Simian varicella virus gene expression during acute and latent infection of rhesus macaques.

Christine Meyer, Amelia Kerns, Alex Barron, Craig Kreklywich, Daniel Streblow, Ilhem Messaoudi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Varicella zoster virus (VZV) is a neurotropic α-herpesvirus that causes chickenpox during primary infection and establishes latency in sensory ganglia. Reactivation of VZV results in herpes zoster and other neurological complications. Our understanding of the VZV transcriptome during acute and latent infection in immune competent individuals remains incomplete. Infection of rhesus macaques with the homologous simian varicella virus (SVV) recapitulates the hallmarks of VZV infection. We therefore characterized the SVV transcriptome by quantitative real-time reverse transcriptase PCR during acute infection in bronchial alveolar lavage (BAL) cells and peripheral blood mononuclear cells, and during latency in sensory ganglia obtained from the same rhesus macaques. During acute infection, all known SVV open reading frames (ORFs) were detected, and the most abundantly expressed ORFs are involved in virus replication and assembly such as the transcriptional activator ORF 63 and the structural proteins ORF 41 and ORF 49. In contrast, latent SVV gene expression is highly restricted. ORF 61, a viral transactivator and latency-associated transcript, is the most prevalent transcript detected in sensory ganglia. We also detected ORFs A, B, 4, 10, 63, 64, 65, 66, and 68 though significantly less frequently than ORF 61. This comprehensive analysis has revealed genes that potentially play a role in the establishment and/or maintenance of SVV latency.

Original languageEnglish (US)
Pages (from-to)600-612
Number of pages13
JournalJournal of NeuroVirology
Volume17
Issue number6
StatePublished - Dec 2011
Externally publishedYes

Fingerprint

Chickenpox
Macaca mulatta
Open Reading Frames
Viruses
Gene Expression
Human Herpesvirus 3
Sensory Ganglia
Infection
Virus Latency
Transcriptome
Alveolar Epithelial Cells
Virus Assembly
Trans-Activators
Herpesviridae
Herpes Zoster
Bronchoalveolar Lavage
Virus Diseases
Virus Replication
Reverse Transcriptase Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction

ASJC Scopus subject areas

  • Virology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Neurology

Cite this

Meyer, C., Kerns, A., Barron, A., Kreklywich, C., Streblow, D., & Messaoudi, I. (2011). Simian varicella virus gene expression during acute and latent infection of rhesus macaques. Journal of NeuroVirology, 17(6), 600-612.

Simian varicella virus gene expression during acute and latent infection of rhesus macaques. / Meyer, Christine; Kerns, Amelia; Barron, Alex; Kreklywich, Craig; Streblow, Daniel; Messaoudi, Ilhem.

In: Journal of NeuroVirology, Vol. 17, No. 6, 12.2011, p. 600-612.

Research output: Contribution to journalArticle

Meyer, C, Kerns, A, Barron, A, Kreklywich, C, Streblow, D & Messaoudi, I 2011, 'Simian varicella virus gene expression during acute and latent infection of rhesus macaques.', Journal of NeuroVirology, vol. 17, no. 6, pp. 600-612.
Meyer, Christine ; Kerns, Amelia ; Barron, Alex ; Kreklywich, Craig ; Streblow, Daniel ; Messaoudi, Ilhem. / Simian varicella virus gene expression during acute and latent infection of rhesus macaques. In: Journal of NeuroVirology. 2011 ; Vol. 17, No. 6. pp. 600-612.
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