Signaling mechanisms of glucagon-like peptide 2-induced intestinal epithelial cell proliferation

Jasleen Jasleen, Naoshi Shimoda, E. Robert Shen, Ali Tavakkolizadeh, Edward E. Whang, Danny Jacobs, Michael J. Zinner, Stanley W. Ashley

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Background. Glucagon-like peptide 2 (GLP-2) stimulates intestinal epithelial growth with high potency and specificity. However, the intracellular signaling pathways responsible for the growth-stimulatory action of GLP-2 are not clearly understood. Here we report possible signaling pathways mediating GLP-2's proliferative actions in the human intestinal epithelial cell line Caco-2. Materials and methods. Caco-2 cells were subcultured under serum-deprived conditions in the presence or absence of GLP-2 (10 μM) and varying concentrations of inhibitors of three candidate kinases: genistein, a global tyrosine kinase inhibitor; LY294002, a phosphatidylinositide (PI) 3-kinase inhibitor; and PD 098059, a mitogen- activated/extracellular signal-regulated kinase (MEK) inhibitor. Proliferation was assessed using [3H]thymidine incorporation. Relative abundance of the phosphorylated forms of two specific mitogen-activated protein kinases (MAPKs), ERK1 and ERK2, was assessed by Western blotting. Results. GLP-2-treated cells demonstrated a greater than 10-fold increase in proliferation. This response was inhibited by genistein, LY294002, and PD 098059 in a dose-dependent fashion. A significantly greater abundance of the phosphorylated forms of both ERK-1 and ERK-2 was present in cells within 5 rain of treatment with GLP-2. Conclusions. GLP-2 stimulates the proliferation of Caco-2 cells in vitro. This increase in Caco-2 proliferation in response to GLP-2 may be due, at least in part, to the involvement of both the PI 3- kinase and the MAPK pathways. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)13-18
Number of pages6
JournalJournal of Surgical Research
Volume90
Issue number1
DOIs
StatePublished - May 1 2000
Externally publishedYes

Fingerprint

Glucagon-Like Peptide 2
Epithelial Cells
Cell Proliferation
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
Caco-2 Cells
Genistein
Phosphotransferases
MAP Kinase Kinase 3
Rain
Mitogen-Activated Protein Kinase Kinases
Extracellular Signal-Regulated MAP Kinases
Growth
Mitogen-Activated Protein Kinases
Mitogens
Protein-Tyrosine Kinases
Thymidine
Western Blotting
Cell Line

Keywords

  • Glucagon-like peptide 2
  • Mitogen-activated protein kinase
  • Mitogen-activated/extracellular signal-regulated kinase
  • Phosphatidylinositide 3-kinase
  • Signal transduction
  • Tyrosine kinase

ASJC Scopus subject areas

  • Surgery

Cite this

Jasleen, J., Shimoda, N., Shen, E. R., Tavakkolizadeh, A., Whang, E. E., Jacobs, D., ... Ashley, S. W. (2000). Signaling mechanisms of glucagon-like peptide 2-induced intestinal epithelial cell proliferation. Journal of Surgical Research, 90(1), 13-18. https://doi.org/10.1006/jsre.2000.5818

Signaling mechanisms of glucagon-like peptide 2-induced intestinal epithelial cell proliferation. / Jasleen, Jasleen; Shimoda, Naoshi; Shen, E. Robert; Tavakkolizadeh, Ali; Whang, Edward E.; Jacobs, Danny; Zinner, Michael J.; Ashley, Stanley W.

In: Journal of Surgical Research, Vol. 90, No. 1, 01.05.2000, p. 13-18.

Research output: Contribution to journalArticle

Jasleen, J, Shimoda, N, Shen, ER, Tavakkolizadeh, A, Whang, EE, Jacobs, D, Zinner, MJ & Ashley, SW 2000, 'Signaling mechanisms of glucagon-like peptide 2-induced intestinal epithelial cell proliferation', Journal of Surgical Research, vol. 90, no. 1, pp. 13-18. https://doi.org/10.1006/jsre.2000.5818
Jasleen, Jasleen ; Shimoda, Naoshi ; Shen, E. Robert ; Tavakkolizadeh, Ali ; Whang, Edward E. ; Jacobs, Danny ; Zinner, Michael J. ; Ashley, Stanley W. / Signaling mechanisms of glucagon-like peptide 2-induced intestinal epithelial cell proliferation. In: Journal of Surgical Research. 2000 ; Vol. 90, No. 1. pp. 13-18.
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abstract = "Background. Glucagon-like peptide 2 (GLP-2) stimulates intestinal epithelial growth with high potency and specificity. However, the intracellular signaling pathways responsible for the growth-stimulatory action of GLP-2 are not clearly understood. Here we report possible signaling pathways mediating GLP-2's proliferative actions in the human intestinal epithelial cell line Caco-2. Materials and methods. Caco-2 cells were subcultured under serum-deprived conditions in the presence or absence of GLP-2 (10 μM) and varying concentrations of inhibitors of three candidate kinases: genistein, a global tyrosine kinase inhibitor; LY294002, a phosphatidylinositide (PI) 3-kinase inhibitor; and PD 098059, a mitogen- activated/extracellular signal-regulated kinase (MEK) inhibitor. Proliferation was assessed using [3H]thymidine incorporation. Relative abundance of the phosphorylated forms of two specific mitogen-activated protein kinases (MAPKs), ERK1 and ERK2, was assessed by Western blotting. Results. GLP-2-treated cells demonstrated a greater than 10-fold increase in proliferation. This response was inhibited by genistein, LY294002, and PD 098059 in a dose-dependent fashion. A significantly greater abundance of the phosphorylated forms of both ERK-1 and ERK-2 was present in cells within 5 rain of treatment with GLP-2. Conclusions. GLP-2 stimulates the proliferation of Caco-2 cells in vitro. This increase in Caco-2 proliferation in response to GLP-2 may be due, at least in part, to the involvement of both the PI 3- kinase and the MAPK pathways. (C) 2000 Academic Press.",
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AU - Jacobs, Danny

AU - Zinner, Michael J.

AU - Ashley, Stanley W.

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N2 - Background. Glucagon-like peptide 2 (GLP-2) stimulates intestinal epithelial growth with high potency and specificity. However, the intracellular signaling pathways responsible for the growth-stimulatory action of GLP-2 are not clearly understood. Here we report possible signaling pathways mediating GLP-2's proliferative actions in the human intestinal epithelial cell line Caco-2. Materials and methods. Caco-2 cells were subcultured under serum-deprived conditions in the presence or absence of GLP-2 (10 μM) and varying concentrations of inhibitors of three candidate kinases: genistein, a global tyrosine kinase inhibitor; LY294002, a phosphatidylinositide (PI) 3-kinase inhibitor; and PD 098059, a mitogen- activated/extracellular signal-regulated kinase (MEK) inhibitor. Proliferation was assessed using [3H]thymidine incorporation. Relative abundance of the phosphorylated forms of two specific mitogen-activated protein kinases (MAPKs), ERK1 and ERK2, was assessed by Western blotting. Results. GLP-2-treated cells demonstrated a greater than 10-fold increase in proliferation. This response was inhibited by genistein, LY294002, and PD 098059 in a dose-dependent fashion. A significantly greater abundance of the phosphorylated forms of both ERK-1 and ERK-2 was present in cells within 5 rain of treatment with GLP-2. Conclusions. GLP-2 stimulates the proliferation of Caco-2 cells in vitro. This increase in Caco-2 proliferation in response to GLP-2 may be due, at least in part, to the involvement of both the PI 3- kinase and the MAPK pathways. (C) 2000 Academic Press.

AB - Background. Glucagon-like peptide 2 (GLP-2) stimulates intestinal epithelial growth with high potency and specificity. However, the intracellular signaling pathways responsible for the growth-stimulatory action of GLP-2 are not clearly understood. Here we report possible signaling pathways mediating GLP-2's proliferative actions in the human intestinal epithelial cell line Caco-2. Materials and methods. Caco-2 cells were subcultured under serum-deprived conditions in the presence or absence of GLP-2 (10 μM) and varying concentrations of inhibitors of three candidate kinases: genistein, a global tyrosine kinase inhibitor; LY294002, a phosphatidylinositide (PI) 3-kinase inhibitor; and PD 098059, a mitogen- activated/extracellular signal-regulated kinase (MEK) inhibitor. Proliferation was assessed using [3H]thymidine incorporation. Relative abundance of the phosphorylated forms of two specific mitogen-activated protein kinases (MAPKs), ERK1 and ERK2, was assessed by Western blotting. Results. GLP-2-treated cells demonstrated a greater than 10-fold increase in proliferation. This response was inhibited by genistein, LY294002, and PD 098059 in a dose-dependent fashion. A significantly greater abundance of the phosphorylated forms of both ERK-1 and ERK-2 was present in cells within 5 rain of treatment with GLP-2. Conclusions. GLP-2 stimulates the proliferation of Caco-2 cells in vitro. This increase in Caco-2 proliferation in response to GLP-2 may be due, at least in part, to the involvement of both the PI 3- kinase and the MAPK pathways. (C) 2000 Academic Press.

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