Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins

Skyler Jackman, Christopher H. Chen, Selmaan N. Chettih, Shay Q. Neufeld, Iain R. Drew, Chimuanya K. Agba, Isabella Flaquer, Alexis N. Stefano, Thomas J. Kennedy, Justine E. Belinsky, Keiramarie Roberston, Celia C. Beron, Bernardo L. Sabatini, Christopher D. Harvey, Wade G. Regehr

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Optical methods of interrogating neural circuits have emerged as powerful tools for understanding how the brain drives behaviors. Optogenetic proteins are widely used to control neuronal activity, while genetically encoded fluorescent reporters are used to monitor activity. These proteins are often expressed by injecting viruses, which frequently leads to inconsistent experiments due to misalignment of expression and optical components. Here, we describe how silk fibroin films simplify optogenetic experiments by providing targeted delivery of viruses. Films composed of silk fibroin and virus are applied to the surface of implantable optical components. After surgery, silk releases the virus to transduce nearby cells and provide localized expression around optical fibers and endoscopes. Silk films can also be used to express genetically encoded sensors in large cortical regions by using cranial windows coated with a silk/virus mixture. The ease of use and improved performance provided by silk make this a promising approach for optogenetic studies. Jackman et al. show that coating optical implants with silk fibroin mixed with AAV allows single-step implantation and expression of optogenetic proteins like channelrhodopsin and GCaMP.

Original languageEnglish (US)
Pages (from-to)3351-3361
Number of pages11
JournalCell Reports
Volume22
Issue number12
DOIs
StatePublished - Mar 20 2018

Fingerprint

Optogenetics
Fibroins
Silk
Viruses
Proteins
Optical Devices
Virus Release
Optical coatings
Optical Fibers
Endoscopy
Endoscopes
Surgery
Optical fibers
Brain
Experiments
Networks (circuits)
Sensors

Keywords

  • 2-photon calcium imaging
  • biomaterials
  • cranial windows
  • in vivo imaging
  • optical fiber implants
  • optogenetics
  • silk
  • stereotaxic injections
  • tapered optical fibers
  • viral vectors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Jackman, S., Chen, C. H., Chettih, S. N., Neufeld, S. Q., Drew, I. R., Agba, C. K., ... Regehr, W. G. (2018). Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins. Cell Reports, 22(12), 3351-3361. https://doi.org/10.1016/j.celrep.2018.02.081

Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins. / Jackman, Skyler; Chen, Christopher H.; Chettih, Selmaan N.; Neufeld, Shay Q.; Drew, Iain R.; Agba, Chimuanya K.; Flaquer, Isabella; Stefano, Alexis N.; Kennedy, Thomas J.; Belinsky, Justine E.; Roberston, Keiramarie; Beron, Celia C.; Sabatini, Bernardo L.; Harvey, Christopher D.; Regehr, Wade G.

In: Cell Reports, Vol. 22, No. 12, 20.03.2018, p. 3351-3361.

Research output: Contribution to journalArticle

Jackman, S, Chen, CH, Chettih, SN, Neufeld, SQ, Drew, IR, Agba, CK, Flaquer, I, Stefano, AN, Kennedy, TJ, Belinsky, JE, Roberston, K, Beron, CC, Sabatini, BL, Harvey, CD & Regehr, WG 2018, 'Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins', Cell Reports, vol. 22, no. 12, pp. 3351-3361. https://doi.org/10.1016/j.celrep.2018.02.081
Jackman, Skyler ; Chen, Christopher H. ; Chettih, Selmaan N. ; Neufeld, Shay Q. ; Drew, Iain R. ; Agba, Chimuanya K. ; Flaquer, Isabella ; Stefano, Alexis N. ; Kennedy, Thomas J. ; Belinsky, Justine E. ; Roberston, Keiramarie ; Beron, Celia C. ; Sabatini, Bernardo L. ; Harvey, Christopher D. ; Regehr, Wade G. / Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins. In: Cell Reports. 2018 ; Vol. 22, No. 12. pp. 3351-3361.
@article{4951e2c038554739a4ddfa865fe9beb6,
title = "Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins",
abstract = "Optical methods of interrogating neural circuits have emerged as powerful tools for understanding how the brain drives behaviors. Optogenetic proteins are widely used to control neuronal activity, while genetically encoded fluorescent reporters are used to monitor activity. These proteins are often expressed by injecting viruses, which frequently leads to inconsistent experiments due to misalignment of expression and optical components. Here, we describe how silk fibroin films simplify optogenetic experiments by providing targeted delivery of viruses. Films composed of silk fibroin and virus are applied to the surface of implantable optical components. After surgery, silk releases the virus to transduce nearby cells and provide localized expression around optical fibers and endoscopes. Silk films can also be used to express genetically encoded sensors in large cortical regions by using cranial windows coated with a silk/virus mixture. The ease of use and improved performance provided by silk make this a promising approach for optogenetic studies. Jackman et al. show that coating optical implants with silk fibroin mixed with AAV allows single-step implantation and expression of optogenetic proteins like channelrhodopsin and GCaMP.",
keywords = "2-photon calcium imaging, biomaterials, cranial windows, in vivo imaging, optical fiber implants, optogenetics, silk, stereotaxic injections, tapered optical fibers, viral vectors",
author = "Skyler Jackman and Chen, {Christopher H.} and Chettih, {Selmaan N.} and Neufeld, {Shay Q.} and Drew, {Iain R.} and Agba, {Chimuanya K.} and Isabella Flaquer and Stefano, {Alexis N.} and Kennedy, {Thomas J.} and Belinsky, {Justine E.} and Keiramarie Roberston and Beron, {Celia C.} and Sabatini, {Bernardo L.} and Harvey, {Christopher D.} and Regehr, {Wade G.}",
year = "2018",
month = "3",
day = "20",
doi = "10.1016/j.celrep.2018.02.081",
language = "English (US)",
volume = "22",
pages = "3351--3361",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "12",

}

TY - JOUR

T1 - Silk Fibroin Films Facilitate Single-Step Targeted Expression of Optogenetic Proteins

AU - Jackman, Skyler

AU - Chen, Christopher H.

AU - Chettih, Selmaan N.

AU - Neufeld, Shay Q.

AU - Drew, Iain R.

AU - Agba, Chimuanya K.

AU - Flaquer, Isabella

AU - Stefano, Alexis N.

AU - Kennedy, Thomas J.

AU - Belinsky, Justine E.

AU - Roberston, Keiramarie

AU - Beron, Celia C.

AU - Sabatini, Bernardo L.

AU - Harvey, Christopher D.

AU - Regehr, Wade G.

PY - 2018/3/20

Y1 - 2018/3/20

N2 - Optical methods of interrogating neural circuits have emerged as powerful tools for understanding how the brain drives behaviors. Optogenetic proteins are widely used to control neuronal activity, while genetically encoded fluorescent reporters are used to monitor activity. These proteins are often expressed by injecting viruses, which frequently leads to inconsistent experiments due to misalignment of expression and optical components. Here, we describe how silk fibroin films simplify optogenetic experiments by providing targeted delivery of viruses. Films composed of silk fibroin and virus are applied to the surface of implantable optical components. After surgery, silk releases the virus to transduce nearby cells and provide localized expression around optical fibers and endoscopes. Silk films can also be used to express genetically encoded sensors in large cortical regions by using cranial windows coated with a silk/virus mixture. The ease of use and improved performance provided by silk make this a promising approach for optogenetic studies. Jackman et al. show that coating optical implants with silk fibroin mixed with AAV allows single-step implantation and expression of optogenetic proteins like channelrhodopsin and GCaMP.

AB - Optical methods of interrogating neural circuits have emerged as powerful tools for understanding how the brain drives behaviors. Optogenetic proteins are widely used to control neuronal activity, while genetically encoded fluorescent reporters are used to monitor activity. These proteins are often expressed by injecting viruses, which frequently leads to inconsistent experiments due to misalignment of expression and optical components. Here, we describe how silk fibroin films simplify optogenetic experiments by providing targeted delivery of viruses. Films composed of silk fibroin and virus are applied to the surface of implantable optical components. After surgery, silk releases the virus to transduce nearby cells and provide localized expression around optical fibers and endoscopes. Silk films can also be used to express genetically encoded sensors in large cortical regions by using cranial windows coated with a silk/virus mixture. The ease of use and improved performance provided by silk make this a promising approach for optogenetic studies. Jackman et al. show that coating optical implants with silk fibroin mixed with AAV allows single-step implantation and expression of optogenetic proteins like channelrhodopsin and GCaMP.

KW - 2-photon calcium imaging

KW - biomaterials

KW - cranial windows

KW - in vivo imaging

KW - optical fiber implants

KW - optogenetics

KW - silk

KW - stereotaxic injections

KW - tapered optical fibers

KW - viral vectors

UR - http://www.scopus.com/inward/record.url?scp=85044115864&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044115864&partnerID=8YFLogxK

U2 - 10.1016/j.celrep.2018.02.081

DO - 10.1016/j.celrep.2018.02.081

M3 - Article

C2 - 29562189

AN - SCOPUS:85044115864

VL - 22

SP - 3351

EP - 3361

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 12

ER -