Soler, J., Song, C. X., Kowarsky, M., Blumenfeld, Y. J., Wong, R. J., Shaw, G. M., Stevenson, D. K., & Quake, S. R. (2017). Simultaneously monitoring immune response and microbial infections during pregnancy through Plasma cfRNA sequencing. Clinical chemistry, 63(11), 1695-1704. https://doi.org/10.1373/clinchem.2017.273888